PMID- 28851291
OWN - NLM
STAT- MEDLINE
DCOM- 20180511
LR  - 20220408
IS  - 1471-2121 (Electronic)
IS  - 1471-2121 (Linking)
VI  - 18
IP  - 1
DP  - 2017 Aug 29
TI  - Honokiol improved chondrogenesis and suppressed inflammation in human umbilical 
      cord derived mesenchymal stem cells via blocking nuclear factor-kappaB pathway.
PG  - 29
LID - 10.1186/s12860-017-0145-9 [doi]
LID - 29
AB  - BACKGROUND: Cartilage degradation is the significant pathological process in 
      osteoarthritis (OA). Inflammatory cytokines, such as interleukin-1beta (IL-1beta), 
      activate various downstream mediators contributing to OA pathology. Recently, 
      stem cell-based cartilage repair emerges as a potential therapeutic strategy that 
      being widely studied, whereas, the outcome is still far from clinical 
      application. In this study, we focused on an anti-inflammatory agent, honokiol, 
      which is isolated from an herb, investigated the potential effects on human 
      umbilical cord derived mesenchymal stem cells (hUC-MSCs) in IL-1beta stimulation. 
      METHODS: Second passage hUC-MSCs were cultured for multi-differentiation. Flow 
      cytometry, qRT-PCR, von Kossa stain, alcian blue stain and oil red O stain were 
      used for characterization and multi-differentiation determination. Honokiol (5, 
      10, 25, 50 muM) and IL-1beta (10 ng/ml) were applied in hUC-MSCs during 
      chondrogenesis. Analysis was performed by MTT, cell apoptosis evaluation, ELISA 
      assay, qRT-PCR and western blot. RESULTS: hUC-MSC was positive for CD73, CD90 and 
      CD105, but lack of CD34 and CD45. Remarkable osteogenesis, chondrogenesis and 
      adipogenesis were detected in hUC-MSCs. IL-1beta enhanced cell apoptosis and 
      necrosis and activated the expression of caspase-3, cyclooxygenase-2 (COX-2), 
      interleukin-6 (IL-6) and matrix metalloproteinase (MMP)-1, -9, 13 in hUC-MSCs. 
      Moreover, the expression of SRY-related high-mobility group box 9 (SOX-9), 
      aggrecan and col2alpha1 was suppressed. Honokiol relieved these negative impacts 
      induced by IL-1beta and suppressed Nuclear factor-kappaB (NF-kappaB) pathway by 
      downregulating expression of p-IKKalpha/beta, p-IkappaBalpha and p-p65 in dose-dependent and 
      time-dependent manner. CONCLUSIONS: Honokiol improved cell survival and 
      chondrogenesis of hUC-MSCs and inhibited IL-1beta-induced inflammatory response, 
      which suggested that combination of anti-inflammation and stem cell can be a 
      novel strategy for better cartilage repair.
FAU - Wu, Hao
AU  - Wu H
AD  - Department of Orthopaedics, The First Affiliated Hospital, College of Medicine, 
      Xi'an Jiaotong University, Xi'an, 710061, People's Republic of China.
FAU - Yin, Zhanhai
AU  - Yin Z
AD  - Department of Orthopaedics, The First Affiliated Hospital, College of Medicine, 
      Xi'an Jiaotong University, Xi'an, 710061, People's Republic of China.
FAU - Wang, Ling
AU  - Wang L
AD  - Center for Biomedical Engineering and Regenerative Medicine, Frontier Institute 
      of Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, People's 
      Republic of China.
FAU - Li, Feng
AU  - Li F
AD  - Department of Orthopaedics, The First Affiliated Hospital, College of Medicine, 
      Xi'an Jiaotong University, Xi'an, 710061, People's Republic of China.
FAU - Qiu, Yusheng
AU  - Qiu Y
AD  - Department of Orthopaedics, The First Affiliated Hospital, College of Medicine, 
      Xi'an Jiaotong University, Xi'an, 710061, People's Republic of China. 
      yusheng.qiu@mail.xjtu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20170829
PL  - England
TA  - BMC Cell Biol
JT  - BMC cell biology
JID - 100966972
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Biphenyl Compounds)
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (Lignans)
RN  - 0 (NF-kappa B)
RN  - 11513CCO0N (honokiol)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 1.14.99.1 (PTGS2 protein, human)
RN  - EC 3.4.22.- (CASP3 protein, human)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.24.- (Collagenases)
SB  - IM
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Apoptosis/drug effects
MH  - Biphenyl Compounds/*pharmacology
MH  - Caspase 3/genetics/metabolism
MH  - Cell Differentiation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Chondrogenesis/*drug effects
MH  - Collagenases/genetics/metabolism
MH  - Cyclooxygenase 2/genetics/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Inflammation/genetics/*metabolism
MH  - Interleukin-6/metabolism
MH  - Lignans/*pharmacology
MH  - Mesenchymal Stem Cells/cytology
MH  - NF-kappa B/*metabolism
MH  - Signal Transduction/drug effects
PMC - PMC5576244
OTO - NOTNLM
OT  - Cartilage repair
OT  - Honokiol
OT  - Interleukin-1beta
OT  - Mesenchymal stem cell
OT  - Osteoarthritis
COIS- CONSENT FOR PUBLICATION: Not applicable. COMPETING INTERESTS: The authors 
      declared that they have no competing interests. PUBLISHER'S NOTE: Springer Nature 
      remains neutral with regard to jurisdictional claims in published maps and 
      institutional affiliations.
EDAT- 2017/08/31 06:00
MHDA- 2018/05/12 06:00
PMCR- 2017/08/29
CRDT- 2017/08/31 06:00
PHST- 2017/02/19 00:00 [received]
PHST- 2017/08/20 00:00 [accepted]
PHST- 2017/08/31 06:00 [entrez]
PHST- 2017/08/31 06:00 [pubmed]
PHST- 2018/05/12 06:00 [medline]
PHST- 2017/08/29 00:00 [pmc-release]
AID - 10.1186/s12860-017-0145-9 [pii]
AID - 145 [pii]
AID - 10.1186/s12860-017-0145-9 [doi]
PST - epublish
SO  - BMC Cell Biol. 2017 Aug 29;18(1):29. doi: 10.1186/s12860-017-0145-9.