PMID- 28851074
OWN - NLM
STAT- MEDLINE
DCOM- 20180810
LR  - 20180810
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 42
IP  - 1
DP  - 2018 Jan
TI  - Macrophage migration inhibitory factor (MIF) promotes rat airway muscle cell 
      proliferation and migration mediated by ERK1/2 and FAK signaling.
PG  - 75-83
LID - 10.1002/cbin.10863 [doi]
AB  - Macrophage migration inhibitory factor (MIF) is an inflammatory mediator that 
      contributes to asthmatic airway remodeling; however, little is known regarding 
      the effects of MIF on airway smooth muscle cells (ASMCs). In the present study, 
      we found that an enhanced expression of MIF promoted ASMC proliferation, 
      increased the population of cells in the S/G2 phase, downregulated P21 
      expression, and upregulated cyclin D1, cyclin D3, and Cdk6 expression. In 
      addition, the apoptosis of ASMCs was significantly decreased in response to MIF 
      overexpression, compared with the negative control. Moreover, MIF facilitated the 
      migration of ASMCs by upregulating the expression of matrix metalloproteinase 
      (MMP)-2. Finally, we showed that MIF increased the phosphorylation of 
      extracellular regulated protein kinases (ERK) 1/2 and focal adhesion kinase 
      (FAK), which are associated with proliferation and migration. In conclusion, this 
      study demonstrated that MIF overexpression promotes the proliferation and 
      migration of ASMCs by upregulating the activity of the ERK1/2 and FAK signaling 
      pathways in these cells, further indicating that inhibition of MIF may prove to 
      be an effective strategy for treating asthma patients with airway remodeling.
CI  - (c) 2017 International Federation for Cell Biology.
FAU - Lan, Haibing
AU  - Lan H
AD  - Department of the Intensive Care Unit, The Second Af fi liated Hospital of Nanchang 
      University, Nanchang, China.
FAU - Wang, Nan
AU  - Wang N
AD  - Research Center of Clinical Medicine, Nanfang Hospital, Southern Medical 
      University, Guangzhou North Avenue, Guangzhou 510515, China.
AD  - Department of Laboratory Medicine, Guangdong Second Traditional Chinese Medicine 
      Hospital, Guangzhou, Guangdong 510095, China.
FAU - Chen, Yu
AU  - Chen Y
AD  - Department of the Intensive Care Unit, The Second Af fi liated Hospital of Nanchang 
      University, Nanchang, China.
FAU - Wang, Xiaojing
AU  - Wang X
AD  - Research Center of Clinical Medicine, Nanfang Hospital, Southern Medical 
      University, Guangzhou North Avenue, Guangzhou 510515, China.
FAU - Gong, Yuanqi
AU  - Gong Y
AD  - Department of the Intensive Care Unit, The Second Af fi liated Hospital of Nanchang 
      University, Nanchang, China.
FAU - Qi, Xiefei
AU  - Qi X
AD  - Department of the Intensive Care Unit, The Second Af fi liated Hospital of Nanchang 
      University, Nanchang, China.
FAU - Luo, Yaling
AU  - Luo Y
AD  - Department of Respiratory Diseases, Nanfang Hospital, Southern Medical 
      University, Guangzhou, China.
FAU - Yao, Fang
AU  - Yao F
AUID- ORCID: 0000-0002-3640-6139
AD  - Research Center of Clinical Medicine, Nanfang Hospital, Southern Medical 
      University, Guangzhou North Avenue, Guangzhou 510515, China.
LA  - eng
PT  - Journal Article
DEP - 20170920
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Macrophage Migration-Inhibitory Factors)
RN  - EC 2.7.10.2 (Focal Adhesion Kinase 1)
RN  - EC 2.7.10.2 (Ptk2 protein, rat)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 5.3.- (Intramolecular Oxidoreductases)
RN  - EC 5.3.2.1 (Mif protein, rat)
SB  - IM
MH  - Airway Remodeling/*physiology
MH  - Animals
MH  - Apoptosis/physiology
MH  - Asthma/metabolism
MH  - Cell Movement/physiology
MH  - Cell Proliferation/physiology
MH  - Cells, Cultured
MH  - Focal Adhesion Kinase 1/*metabolism
MH  - Intramolecular Oxidoreductases/*metabolism
MH  - *MAP Kinase Signaling System
MH  - Macrophage Migration-Inhibitory Factors/*metabolism
MH  - Mitogen-Activated Protein Kinase 3/metabolism
MH  - Myocytes, Smooth Muscle/cytology/metabolism
MH  - Phosphorylation
MH  - Rats
MH  - Signal Transduction
OTO - NOTNLM
OT  - airway smooth muscle cells
OT  - macrophage migration inhibitory factor
OT  - migration
OT  - proliferation
EDAT- 2017/08/30 06:00
MHDA- 2018/08/11 06:00
CRDT- 2017/08/30 06:00
PHST- 2017/03/29 00:00 [received]
PHST- 2017/08/26 00:00 [accepted]
PHST- 2017/08/30 06:00 [pubmed]
PHST- 2018/08/11 06:00 [medline]
PHST- 2017/08/30 06:00 [entrez]
AID - 10.1002/cbin.10863 [doi]
PST - ppublish
SO  - Cell Biol Int. 2018 Jan;42(1):75-83. doi: 10.1002/cbin.10863. Epub 2017 Sep 20.