PMID- 28861754
OWN - NLM
STAT- MEDLINE
DCOM- 20180802
LR  - 20181113
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Linking)
VI  - 42
IP  - 12
DP  - 2017 Dec
TI  - Estrogen Receptor beta Mediated Neuroprotective Efficacy of Cicer microphyllum Seed 
      Extract in Global Hypoxia.
PG  - 3474-3489
LID - 10.1007/s11064-017-2395-5 [doi]
AB  - Hypoxia induced oxidative stress and neurodegeneration in the hippocampus has 
      been implicated for memory impairment in conditions like stroke, ischemia and 
      hypobaric hypoxia. The present study, aimed at investigating the potential of 
      ethanolic extract of Cicer microphyllum seeds (CSE) for amelioration of global 
      hypoxia induced neurodegeneration in CA1 region of hippocampus. CSE 
      supplementation considerably reduced neurodegeneration and dendritic atrophy in 
      CA1 neurons along with improvement of memory in hypoxic rats. This effect of CSE 
      was partly attributed to its antioxidant activity resulting in reduction of lipid 
      peroxidation, protein oxidation and DNA damage during exposure to chronic 
      hypoxia. CSE also promoted dendritic arborization through activation of estrogen 
      receptor beta (ERbeta) and phosphorylation of extracellular signal regulated kinase 
      (ERK1/2) which was independent of brain derived neurotrophic factor (BDNF) 
      mediated signalling mechanisms. Extra nuclear activation of ERK1/2 by ERbeta 
      resulted in phosphorylation of cyclic AMP response element binding protein (CREB) 
      leading to increased expression of PSD-95.These molecular alterations translated 
      to behavioural changes in CSE administered hypoxic animals that performed better 
      in Morris Water Maze Task as compared to vehicle treated hypoxic animals. 
      Toxicological studies show NOEAL > 2000 mg/kg b.w. for oral administration of CSE 
      indicating its safety for consumption. Our findings not only suggest the 
      neuroprotective potential of CSE in hypoxia but also provide evidence for 
      involvement of estrogen receptor and pCREB mediated nootropic effect of the 
      extract.
FAU - Sharma, Deepti
AU  - Sharma D
AD  - Defence Institute of High Altitude Research, Defence Research and Development 
      Organisation, C/o 56 APO, Ladakh, Leh, Jammu And Kashmir, 901205, India.
FAU - Biswal, Surya Narayan
AU  - Biswal SN
AD  - Defence Institute of High Altitude Research, Defence Research and Development 
      Organisation, C/o 56 APO, Ladakh, Leh, Jammu And Kashmir, 901205, India.
FAU - Kumar, Kushal
AU  - Kumar K
AD  - Defence Institute of High Altitude Research, Defence Research and Development 
      Organisation, C/o 56 APO, Ladakh, Leh, Jammu And Kashmir, 901205, India.
FAU - Bhardwaj, Pushpendar
AU  - Bhardwaj P
AD  - Defence Institute of High Altitude Research, Defence Research and Development 
      Organisation, C/o 56 APO, Ladakh, Leh, Jammu And Kashmir, 901205, India.
FAU - Barhwal, Kalpana Kumari
AU  - Barhwal KK
AD  - All India Institute of Medical Sciences, Sijua, Bhubaneswar, Odisha, 751019, 
      India.
FAU - Kumar, Ashish
AU  - Kumar A
AD  - Defence Institute of High Altitude Research, Defence Research and Development 
      Organisation, C/o 56 APO, Ladakh, Leh, Jammu And Kashmir, 901205, India.
FAU - Hota, Sunil Kumar
AU  - Hota SK
AD  - Defence Institute of High Altitude Research, Defence Research and Development 
      Organisation, C/o 56 APO, Ladakh, Leh, Jammu And Kashmir, 901205, India. 
      drsunilhota@hotmail.com.
FAU - Chaurasia, Om Prakash
AU  - Chaurasia OP
AD  - Defence Institute of High Altitude Research, Defence Research and Development 
      Organisation, C/o 56 APO, Ladakh, Leh, Jammu And Kashmir, 901205, India.
LA  - eng
PT  - Journal Article
DEP - 20170831
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Nootropic Agents)
RN  - 0 (Reactive Oxygen Species)
SB  - IM
MH  - Animals
MH  - *Cicer
MH  - Estrogen Receptor beta/*metabolism
MH  - Hippocampus/*metabolism
MH  - Hypoxia/*metabolism
MH  - Male
MH  - Maze Learning/drug effects
MH  - Memory/drug effects/physiology
MH  - Memory Disorders/metabolism
MH  - Neurons/drug effects/metabolism
MH  - Neuroprotection/drug effects
MH  - Nootropic Agents/pharmacology
MH  - Oxidative Stress/drug effects
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species/metabolism
MH  - Seeds
OTO - NOTNLM
OT  - Cicer microphyllum seed extract
OT  - Dendritic arborization
OT  - Estrogen receptorbeta
OT  - Hypoxia
OT  - Memory
OT  - Phytoestrogen
EDAT- 2017/09/02 06:00
MHDA- 2018/08/03 06:00
CRDT- 2017/09/02 06:00
PHST- 2017/03/21 00:00 [received]
PHST- 2017/08/23 00:00 [accepted]
PHST- 2017/08/17 00:00 [revised]
PHST- 2017/09/02 06:00 [pubmed]
PHST- 2018/08/03 06:00 [medline]
PHST- 2017/09/02 06:00 [entrez]
AID - 10.1007/s11064-017-2395-5 [pii]
AID - 10.1007/s11064-017-2395-5 [doi]
PST - ppublish
SO  - Neurochem Res. 2017 Dec;42(12):3474-3489. doi: 10.1007/s11064-017-2395-5. Epub 
      2017 Aug 31.