PMID- 28849239
OWN - NLM
STAT- MEDLINE
DCOM- 20180705
LR  - 20220330
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 38
IP  - 4
DP  - 2017 Oct
TI  - HSP47 is associated with the prognosis of laryngeal squamous cell carcinoma by 
      inhibiting cell viability and invasion and promoting apoptosis.
PG  - 2444-2452
LID - 10.3892/or.2017.5893 [doi]
AB  - Heat shock protein 47 (HSP47) is a 47 kDa collagen binding protein that has a 
      close relationship with the development and progression of tumours. However, 
      little is known concerning the expression profile of HSP47 in laryngeal squamous 
      cell carcinoma (LSCC) patients and there is still insufficient data concerning 
      the underlying mechanisms. The aim of the present study was to explore the 
      expression of HSP47 in LSCC and provide an overview of its association with 
      tumourigenicity and clinical prognosis. The expression of HSP47 in LSCC and 
      adjacent non-cancerous laryngeal tissues was assessed via western blotting and 
      immunohistochemical studies. The prognostic signi fi cance of HSP47 expression was 
      analysed using a Kaplan-Meier survival curve. To investigate the influence of 
      HSP47 on the viability, invasion and apoptosis of a LSCC cell line, we performed 
      an in vitro analysis with plasmid vectors and small interfering RNA (siRNA). Our 
      results showed that HSP47 protein expression in the LSCC tissues was markedly 
      decreased compared to that noted in the adjacent non-cancerous tissues, and low 
      expression of HSP47 was correlated with poor prognosis in LSCC patients. 
      Upregulation of HSP47 via plasmid vectors inhibited the proliferation, reduced 
      the invasive ability, increased the sensitivity to cisplatin chemotherapy, 
      promoted apoptosis, and induced the G1 phase arrest of LSCC cells in vitro. The 
      expression of apoptosis-regulating proteins was also altered when HSP47 was 
      upregulated, involving increased expression of cleaved caspase-7/-8/-9, PARP, and 
      Bax and decreased expression of Bcl-2. Our present data suggest that HSP47 is an 
      important prognostic factor and an attractive therapeutic target in LSCC due to 
      its influence on the biological behaviour of LSCC cells.
FAU - Song, Xiaoxiao
AU  - Song X
AD  - Department of Otolaryngology, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, P.R. China.
FAU - Liao, Zhisu
AU  - Liao Z
AD  - Department of Otolaryngology, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, P.R. China.
FAU - Zhou, Chunchun
AU  - Zhou C
AD  - Department of Otolaryngology, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, P.R. China.
FAU - Lin, Renyu
AU  - Lin R
AD  - Department of Otolaryngology, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, P.R. China.
FAU - Lu, Jianglong
AU  - Lu J
AD  - Department of Neurosurgery, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, P.R. China.
FAU - Cai, Lin
AU  - Cai L
AD  - Department of Neurosurgery, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, P.R. China.
FAU - Tan, Xiaohua
AU  - Tan X
AD  - School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and 
      Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang 325000, 
      P.R. China.
FAU - Zeng, Wenjian
AU  - Zeng W
AD  - Department of Otolaryngology, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, P.R. China.
FAU - Lu, Xianghe
AU  - Lu X
AD  - Department of Neurosurgery, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, P.R. China.
FAU - Zheng, Weiming
AU  - Zheng W
AD  - Department of Neurosurgery, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, P.R. China.
FAU - Chen, Jianfu
AU  - Chen J
AD  - Department of Otolaryngology, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, P.R. China.
FAU - Su, Zhipeng
AU  - Su Z
AD  - Department of Neurosurgery, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170809
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (HSP47 Heat-Shock Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (SERPINH1 protein, human)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Apoptosis/genetics
MH  - Carcinogenesis/genetics
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Cell Survival/genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - HSP47 Heat-Shock Proteins/*genetics
MH  - Humans
MH  - Kaplan-Meier Estimate
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Invasiveness/genetics/pathology
MH  - Neoplasm Proteins/genetics
MH  - *Prognosis
EDAT- 2017/08/30 06:00
MHDA- 2018/07/06 06:00
CRDT- 2017/08/30 06:00
PHST- 2016/02/24 00:00 [received]
PHST- 2017/07/24 00:00 [accepted]
PHST- 2017/08/30 06:00 [pubmed]
PHST- 2018/07/06 06:00 [medline]
PHST- 2017/08/30 06:00 [entrez]
AID - 10.3892/or.2017.5893 [doi]
PST - ppublish
SO  - Oncol Rep. 2017 Oct;38(4):2444-2452. doi: 10.3892/or.2017.5893. Epub 2017 Aug 9.