PMID- 28854798 OWN - NLM STAT- MEDLINE DCOM- 20171103 LR - 20171103 IS - 0001-723X (Print) IS - 0001-723X (Linking) VI - 61 IP - 3 DP - 2017 TI - RT-PCR detection of porcine reproductive and respiratory syndrome virus based on the ORF5 gene in mainland China, 2012-2015. PG - 336-340 LID - 10.4149/av_2017_312 [doi] AB - Between January 2012 and December 2015, 13,567 clinical samples were collected from healthy pigs and pigs with a history of respiratory symptoms and/or reproductive disorders in 29 provinces of mainland China to detect porcine reproductive and respiratory syndrome virus (PRRSV). From these samples, 7490 were PRRSV-positive (average value, 55.21%). The annual PRRSV detection rate from 2012 to 2015 for each year is 45.67%, 55.99%, 56.91%, and 59.07%, respectively. Phylogenetic analyses revealed that the percentage of highly pathogenic PRRSV strains had a decreasing yearly trend, whereas the vaccine-like strains showed the opposite trend during the years 2012-2015. These data indicate that while the vaccine contributes to PRRSV prevention in China, it might also lead to serious problems. Specifically, we identified 12 NADC30-like PRRSVs during the years 2014-2015, suggesting potential transmission of the newly emerged PRRSV strains in China. Our data contribute to new information about the epidemiology of PRRSV. FAU - Peng, Z AU - Peng Z FAU - Zhao, T AU - Zhao T FAU - Liang, W AU - Liang W FAU - Song, W AU - Song W FAU - Gao, Z AU - Gao Z FAU - Tang, X AU - Tang X FAU - Chen, H AU - Chen H FAU - Wu, B AU - Wu B LA - eng PT - Journal Article PL - Slovakia TA - Acta Virol JT - Acta virologica JID - 0370401 RN - 0 (Viral Envelope Proteins) RN - 0 (Viral Proteins) RN - 0 (glycoprotein 5, PRRSV) SB - IM MH - Animals MH - China MH - Genetic Variation/genetics MH - Phylogeny MH - Porcine Reproductive and Respiratory Syndrome/*virology MH - Porcine respiratory and reproductive syndrome virus/*genetics MH - Reverse Transcriptase Polymerase Chain Reaction/methods MH - Sequence Analysis, DNA MH - Swine MH - Viral Envelope Proteins/*genetics MH - Viral Proteins/*genetics OTO - NOTNLM OT - porcine reproductive and respiratory syndrome virus; recent profile; RT-PCR detection; ORF5 gene; China. EDAT- 2017/09/01 06:00 MHDA- 2017/11/04 06:00 CRDT- 2017/09/01 06:00 PHST- 2017/09/01 06:00 [entrez] PHST- 2017/09/01 06:00 [pubmed] PHST- 2017/11/04 06:00 [medline] AID - 10.4149/av_2017_312 [doi] PST - ppublish SO - Acta Virol. 2017;61(3):336-340. doi: 10.4149/av_2017_312.