PMID- 28855916
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1664-8021 (Print)
IS  - 1664-8021 (Electronic)
IS  - 1664-8021 (Linking)
VI  - 8
DP  - 2017
TI  - A Glimpse into the Satellite DNA Library in Characidae Fish (Teleostei, 
      Characiformes).
PG  - 103
LID - 10.3389/fgene.2017.00103 [doi]
LID - 103
AB  - Satellite DNA (satDNA) is an abundant fraction of repetitive DNA in eukaryotic 
      genomes and plays an important role in genome organization and evolution. In 
      general, satDNA sequences follow a concerted evolutionary pattern through the 
      intragenomic homogenization of different repeat units. In addition, the satDNA 
      library hypothesis predicts that related species share a series of satDNA 
      variants descended from a common ancestor species, with differential 
      amplification of different satDNA variants. The finding of a same satDNA family 
      in species belonging to different genera within Characidae fish provided the 
      opportunity to test both concerted evolution and library hypotheses. For this 
      purpose, we analyzed here sequence variation and abundance of this satDNA family 
      in ten species, by a combination of next generation sequencing (NGS), PCR and 
      Sanger sequencing, and fluorescence in situ hybridization (FISH). We found 
      extensive between-species variation for the number and size of pericentromeric 
      FISH signals. At genomic level, the analysis of 1000s of DNA sequences obtained 
      by Illumina sequencing and PCR amplification allowed defining 150 haplotypes 
      which were linked in a common minimum spanning tree, where different patterns of 
      concerted evolution were apparent. This also provided a glimpse into the satDNA 
      library of this group of species. In consistency with the library hypothesis, 
      different variants for this satDNA showed high differences in abundance between 
      species, from highly abundant to simply relictual variants.
FAU - Utsunomia, Ricardo
AU  - Utsunomia R
AD  - Department of Morphology, Institute of Biosciences, Sao Paulo State 
      UniversityBotucatu, Brazil.
FAU - Ruiz-Ruano, Francisco J
AU  - Ruiz-Ruano FJ
AD  - Departamento de Genetica, Facultad de Ciencias, Universidad de GranadaGranada, 
      Spain.
FAU - Silva, Duilio M Z A
AU  - Silva DMZA
AD  - Department of Morphology, Institute of Biosciences, Sao Paulo State 
      UniversityBotucatu, Brazil.
FAU - Serrano, Erica A
AU  - Serrano EA
AD  - Department of Morphology, Institute of Biosciences, Sao Paulo State 
      UniversityBotucatu, Brazil.
FAU - Rosa, Ivana F
AU  - Rosa IF
AD  - Department of Morphology, Institute of Biosciences, Sao Paulo State 
      UniversityBotucatu, Brazil.
FAU - Scudeler, Patricia E S
AU  - Scudeler PES
AD  - Department of Morphology, Institute of Biosciences, Sao Paulo State 
      UniversityBotucatu, Brazil.
FAU - Hashimoto, Diogo T
AU  - Hashimoto DT
AD  - CAUNESP, Sao Paulo State UniversityJaboticabal, Brazil.
FAU - Oliveira, Claudio
AU  - Oliveira C
AD  - Department of Morphology, Institute of Biosciences, Sao Paulo State 
      UniversityBotucatu, Brazil.
FAU - Camacho, Juan Pedro M
AU  - Camacho JPM
AD  - Departamento de Genetica, Facultad de Ciencias, Universidad de GranadaGranada, 
      Spain.
FAU - Foresti, Fausto
AU  - Foresti F
AD  - Department of Morphology, Institute of Biosciences, Sao Paulo State 
      UniversityBotucatu, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20170814
PL  - Switzerland
TA  - Front Genet
JT  - Frontiers in genetics
JID - 101560621
PMC - PMC5557728
OTO - NOTNLM
OT  - concerted evolution
OT  - genome evolution
OT  - in situ hybridization
OT  - repetitive DNA
OT  - satellite DNA
EDAT- 2017/09/01 06:00
MHDA- 2017/09/01 06:01
PMCR- 2017/08/14
CRDT- 2017/09/01 06:00
PHST- 2017/07/05 00:00 [received]
PHST- 2017/07/26 00:00 [accepted]
PHST- 2017/09/01 06:00 [entrez]
PHST- 2017/09/01 06:00 [pubmed]
PHST- 2017/09/01 06:01 [medline]
PHST- 2017/08/14 00:00 [pmc-release]
AID - 10.3389/fgene.2017.00103 [doi]
PST - epublish
SO  - Front Genet. 2017 Aug 14;8:103. doi: 10.3389/fgene.2017.00103. eCollection 2017.