PMID- 28853255
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1000-3061 (Print)
IS  - 1000-3061 (Linking)
VI  - 33
IP  - 8
DP  - 2017 Aug 25
TI  - [Preparation and identification of polyclonal antibodies specific for nsp4 
      protein of porcine reproductive and respiratory syndrome virus].
PG  - 1276-1283
LID - 10.13345/j.cjb.170117 [doi]
AB  - To obtain specific antibodies against nsp4 protein of porcine reproductive and 
      respiratory syndrome virus (PRRSV), nsp4 gene was amplified by RT-PCR and cloned 
      into pET-28a(+) vector, designated pET28a-nsp4. pET28a-nsp4 was transformed into 
      Escherichia coli Trasseta (DE3) cells and expressed after induction of IPTG. 
      SDS-PAGE analysis showed that the recombinant protein was expressed in soluble 
      form with the molecular weight of 26 kDa. The soluble fusion protein in the 
      supernatant was purified using Ni+-NTA affinity chromatography. New Zealand 
      rabbits were immunized by the purified nsp4 and anti-sera against nsp4 were 
      obtained. The titer of polyclonal antibodies was about 106 and showed good 
      specificity and sensitivity in the immunofluorescence assay and Western blotting 
      analysis. The polyclonal antibodies also recognized native nsp4 form PRRSV 
      infected Marc-145 cells, providing a useful tool in PRRSV replication mechanism 
      study.
FAU - Cai, Xinna
AU  - Cai X
AD  - College of Veterinary Medicine and Animal Sciences, Shandong Agricultural 
      University, Tai'an 271000, Shandong, China.
FAU - Tan, Min
AU  - Tan M
AD  - College of Veterinary Medicine and Animal Sciences, Shandong Agricultural 
      University, Tai'an 271000, Shandong, China.
FAU - Cao, Shengliang
AU  - Cao S
AD  - College of Veterinary Medicine and Animal Sciences, Shandong Agricultural 
      University, Tai'an 271000, Shandong, China.
FAU - Huang, Yan
AU  - Huang Y
AD  - College of Veterinary Medicine and Animal Sciences, Shandong Agricultural 
      University, Tai'an 271000, Shandong, China.
FAU - Sun, Fachao
AU  - Sun F
AD  - College of Veterinary Medicine and Animal Sciences, Shandong Agricultural 
      University, Tai'an 271000, Shandong, China.
FAU - Shang, Yingli
AU  - Shang Y
AD  - College of Veterinary Medicine and Animal Sciences, Shandong Agricultural 
      University, Tai'an 271000, Shandong, China.
FAU - Liu, Sidang
AU  - Liu S
AD  - College of Veterinary Medicine and Animal Sciences, Shandong Agricultural 
      University, Tai'an 271000, Shandong, China.
FAU - Xiao, Yihong
AU  - Xiao Y
AD  - College of Veterinary Medicine and Animal Sciences, Shandong Agricultural 
      University, Tai'an 271000, Shandong, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Sheng Wu Gong Cheng Xue Bao
JT  - Sheng wu gong cheng xue bao = Chinese journal of biotechnology
JID - 9426463
RN  - 0 (Antibodies, Viral)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*immunology
MH  - Blotting, Western
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Rabbits
MH  - Recombinant Fusion Proteins/immunology
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Swine
MH  - Viral Nonstructural Proteins/*immunology
OTO - NOTNLM
OT  - identification
OT  - nsp4
OT  - polyclonal antibody
OT  - porcine reproductive and respiratory syndrome virus
EDAT- 2017/08/31 06:00
MHDA- 2018/12/12 06:00
CRDT- 2017/08/31 06:00
PHST- 2017/08/31 06:00 [entrez]
PHST- 2017/08/31 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
AID - 10.13345/j.cjb.170117 [doi]
PST - ppublish
SO  - Sheng Wu Gong Cheng Xue Bao. 2017 Aug 25;33(8):1276-1283. doi: 
      10.13345/j.cjb.170117.