PMID- 28862540
OWN - NLM
STAT- MEDLINE
DCOM- 20171031
LR  - 20181125
IS  - 1528-7394 (Print)
IS  - 0098-4108 (Linking)
VI  - 80
IP  - 16-18
DP  - 2017
TI  - Characterizing cytotoxic and estrogenic activity of Arctic char tissue extracts 
      in primary Arctic char hepatocytes.
PG  - 1017-1030
LID - 10.1080/15287394.2017.1357277 [doi]
AB  - Contaminants from various anthropogenic activities are detected in the Arctic due 
      to long-range atmospheric transport, ocean currents, and living organisms such as 
      migrating fish or seabirds. Although levels of persistent organic pollutants 
      (POPs) in Arctic fish are generally low, local hot spots of contamination were 
      found in freshwater systems such as Lake Ellasjoen at Bjornoya (Bear Island, 
      Norway). Higher concentrations of organic halogenated compounds (OHC), and higher 
      levels of cytochrome P450 and DNA-double strand breaks were reported in Arctic 
      char (Salvelinus alpinus) from this lake compared to fish from other lakes on 
      Bjornoya. Although several of the measured contaminants are potential endocrine 
      disrupters, few studies have investigated potential endocrine disruptive effects 
      of the contaminant cocktail in this fish population. The aim of this study was to 
      compare acutely toxic and estrogenic potency of the cocktail of pollutants as 
      evidenced by cytotoxic and/or estrogenic effects in vitro using extracts of 
      Arctic char livers from contaminated Lake Ellasjoen with those from less 
      contaminated Lake Laksvatn at Bjornoya. This was performed by in situ sampling 
      and contaminant extraction from liver tissue, followed by chemical analysis and 
      in vitro testing of the following contaminated tissue extracts: F1-nonpolar OHC, 
      F2-polar pesticides and metabolites of OHC, and F3-polar OHC. Contaminant levels 
      were highest in extracts from Ellasjoen fish. The F2 and F3 extracts from Lake 
      Laksvatn and Lake Ellasjoen fish reduced in vitro cell viability at a 
      concentration ratio of 0.03-1 relative to tissue concentration in Arctic char. 
      Only the F3 liver extract from Ellasjoen fish increased in vitro vitellogenin 
      protein expression. Although compounds such as estrogenic OH-PCBs were quantified 
      in Ellasjoen F3 extracts, it remains to be determined which compounds were 
      inducing estrogenic effects.
FAU - Petersen, Karina
AU  - Petersen K
AD  - a Section of Ecotoxicology , Norwegian Institute for Water Research (NIVA) , Oslo 
      , Norway.
FAU - Hultman, Maria T
AU  - Hultman MT
AD  - a Section of Ecotoxicology , Norwegian Institute for Water Research (NIVA) , Oslo 
      , Norway.
FAU - Bytingsvik, Jenny
AU  - Bytingsvik J
AD  - c Department of Arctic R&D , Akvaplan-niva, Fram Centre , Tromso , Norway.
FAU - Harju, Mikael
AU  - Harju M
AD  - b Environmental Chemistry Department , Norwegian Institute for Air Research 
      (NILU) , Tromso , Norway.
FAU - Evenset, Anita
AU  - Evenset A
AD  - c Department of Arctic R&D , Akvaplan-niva, Fram Centre , Tromso , Norway.
AD  - d Department of Arctic and Marine Biology , UiT the Arctic University of Norway , 
      Tromso , Norway.
FAU - Tollefsen, Knut Erik
AU  - Tollefsen KE
AD  - a Section of Ecotoxicology , Norwegian Institute for Water Research (NIVA) , Oslo 
      , Norway.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170901
PL  - England
TA  - J Toxicol Environ Health A
JT  - Journal of toxicology and environmental health. Part A
JID - 100960995
RN  - 0 (Endocrine Disruptors)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Organic Chemicals)
RN  - 0 (Tissue Extracts)
RN  - 0 (Vitellogenins)
RN  - 0 (Water Pollutants, Chemical)
SB  - IM
MH  - Animals
MH  - Arctic Regions
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Endocrine Disruptors/toxicity
MH  - Environmental Monitoring
MH  - Environmental Pollutants/toxicity
MH  - Female
MH  - Hepatocytes/*drug effects/metabolism
MH  - Lakes/chemistry
MH  - Limit of Detection
MH  - Male
MH  - Norway
MH  - Organic Chemicals/*toxicity
MH  - Tissue Extracts/*chemistry
MH  - *Trout
MH  - Vitellogenins/genetics/metabolism
MH  - Water Pollutants, Chemical/toxicity
EDAT- 2017/09/02 06:00
MHDA- 2017/11/01 06:00
CRDT- 2017/09/02 06:00
PHST- 2017/09/02 06:00 [pubmed]
PHST- 2017/11/01 06:00 [medline]
PHST- 2017/09/02 06:00 [entrez]
AID - 10.1080/15287394.2017.1357277 [doi]
PST - ppublish
SO  - J Toxicol Environ Health A. 2017;80(16-18):1017-1030. doi: 
      10.1080/15287394.2017.1357277. Epub 2017 Sep 1.