PMID- 28849105
OWN - NLM
STAT- MEDLINE
DCOM- 20180723
LR  - 20240321
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 16
IP  - 5
DP  - 2017 Nov
TI  - Analysis of gene expression profiling variations induced by 
      hsa‑miR‑145‑5p‑overexpression in laryngeal squamous cell carcinoma cell line 
      Tu‑177.
PG  - 5863-5870
LID - 10.3892/mmr.2017.7360 [doi]
AB  - The present study aimed to investigate the variations of the gene network and 
      biological functions induced by hsa‑miR‑145‑5p in the laryngeal squamous cell 
      carcinoma (LSCC) cell line Tu‑177. A hsa‑miR‑145‑5p‑overexpressed Tu‑177 cell 
      model was established, and the gene expression microarray data of 
      miR‑145‑5p‑overexpressed cells and negative control (NC) cells were analyzed. The 
      differentially expressed genes (DEGs) between two groups were identified, and 
      their potential functions were predicted by functional enrichment analysis. 
      Furthermore, the targets of miR‑145‑5p were identified from the DEGs, and their 
      potential functions and protein‑protein interactions (PPIs) were analyzed. The 
      mRNA expressions of acetyl‑CoA carboxylase beta (ACACB), fibroblast growth factor 
      receptor 1 (FGFR1), protein phosphatase 3 catalytic subunit a (PPP3CA) and spleen 
      associated tyrosine kinase (SYK), were analyzed via quantitative polymerase chain 
      reaction. A total of 1,501 upregulated and 887 downregulated genes were 
      identified in the hsa‑miR‑145‑5p‑overexpressed Tu‑177 cells, compared with the NC 
      cells. Of these DEGs, 164 upregulated and 221 downregulated genes were predicted 
      to be targeted by hsa‑miR‑145‑5p. The upregulated target genes were primarily 
      associated with functions of immunity, whereas the downregulated target genes 
      were significantly enriched in the p53 signaling pathway. In the PPI network 
      consisting of 267 target genes, the upregulated ACACB had the greatest degree and 
      interacted with downregulated genes including PPP3CA and SYK, in addition to 
      upregulated genes, including FGFR1. The mRNA expressions of ACACB and FGFR1were 
      markedly enhanced in miR‑145‑5p‑overexpressed Tu‑177 cells, whereas 
      overexpressing miR‑145‑5p significantly reduced mRNA expression of PPP3CA and 
      SYK. hsa‑miR‑145‑5p may exhibit an anticancer role in LSCC via regulating 
      multiple cell processes, including cell proliferation and invasion, fatty acid 
      metabolism, immunity and p53 signaling pathway. These findings provide novel 
      information for the future investigation of miR‑145‑5p functions in LSCC.
FAU - Ding, Yongxia
AU  - Ding Y
AD  - Nursing College of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
FAU - Wu, Yongyan
AU  - Wu Y
AD  - Shanxi Key Laboratory of Otolaryngology, Head and Neck Cancer, Taiyuan, Shanxi 
      030001, P.R. China.
FAU - Gao, Wei
AU  - Gao W
AD  - Shanxi Key Laboratory of Otolaryngology, Head and Neck Cancer, Taiyuan, Shanxi 
      030001, P.R. China.
FAU - Zhang, Chunming
AU  - Zhang C
AD  - Shanxi Key Laboratory of Otolaryngology, Head and Neck Cancer, Taiyuan, Shanxi 
      030001, P.R. China.
FAU - Zhao, Qinli
AU  - Zhao Q
AD  - Shanxi Key Laboratory of Otolaryngology, Head and Neck Cancer, Taiyuan, Shanxi 
      030001, P.R. China.
FAU - Guo, Huina
AU  - Guo H
AD  - Shanxi Key Laboratory of Otolaryngology, Head and Neck Cancer, Taiyuan, Shanxi 
      030001, P.R. China.
FAU - Qu, Xukuan
AU  - Qu X
AD  - Shanxi Key Laboratory of Otolaryngology, Head and Neck Cancer, Taiyuan, Shanxi 
      030001, P.R. China.
FAU - Wen, Shuxin
AU  - Wen S
AD  - Shanxi Key Laboratory of Otolaryngology, Head and Neck Cancer, Taiyuan, Shanxi 
      030001, P.R. China.
FAU - Wang, Binquan
AU  - Wang B
AD  - Nursing College of Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170824
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (MIRN145 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Computational Biology
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - MicroRNAs/*genetics
MH  - Microarray Analysis
MH  - Protein Interaction Maps/*genetics
MH  - Signal Transduction/genetics
PMC - PMC5865762
EDAT- 2017/08/30 06:00
MHDA- 2018/07/24 06:00
PMCR- 2017/08/24
CRDT- 2017/08/30 06:00
PHST- 2017/04/26 00:00 [received]
PHST- 2017/07/12 00:00 [accepted]
PHST- 2017/08/30 06:00 [pubmed]
PHST- 2018/07/24 06:00 [medline]
PHST- 2017/08/30 06:00 [entrez]
PHST- 2017/08/24 00:00 [pmc-release]
AID - mmr-16-05-5863 [pii]
AID - 10.3892/mmr.2017.7360 [doi]
PST - ppublish
SO  - Mol Med Rep. 2017 Nov;16(5):5863-5870. doi: 10.3892/mmr.2017.7360. Epub 2017 Aug 
      24.