PMID- 28859120
OWN - NLM
STAT- MEDLINE
DCOM- 20171016
LR  - 20181202
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 8
DP  - 2017
TI  - Semi-quantitative MALDI-TOF for antimicrobial susceptibility testing in 
      Staphylococcus aureus.
PG  - e0183899
LID - 10.1371/journal.pone.0183899 [doi]
LID - e0183899
AB  - Antibiotic resistant bacterial infections are a significant problem in the 
      healthcare setting, in many cases requiring the rapid administration of 
      appropriate and effective antibiotic therapy. Diagnostic assays capable of 
      quickly and accurately determining the pathogen resistance profile are therefore 
      crucial to initiate or modify care. Matrix-assisted laser desorption/ionization 
      time-of-flight (MALDI-TOF) mass spectrometry (MS) is a standard method for 
      species identification in many clinical microbiology laboratories and is well 
      positioned to be applied towards antimicrobial susceptibility testing. One 
      recently reported approach utilizes semi-quantitative MALDI-TOF MS for growth 
      rate analysis to provide a resistance profile independent of resistance 
      mechanism. This method was previously successfully applied to Gram-negative 
      pathogens and mycobacteria; here, we evaluated this method with the Gram-positive 
      pathogen Staphylococcus aureus. Specifically, we used 35 strains of S. aureus and 
      four antibiotics to optimize and test the assay, resulting in an overall accuracy 
      rate of 95%. Application of the optimized assay also successfully determined 
      susceptibility from mock blood cultures, allowing both species identification and 
      resistance determination for all four antibiotics within 3 hours of blood culture 
      positivity.
FAU - Maxson, Tucker
AU  - Maxson T
AD  - Diagnostic Systems Division, United States Army Medical Research Institute of 
      Infectious Disease, Fort Detrick, Maryland, United States of America.
FAU - Taylor-Howell, Cheryl L
AU  - Taylor-Howell CL
AD  - Diagnostic Systems Division, United States Army Medical Research Institute of 
      Infectious Disease, Fort Detrick, Maryland, United States of America.
FAU - Minogue, Timothy D
AU  - Minogue TD
AUID- ORCID: 0000-0003-4630-5611
AD  - Diagnostic Systems Division, United States Army Medical Research Institute of 
      Infectious Disease, Fort Detrick, Maryland, United States of America.
LA  - eng
PT  - Journal Article
DEP - 20170831
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Cephalosporins)
RN  - 5E8K9I0O4U (Ciprofloxacin)
RN  - 6Q205EH1VU (Vancomycin)
RN  - 807PW4VQE3 (Cefepime)
RN  - UH95VD7V76 (Oxacillin)
SB  - IM
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Cefepime
MH  - Cephalosporins/pharmacology
MH  - Ciprofloxacin/pharmacology
MH  - *Drug Resistance, Multiple, Bacterial
MH  - Humans
MH  - Microbial Sensitivity Tests
MH  - Oxacillin/pharmacology
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
MH  - Staphylococcal Infections/microbiology
MH  - Staphylococcus aureus/*drug effects/growth & development/isolation & purification
MH  - Vancomycin/pharmacology
PMC - PMC5578647
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/09/01 06:00
MHDA- 2017/10/17 06:00
PMCR- 2017/08/31
CRDT- 2017/09/01 06:00
PHST- 2017/05/04 00:00 [received]
PHST- 2017/08/14 00:00 [accepted]
PHST- 2017/09/01 06:00 [entrez]
PHST- 2017/09/01 06:00 [pubmed]
PHST- 2017/10/17 06:00 [medline]
PHST- 2017/08/31 00:00 [pmc-release]
AID - PONE-D-17-17242 [pii]
AID - 10.1371/journal.pone.0183899 [doi]
PST - epublish
SO  - PLoS One. 2017 Aug 31;12(8):e0183899. doi: 10.1371/journal.pone.0183899. 
      eCollection 2017.