PMID- 28856226
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231112
IS  - 2352-5517 (Print)
IS  - 2352-5517 (Electronic)
IS  - 2352-5517 (Linking)
VI  - 8
DP  - 2017 Aug
TI  - Limited comparability of creatinine assays in patients with liver cirrhosis and 
      their impact on the MELD score.
PG  - 41-48
LID - 10.1016/j.plabm.2017.04.002 [doi]
AB  - BACKGROUND & AIM: Patients with end-stage liver disease require valid estimations 
      of mortality for organ allocation and risk stratification. The model of end-stage 
      liver disease (MELD) score is used for this purpose in most countries and 
      incorporates bilirubin, International Normalized ratio, and creatinine. The aim 
      of this study was to evaluate the comparability of creatinine results from 
      different routine assays in the serum samples of patients with liver cirrhosis. 
      METHODS: Residual material from 60 serum samples was available from patients in 
      different stages of liver cirrhosis. Four centers participated; each center 
      analyzed the samples with Jaffe-based and enzymatic routine assays in parallel. 
      In addition, an accredited calibration laboratory certified the panel of samples 
      by an internationally accepted reference measurement procedure (RMP) based on 
      isotope dilution mass spectrometry (ID-MS). This method served as the independent 
      reference. RESULTS: All routine methods displayed a high correlation to the RMP 
      (r >/=0.937, p<0.001). Two enzymatic and two Jaffe-based methods provided results 
      that were all within a +/-20% range of the RMP. The other methods showed deviations 
      >20% in up to 27% of the samples. The enzymatic methods were systematically 
      lower, whereas the Jaffe-based methods were systematically higher (p<0.001). The 
      resulting MELD scores differed from 0 to 4 points. CONCLUSIONS: There are 
      systematic deviations from the RMP. Jaffe-based assays gave higher results, 
      whereas the enzymatic-based assays gave lower results compared to the results of 
      the RMP. The comparability of results is limited and could be disadvantageous to 
      patients listed for liver transplantation.
FAU - Kaiser, Thorsten
AU  - Kaiser T
AD  - Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, 
      University Hospital Leipzig, 04103 Leipzig, Germany.
FAU - Kinny-Koster, Benedict
AU  - Kinny-Koster B
AD  - Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, 
      University Hospital Leipzig, 04103 Leipzig, Germany.
FAU - Gnewuch, Carsten
AU  - Gnewuch C
AD  - Institute for Clinical Chemistry and Laboratory Medicine, Regensburg University 
      Medical Center, Regensburg, Germany.
FAU - Karailieva, Diana
AU  - Karailieva D
AD  - Institute for Clinical Chemistry and Laboratory Medicine, Jena University 
      Hospital, Jena, Germany.
FAU - Kiehntopf, Michael
AU  - Kiehntopf M
AD  - Institute for Clinical Chemistry and Laboratory Medicine, Jena University 
      Hospital, Jena, Germany.
FAU - Kessler, Anja
AU  - Kessler A
AD  - Reference Institute for Bioanalytics, Bonn, Germany.
FAU - Ritter-Sket, Christina
AU  - Ritter-Sket C
AD  - Reference Institute for Bioanalytics, Bonn, Germany.
FAU - Schmidt, Michael
AU  - Schmidt M
AD  - Reference Institute for Bioanalytics, Bonn, Germany.
FAU - Brand, Korbinian
AU  - Brand K
AD  - Hannover Medical University, Institute of Clinical Chemistry, Hannover Medical 
      School, Hannover, Germany.
FAU - Thiery, Joachim
AU  - Thiery J
AD  - Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, 
      University Hospital Leipzig, 04103 Leipzig, Germany.
FAU - Lichtinghagen, Ralf
AU  - Lichtinghagen R
AD  - Hannover Medical University, Institute of Clinical Chemistry, Hannover Medical 
      School, Hannover, Germany.
LA  - eng
PT  - Journal Article
DEP - 20170414
PL  - Netherlands
TA  - Pract Lab Med
JT  - Practical laboratory medicine
JID - 101690848
PMC - PMC5575426
EDAT- 2017/09/01 06:00
MHDA- 2017/09/01 06:01
PMCR- 2017/04/14
CRDT- 2017/09/01 06:00
PHST- 2016/12/26 00:00 [received]
PHST- 2017/04/10 00:00 [revised]
PHST- 2017/04/11 00:00 [accepted]
PHST- 2017/09/01 06:00 [entrez]
PHST- 2017/09/01 06:00 [pubmed]
PHST- 2017/09/01 06:01 [medline]
PHST- 2017/04/14 00:00 [pmc-release]
AID - S2352-5517(16)30060-9 [pii]
AID - 10.1016/j.plabm.2017.04.002 [doi]
PST - epublish
SO  - Pract Lab Med. 2017 Apr 14;8:41-48. doi: 10.1016/j.plabm.2017.04.002. eCollection 
      2017 Aug.