PMID- 28857504
OWN - NLM
STAT- MEDLINE
DCOM- 20180830
LR  - 20181113
IS  - 1601-183X (Electronic)
IS  - 1601-1848 (Print)
IS  - 1601-183X (Linking)
VI  - 17
IP  - 2
DP  - 2018 Feb
TI  - Persistent histone modifications at the BDNF and Cdk-5 promoters following 
      extinction of nicotine-seeking in rats.
PG  - 98-106
LID - 10.1111/gbb.12421 [doi]
AB  - Drugs of addiction lead to a wide range of epigenetic changes at the promoter 
      regions of genes directly implicated in learning and memory processes. We have 
      previously shown that the histone deactylase inhibitor, sodium butyrate (NaB), 
      accelerates the extinction of nicotine-seeking and provides resistance to 
      relapse. Here, we explore the potential molecular mechanisms underlying this 
      effect. Rats received intravenous nicotine or saline self-administration, 
      followed by 6 days of extinction training, with each extinction session followed 
      immediately by treatment with NaB or vehicle. On the last day of extinction, rats 
      were killed and the medial ventral prefrontal cortex retained for chromatin 
      immunoprecipitation and quantitative polymerase chain reaction (qPCR). A history 
      of nicotine exposure significantly decreased H3K14 acetylation at the 
      brain-derived neurotrophic factor (BDNF) exon IV promoter, and this effect was 
      abolished with NaB treatment. In contrast, nicotine self-administration alone, 
      resulted in a significant decrease in histone methylation at the H3K27me3 and 
      H3K9me2 marks in the promoter regions of BDNF exon IV and cyclin-dependent kinase 
      5 (Cdk-5). Quantitative PCR-identified changes in several genes associated with 
      NaB treatment that were independent of nicotine exposure; however, an interaction 
      of nicotine history and NaB treatment was detected only in the expression of BDNF 
      IV and BDNF IX. Together these results suggest that nicotine self-administration 
      leads to a number of epigenetic changes at both the BDNF and Cdk-5 promoters, and 
      that these changes may contribute to the enhanced extinction of nicotine-seeking 
      by NaB.
CI  - (c) 2017 John Wiley & Sons Ltd and International Behavioural and Neural Genetics 
      Society.
FAU - Castino, M R
AU  - Castino MR
AD  - School of Psychology, University of New South Wales, Sydney, Australia.
FAU - Baker-Andresen, D
AU  - Baker-Andresen D
AD  - Queensland Brain Institute, The University of Queensland, Brisbane, Australia.
FAU - Ratnu, V S
AU  - Ratnu VS
AD  - Queensland Brain Institute, The University of Queensland, Brisbane, Australia.
FAU - Shevchenko, G
AU  - Shevchenko G
AD  - Center for Gene Therapy, City of Hope, Duarte, CA, USA.
FAU - Morris, K V
AU  - Morris KV
AD  - Center for Gene Therapy, City of Hope, Duarte, CA, USA.
FAU - Bredy, T W
AU  - Bredy TW
AUID- ORCID: 0000-0003-3280-126X
AD  - Queensland Brain Institute, The University of Queensland, Brisbane, Australia.
FAU - Youngson, N A
AU  - Youngson NA
AD  - School of Medical Sciences, University of New South Wales, Sydney, Australia.
FAU - Clemens, K J
AU  - Clemens KJ
AUID- ORCID: 0000-0003-2709-218X
AD  - School of Psychology, University of New South Wales, Sydney, Australia.
LA  - eng
GR  - R01 AI111139/AI/NIAID NIH HHS/United States
GR  - R01 MH113407/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170928
PL  - England
TA  - Genes Brain Behav
JT  - Genes, brain, and behavior
JID - 101129617
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 6M3C89ZY6R (Nicotine)
RN  - EC 2.7.11.1 (Cyclin-Dependent Kinase 5)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*drug effects/genetics
MH  - Conditioning, Operant/drug effects
MH  - Cyclin-Dependent Kinase 5/*drug effects/genetics
MH  - Drug-Seeking Behavior
MH  - Histone Code/drug effects
MH  - Male
MH  - Memory/drug effects
MH  - Nicotine/*pharmacology
MH  - Promoter Regions, Genetic/*drug effects/genetics
MH  - Rats, Sprague-Dawley
MH  - Self Administration
PMC - PMC5820208
MID - NIHMS931284
OTO - NOTNLM
OT  - BDNF
OT  - cdk-5
OT  - epigenetics
OT  - histone acetylation
OT  - histone methylation
OT  - nicotine
EDAT- 2017/09/01 06:00
MHDA- 2018/08/31 06:00
PMCR- 2018/02/21
CRDT- 2017/09/01 06:00
PHST- 2017/07/23 00:00 [received]
PHST- 2017/08/24 00:00 [revised]
PHST- 2017/08/25 00:00 [accepted]
PHST- 2017/09/01 06:00 [pubmed]
PHST- 2018/08/31 06:00 [medline]
PHST- 2017/09/01 06:00 [entrez]
PHST- 2018/02/21 00:00 [pmc-release]
AID - 10.1111/gbb.12421 [doi]
PST - ppublish
SO  - Genes Brain Behav. 2018 Feb;17(2):98-106. doi: 10.1111/gbb.12421. Epub 2017 Sep 
      28.