PMID- 28859905
OWN - NLM
STAT- MEDLINE
DCOM- 20190212
LR  - 20190215
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Linking)
VI  - 461
DP  - 2018 Feb 5
TI  - Atrazine suppresses FSH-induced steroidogenesis and LH-dependent expression of 
      ovulatory genes through PDE-cAMP signaling pathway in human cumulus granulosa 
      cells.
PG  - 79-88
LID - S0303-7207(17)30451-3 [pii]
LID - 10.1016/j.mce.2017.08.015 [doi]
AB  - Atrazine (ATR) alters female reproductive functions in different animal species. 
      Here, we analyzed whether ATR disturbs steroidogenic and ovulatory processes in 
      hormone-stimulated human cumulus granulosa cells and mechanism of its action. 
      Results showed that treatment of human cumulus granulosa cells with 20 muM ATR for 
      48 h resulted in lower FSH-stimulated estradiol and progesterone production. ATR 
      reduced mRNA levels of aromatase (CYP19A1), steroidogenic acute regulatory 
      protein (STAR) and luteinizing hormone/choriogonadotropin receptor (LHCGR). 
      Addition of hCG 48 h after FSH and ATR treatment did not trigger maximal 
      expression of the ovulatory genes amphiregulin (AREG) and epiregulin (EREG). 
      Mechanistic experiments showed that ATR activated cPDE and decreased cAMP level. 
      Addition of total PDE and specific PDE4 inhibitors, IBMX and rolipram, prevented 
      ATR's action on CYP19A1 and STAR mRNA expression in FSH-stimulated human cumulus 
      granulosa cells. This study suggests that ATR alters steroidogenesis and 
      ovulatory process in human cumulus granulosa cells jeopardizing female 
      reproduction.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Pogrmic-Majkic, Kristina
AU  - Pogrmic-Majkic K
AD  - Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, 
      Serbia. Electronic address: kristina.pogrmic@dbe.uns.ac.rs.
FAU - Samardzija, Dragana
AU  - Samardzija D
AD  - Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, 
      Serbia.
FAU - Stojkov-Mimic, Natasa
AU  - Stojkov-Mimic N
AD  - Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, 
      Serbia.
FAU - Vukosavljevic, Jelena
AU  - Vukosavljevic J
AD  - Clinic for Gynecology and Obstetrics, Clinical Center of Vojvodina, Novi Sad, 
      Serbia.
FAU - Trninic-Pjevic, Aleksandra
AU  - Trninic-Pjevic A
AD  - Clinic for Gynecology and Obstetrics, Clinical Center of Vojvodina, Novi Sad, 
      Serbia.
FAU - Kopitovic, Vesna
AU  - Kopitovic V
AD  - Clinic for Gynecology and Obstetrics, Clinical Center of Vojvodina, Novi Sad, 
      Serbia.
FAU - Andric, Nebojsa
AU  - Andric N
AD  - Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, 
      Serbia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170830
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Phosphodiesterase Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Steroids)
RN  - 1F7A44V6OU (Colforsin)
RN  - 23583-48-4 (8-Bromo Cyclic Adenosine Monophosphate)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 3.1.4.- (Phosphoric Diester Hydrolases)
RN  - K676NL63N7 (Rolipram)
RN  - QJA9M5H4IM (Atrazine)
RN  - TBT296U68M (1-Methyl-3-isobutylxanthine)
SB  - IM
MH  - 1-Methyl-3-isobutylxanthine/pharmacology
MH  - 8-Bromo Cyclic Adenosine Monophosphate/pharmacology
MH  - Atrazine/*toxicity
MH  - Cell Survival/drug effects
MH  - Colforsin/pharmacology
MH  - Cumulus Cells/drug effects/*metabolism
MH  - Cyclic AMP/*metabolism
MH  - Estradiol/biosynthesis
MH  - Female
MH  - Follicle Stimulating Hormone/*pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Luteinizing Hormone/*metabolism
MH  - Ovulation/drug effects/*genetics
MH  - Phosphodiesterase Inhibitors/pharmacology
MH  - Phosphoric Diester Hydrolases/*metabolism
MH  - Progesterone/biosynthesis
MH  - RNA, Messenger/genetics/metabolism
MH  - Rolipram/pharmacology
MH  - Signal Transduction/drug effects/genetics
MH  - Steroids/*biosynthesis
OTO - NOTNLM
OT  - Atrazine
OT  - Human cumulus granulosa cells
OT  - Ovulatory processes
OT  - Steroidogenesis
OT  - cAMP/PDE
EDAT- 2017/09/02 06:00
MHDA- 2019/02/13 06:00
CRDT- 2017/09/02 06:00
PHST- 2017/05/29 00:00 [received]
PHST- 2017/07/13 00:00 [revised]
PHST- 2017/08/28 00:00 [accepted]
PHST- 2017/09/02 06:00 [pubmed]
PHST- 2019/02/13 06:00 [medline]
PHST- 2017/09/02 06:00 [entrez]
AID - S0303-7207(17)30451-3 [pii]
AID - 10.1016/j.mce.2017.08.015 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2018 Feb 5;461:79-88. doi: 10.1016/j.mce.2017.08.015. Epub 
      2017 Aug 30.