PMID- 28860101
OWN - NLM
STAT- MEDLINE
DCOM- 20180522
LR  - 20201209
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 249
DP  - 2017 Nov
TI  - Development and application of a recombinant M protein-based indirect ELISA for 
      the detection of porcine deltacoronavirus IgG antibodies.
PG  - 76-78
LID - S0166-0934(17)30320-8 [pii]
LID - 10.1016/j.jviromet.2017.08.020 [doi]
AB  - Porcine deltacoronavirus (PDCoV) is a recently identified coronavirus in the 
      genus Deltacoronavirus that can cause enteric disease including diarrhea, 
      vomiting, dehydration and mortality in neonatal piglets. Serological assays to 
      detect anti-PDCoV antibodies are presently limited to certain laboratories and 
      geographic regions. In this study, a recombinant M protein-based indirect 
      enzyme-linked immunosorbent assay (PDCoV-rM ELISA) was developed and utilized to 
      determine the prevalence of anti-PDCoV IgG in Hebei province. The PDCoV-rM ELISA 
      showed no cross-reaction with antisera against transmissible gastroenteritis 
      virus (TGEV), porcine epidemic diarrhea virus (PEDV), porcine rotavirus (PRV), 
      porcine circovirus 2 (PCV2), classical swine fever virus (CSFV) or porcine 
      reproductive and respiratory syndrome virus (PRRSV). The diagnostic sensitivity 
      was 90.6% and the diagnostic specificity was 93.3%. A total of 871 serum samples 
      collected in Hebei from January 2015 to October 2016 were checked for presence of 
      antibodies against PDCoV using the novel PDCoV-rM ELISA. Anti-PDCoV IgG 
      antibodies were detected in 11% (96/871) of the samples and in 25% (10/40) of the 
      investigated farms. The data suggest that PDCoV has a low seroprevalence in pig 
      population in Hebei province, China.
CI  - Copyright (c) 2017. Published by Elsevier B.V.
FAU - Luo, Shang-Xing
AU  - Luo SX
AD  - College of Veterinary Medicine, Agricultural University of Hebei, Baoding, 
      071001, People's Republic of China.
FAU - Fan, Jing-Hui
AU  - Fan JH
AD  - College of Veterinary Medicine, Agricultural University of Hebei, Baoding, 
      071001, People's Republic of China.
FAU - Opriessnig, Tanja
AU  - Opriessnig T
AD  - The Roslin Institute and The Royal (Dick) School of Veterinary Studies, 
      University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, UK.
FAU - Di, Jing-Mei
AU  - Di JM
AD  - College of Veterinary Medicine, Agricultural University of Hebei, Baoding, 
      071001, People's Republic of China.
FAU - Liu, Bao-Jing
AU  - Liu BJ
AD  - College of Veterinary Medicine, Agricultural University of Hebei, Baoding, 
      071001, People's Republic of China.
FAU - Zuo, Yu-Zhu
AU  - Zuo YZ
AD  - College of Veterinary Medicine, Agricultural University of Hebei, Baoding, 
      071001, People's Republic of China; College of Animal Science and Technology, 
      Agricultural University of Hebei, Baoding, 071001, People's Republic of China. 
      Electronic address: zuoyuzhu@163.com.
LA  - eng
PT  - Journal Article
DEP - 20170830
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Antibodies, Viral)
RN  - 0 (Coronavirus M Proteins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (M protein, Porcine epidemic diarrhea virus)
RN  - 0 (M protein, Transmissible gastroenteritis virus)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Matrix Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*blood
MH  - China/epidemiology
MH  - Coronavirus/genetics/*immunology/isolation & purification
MH  - Coronavirus Infections/diagnosis/immunology/*veterinary
MH  - Coronavirus M Proteins
MH  - Cross Reactions
MH  - Enzyme-Linked Immunosorbent Assay/methods/*veterinary
MH  - Immunoglobulin G/*blood
MH  - Phylogeny
MH  - Recombinant Proteins/immunology
MH  - Sensitivity and Specificity
MH  - Seroepidemiologic Studies
MH  - Swine
MH  - Swine Diseases/diagnosis/*epidemiology/immunology/virology
MH  - Viral Matrix Proteins/genetics/*immunology
PMC - PMC7113756
OTO - NOTNLM
OT  - ELISA
OT  - M protein
OT  - Porcine deltacoronavirus
OT  - Serum epidemiology
EDAT- 2017/09/02 06:00
MHDA- 2018/05/23 06:00
PMCR- 2017/08/30
CRDT- 2017/09/02 06:00
PHST- 2017/05/19 00:00 [received]
PHST- 2017/08/25 00:00 [revised]
PHST- 2017/08/26 00:00 [accepted]
PHST- 2017/09/02 06:00 [pubmed]
PHST- 2018/05/23 06:00 [medline]
PHST- 2017/09/02 06:00 [entrez]
PHST- 2017/08/30 00:00 [pmc-release]
AID - S0166-0934(17)30320-8 [pii]
AID - 10.1016/j.jviromet.2017.08.020 [doi]
PST - ppublish
SO  - J Virol Methods. 2017 Nov;249:76-78. doi: 10.1016/j.jviromet.2017.08.020. Epub 
      2017 Aug 30.