PMID- 28860955
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210227
IS  - 1598-2629 (Print)
IS  - 2092-6685 (Electronic)
IS  - 1598-2629 (Linking)
VI  - 17
IP  - 4
DP  - 2017 Aug
TI  - Localization of Serum Amyloid A3 in the Mouse Ovary.
PG  - 261-268
LID - 10.4110/in.2017.17.4.261 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) induces serum amyloid A (SAA) 3 among acute-phase 
      proteins in mouse granulosa cells by activating NF-kappaB signaling via p55 TNF-alpha 
      receptor type 1. However, the localization of SAA3 within the ovary is unknown. 
      Here we investigated ovarian localization of SAA3 in a mouse ovulation model and 
      in response to IL-1beta, a proinflammatory mediator. For the ovulation model, equine 
      chorionic gonadotropin (eCG; 2.5 IU) was administered to mice subcutaneously (sc) 
      to stimulate follicular development on day 25 of age and then 50 h after eCG, 
      human chorionic gonadotropin (hCG; 2.5 IU) was administered sc to induce 
      ovulation. The mouse ovulation model was characterized by the localization of 
      CYP19 mRNA expression to granulosa layers of larger follicles. SAA3 mRNA, 
      determined by in situ hybridization, was broadly expressed throughout the whole 
      ovary. Granulosa layers and small follicles expressed higher SAA3 mRNA compared 
      to thecal-interstitial layers and large follicles, respectively. Interestingly, 
      atretic follicles contained cells expressing intense SAA3 mRNA. After ovulation, 
      SAA3 mRNA expression was intensely evident in ruptured follicles and corpora 
      lutea (CL). The intraperitoneal administration of IL-1beta revealed the intense and 
      extensive appearance of specific cells expressing SAA3 mRNA around follicles and 
      in CL. In addition, Gene Expression Omnibus (GEO) database analysis supported 
      expression pattern of SAA3 mRNA observed in mouse ovulation model. Taken 
      together, SAA3 was broadly distributed through the whole ovary, but intensely 
      expressed in atretic follicles and CL. Furthermore, proinflammatory mediators 
      could trigger the intense appearance of SAA3 around follicles and in CL.
FAU - Choi, Hyeongjwa
AU  - Choi H
AD  - Department of Biochemistry and Cancer Biology, Meharry Medical College, 
      Nashville, TN 37208, USA.
FAU - Ignacio, Rosa Mistica C
AU  - Ignacio RMC
AD  - Department of Biochemistry and Cancer Biology, Meharry Medical College, 
      Nashville, TN 37208, USA.
FAU - Lee, Eun-Sook
AU  - Lee ES
AD  - Department of Pharmaceutical Sciences, College of Pharmacy, Florida A&M 
      University, Tallahassee, FL 32301, USA.
FAU - Roby, Katherine F
AU  - Roby KF
AD  - Center for Reproductive Sciences, University of Kansas Medical Center, Kansas 
      City, KS 66160, USA.
AD  - Department of Anatomy and Cell Biology, University of Kansas Medical Center, 
      Kansas City, KS 66160, USA.
FAU - Terranova, Paul F
AU  - Terranova PF
AD  - Center for Reproductive Sciences, University of Kansas Medical Center, Kansas 
      City, KS 66160, USA.
AD  - Department of Molecular and Integrative Physiology, University of Kansas Medical 
      Center, Kansas City, KS 66160, USA.
AD  - Department of Obstetrics and Gynecology, University of Kansas Medical Center, 
      Kansas City, KS 66160, USA.
FAU - Son, Deok-Soo
AU  - Son DS
AD  - Department of Biochemistry and Cancer Biology, Meharry Medical College, 
      Nashville, TN 37208, USA.
LA  - eng
GR  - U54 CA163069/CA/NCI NIH HHS/United States
GR  - G12 MD007586/MD/NIMHD NIH HHS/United States
GR  - SC1 GM089630/GM/NIGMS NIH HHS/United States
GR  - SC1 AI089073/AI/NIAID NIH HHS/United States
GR  - SC1 CA200519/CA/NCI NIH HHS/United States
GR  - R01 ES024756/ES/NIEHS NIH HHS/United States
PT  - Journal Article
DEP - 20170811
PL  - Korea (South)
TA  - Immune Netw
JT  - Immune network
JID - 101137270
PMC - PMC5577303
OTO - NOTNLM
OT  - Ovary
OT  - Ovulation
OT  - Serum amyloid A
COIS- Conflict of Interest: The authors declare no potential conflicts of interest.
EDAT- 2017/09/02 06:00
MHDA- 2017/09/02 06:01
PMCR- 2017/08/01
CRDT- 2017/09/02 06:00
PHST- 2017/05/04 00:00 [received]
PHST- 2017/07/30 00:00 [revised]
PHST- 2017/08/01 00:00 [accepted]
PHST- 2017/09/02 06:00 [entrez]
PHST- 2017/09/02 06:00 [pubmed]
PHST- 2017/09/02 06:01 [medline]
PHST- 2017/08/01 00:00 [pmc-release]
AID - 10.4110/in.2017.17.4.261 [doi]
PST - ppublish
SO  - Immune Netw. 2017 Aug;17(4):261-268. doi: 10.4110/in.2017.17.4.261. Epub 2017 Aug 
      11.