PMID- 28852033
OWN - NLM
STAT- MEDLINE
DCOM- 20190415
LR  - 20190415
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 Aug 29
TI  - FISH-based mitotic and meiotic diakinesis karyotypes of Morus notabilis reveal a 
      chromosomal fusion-fission cycle between mitotic and meiotic phases.
PG  - 9573
LID - 10.1038/s41598-017-10079-6 [doi]
LID - 9573
AB  - Mulberry (Morus spp.), in family Moraceae, is a plant with important economic 
      value. Many polyploid levels of mulberry have been determined. In the present 
      study, the fluorescence in situ hybridization (FISH) technique was applied in 
      Morus notabilis, using four single-copy sequences, telomere repeats, and 5S and 
      25S rDNAs as probes. All the mitotic chromosomes were clearly identified and 
      grouped into seven pairs of homologous chromosomes. Three dot chromosome pairs 
      were distinguished by the FISH patterns of the 25S rDNA probe and a simple 
      sequence repeat (SSR2524). According to the FISH signals, chromosome length and 
      morphology, detailed meiotic diakinesis karyotype was constructed. Interestingly, 
      only six bivalent chromosomes were observed in diakinesis cells. The 25S rDNA 
      probe was used to illustrate chromosome alterations. The results indicated that 
      mitotic chromosomes 5 and 7 fused into diakinesis chromosome 5 during the meiotic 
      phase. In mitotic cells, the fused chromosome 5 broke into chromosomes 5 and 7. A 
      chromosomal fusion-fission cycle between the meiotic and mitotic phases in the 
      same individual is reported here for the first time. This finding will contribute 
      to the understanding of karyotype evolution in plants.
FAU - Xuan, Yahui
AU  - Xuan Y
FAU - Li, Chaoshuo
AU  - Li C
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 
      400715, China.
FAU - Wu, Yue
AU  - Wu Y
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 
      400715, China.
FAU - Ma, Bi
AU  - Ma B
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 
      400715, China.
FAU - Liu, Ruiling
AU  - Liu R
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 
      400715, China.
FAU - Xiang, Zhonghuai
AU  - Xiang Z
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 
      400715, China.
FAU - He, Ningjia
AU  - He N
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 
      400715, China. hejia@swu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170829
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal)
RN  - 130527-23-0 (RNA, ribosomal, 25S)
SB  - IM
MH  - Cell Cycle
MH  - Chromosome Mapping
MH  - *Chromosomes, Plant
MH  - DNA, Ribosomal
MH  - In Situ Hybridization, Fluorescence
MH  - *Karyotype
MH  - Meiosis/*genetics
MH  - Mitosis/*genetics
MH  - Morus/*physiology
MH  - RNA, Ribosomal/*genetics
PMC - PMC5575264
COIS- The authors declare that they have no competing interests.
EDAT- 2017/08/31 06:00
MHDA- 2019/04/16 06:00
PMCR- 2017/08/29
CRDT- 2017/08/31 06:00
PHST- 2016/12/16 00:00 [received]
PHST- 2017/08/04 00:00 [accepted]
PHST- 2017/08/31 06:00 [entrez]
PHST- 2017/08/31 06:00 [pubmed]
PHST- 2019/04/16 06:00 [medline]
PHST- 2017/08/29 00:00 [pmc-release]
AID - 10.1038/s41598-017-10079-6 [pii]
AID - 10079 [pii]
AID - 10.1038/s41598-017-10079-6 [doi]
PST - epublish
SO  - Sci Rep. 2017 Aug 29;7(1):9573. doi: 10.1038/s41598-017-10079-6.