PMID- 28849055
OWN - NLM
STAT- MEDLINE
DCOM- 20180521
LR  - 20181113
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 16
IP  - 4
DP  - 2017 Oct
TI  - Microarray data re-annotation reveals specific lncRNAs and their potential 
      functions in non-small cell lung cancer subtypes.
PG  - 5129-5136
LID - 10.3892/mmr.2017.7244 [doi]
AB  - Non‑small‑cell lung cancer (NSCLC) is a leading cause of cancer mortality 
      worldwide. The most common subtypes of NSCLC are adenocarcinoma (AC) and squamous 
      cell carcinoma (SCC). However, the pathophysiological mechanisms contributing to 
      AC and SCC are still largely unknown, especially the roles of long non‑coding 
      RNAs (lncRNAs). The present study identified differentially expressed lncRNAs 
      between lung AC and SCC by re‑annotation of NSCLC microarray data analysis 
      profiling. The potential functions of lncRNAs were predicted by using 
      coding‑non‑coding gene co‑expressing network. Reverse transcription-quantitative 
      polymerase chain reaction (RT‑qPCR) was used to investigate lncRNA expression 
      levels in AC cell lines (A549 and L78), SCC cell lines (H226 and H520) and normal 
      cells (NL‑20). Western blotting analysis was used to investigate the protein 
      expression levels in these cell lines. A total of 65 lncRNAs were differentially 
      expressed between AC and SCC including 28 lncRNAs that were downregulated in SCC 
      subtypes compared with those in AC ones, and 37 upregulated lncRNAs in SCC 
      subtypes compared with AC subtypes. Three lncRNAs, sex determining region Y‑box 
      2 overlapping transcript (SOX2‑OT), NCBP2 antisense RNA 2 (NCBP2‑AS2) and 
      ubiquitin like with PHD and ring finger domains 1 (UHRF1), were predicted to be 
      associated with lung cancer; RT‑qPCR confirmed that SOX2‑OT and NCBP2‑AS2 were 
      associated with lung cancer. Finally, western blot assays demonstrated that there 
      was no difference in beta‑catenin and glycogen synthase kinase 3beta (GSK‑3beta) 
      expression in cancer cells compared with NL‑20, but increased phosphorylated 
      (p‑)beta‑catenin and p‑GSK‑3beta was detected in lung cancer cell lines compared with 
      NL‑20, particularly in A549 cells. Although these results require further 
      experimental verification, the analysis of lncRNA signatures between AC and SCC 
      has provided insights into the regulatory mechanism of NSCLC development.
FAU - Zhou, Dongbo
AU  - Zhou D
AD  - Department of Gerontology, Xiangya Hospital of Central South University, 
      Changsha, Hunan 410078, P.R. China.
FAU - Xie, Mingxuan
AU  - Xie M
AD  - Department of Gerontology, Xiangya Hospital of Central South University, 
      Changsha, Hunan 410078, P.R. China.
FAU - He, Baimei
AU  - He B
AD  - Department of Gerontology, Xiangya Hospital of Central South University, 
      Changsha, Hunan 410078, P.R. China.
FAU - Gao, Ying
AU  - Gao Y
AD  - Department of Gerontology, Xiangya Hospital of Central South University, 
      Changsha, Hunan 410078, P.R. China.
FAU - Yu, Qiao
AU  - Yu Q
AD  - Department of Gerontology, Xiangya Hospital of Central South University, 
      Changsha, Hunan 410078, P.R. China.
FAU - He, Bixiu
AU  - He B
AD  - Department of Gerontology, Xiangya Hospital of Central South University, 
      Changsha, Hunan 410078, P.R. China.
FAU - Chen, Qiong
AU  - Chen Q
AD  - Department of Gerontology, Xiangya Hospital of Central South University, 
      Changsha, Hunan 410078, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170814
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Carcinoma, Non-Small-Cell Lung/*genetics/metabolism/pathology
MH  - Cell Line, Tumor
MH  - Cluster Analysis
MH  - *Computational Biology/methods
MH  - *Gene Expression Profiling/methods
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Regulatory Networks
MH  - Humans
MH  - Lung Neoplasms/*genetics/metabolism/pathology
MH  - Molecular Sequence Annotation
MH  - RNA, Long Noncoding/*genetics
MH  - *Transcriptome
MH  - Wnt Signaling Pathway
PMC - PMC5647101
EDAT- 2017/08/30 06:00
MHDA- 2018/05/22 06:00
PMCR- 2017/08/14
CRDT- 2017/08/30 06:00
PHST- 2016/04/22 00:00 [received]
PHST- 2017/04/24 00:00 [accepted]
PHST- 2017/08/30 06:00 [pubmed]
PHST- 2018/05/22 06:00 [medline]
PHST- 2017/08/30 06:00 [entrez]
PHST- 2017/08/14 00:00 [pmc-release]
AID - mmr-16-04-5129 [pii]
AID - 10.3892/mmr.2017.7244 [doi]
PST - ppublish
SO  - Mol Med Rep. 2017 Oct;16(4):5129-5136. doi: 10.3892/mmr.2017.7244. Epub 2017 Aug 
      14.