PMID- 28858752
OWN - NLM
STAT- MEDLINE
DCOM- 20171002
LR  - 20171002
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1063
DP  - 2017 Sep 15
TI  - Determination of chlorpyrifos and its metabolites in cells and culture media by 
      liquid chromatography-electrospray ionization tandem mass spectrometry.
PG  - 112-117
LID - S1570-0232(17)30396-3 [pii]
LID - 10.1016/j.jchromb.2017.08.010 [doi]
AB  - A sensitive method to simultaneously quantitate chlorpyrifos, chlorpyrifos oxon 
      and the detoxified product 3,5,6-trichloro-2-pyridinol (TCP) was developed using 
      either liquid-liquid extraction for culture media samples, or protein 
      precipitation for cell samples. Multiple reaction monitoring in positive ion mode 
      was applied for the detection of chlorpyrifos and chlorpyrifos oxon, and selected 
      ion recording in negative mode was applied to detect TCP. The method provided 
      linear ranges from 5 to 500, 0.2-20 and 20-2000ng/mL for media samples and from 
      0.5-50, 0.02-2 and 2-200ng/million cells for CPF, CPO and TCP, respectively. The 
      method was validated using selectivity, linearity, precision, accuracy, recovery, 
      stability and dilution tests. All relative standard deviations (RSDs) and 
      relative errors (REs) for QC samples were within 15% (except for LLOQ, within 
      20%). This method has been successfully applied to study the neurotoxicity and 
      metabolism of chlorpyrifos in a human neuronal model.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Yang, Xiangkun
AU  - Yang X
AD  - Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, The 
      University of Georgia, Athens, GA 30602-2352, United States.
FAU - Wu, Xian
AU  - Wu X
AD  - Regenerative Bioscience Center, University of Georgia, Athens, GA 30602, United 
      States.
FAU - Brown, Kyle A
AU  - Brown KA
AD  - Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, The 
      University of Georgia, Athens, GA 30602-2352, United States.
FAU - Le, Thao
AU  - Le T
AD  - Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, The 
      University of Georgia, Athens, GA 30602-2352, United States.
FAU - Stice, Steven L
AU  - Stice SL
AD  - Regenerative Bioscience Center, University of Georgia, Athens, GA 30602, United 
      States.
FAU - Bartlett, Michael G
AU  - Bartlett MG
AD  - Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, The 
      University of Georgia, Athens, GA 30602-2352, United States. Electronic address: 
      mgbart@uga.edu.
LA  - eng
PT  - Journal Article
DEP - 20170818
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Culture Media)
RN  - 0 (Pyridones)
RN  - 5598-15-2 (O,O-diethyl O-3,5,6-trichloro-2-pyridyl phosphate)
RN  - 6515-38-4 (3,5,6-trichloro-2-pyridinol)
RN  - JCS58I644W (Chlorpyrifos)
SB  - IM
MH  - Cell Line
MH  - Chlorpyrifos/*analogs & derivatives/*analysis/metabolism
MH  - Chromatography, Liquid/*methods
MH  - Culture Media/chemistry/metabolism
MH  - Humans
MH  - Linear Models
MH  - Liquid-Liquid Extraction
MH  - Pluripotent Stem Cells/chemistry/metabolism
MH  - Pyridones/*analysis/metabolism
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Electrospray Ionization/methods
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Chlorpyrifos
OT  - LC-MS/MS
OT  - Metabolism
OT  - Neuron
OT  - Neurotoxicity
EDAT- 2017/09/01 06:00
MHDA- 2017/10/03 06:00
CRDT- 2017/09/01 06:00
PHST- 2017/03/08 00:00 [received]
PHST- 2017/07/17 00:00 [revised]
PHST- 2017/08/07 00:00 [accepted]
PHST- 2017/09/01 06:00 [pubmed]
PHST- 2017/10/03 06:00 [medline]
PHST- 2017/09/01 06:00 [entrez]
AID - S1570-0232(17)30396-3 [pii]
AID - 10.1016/j.jchromb.2017.08.010 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Sep 15;1063:112-117. doi: 
      10.1016/j.jchromb.2017.08.010. Epub 2017 Aug 18.