PMID- 28862740
OWN - NLM
STAT- MEDLINE
DCOM- 20171120
LR  - 20181201
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Print)
IS  - 0009-9104 (Linking)
VI  - 190
IP  - 3
DP  - 2017 Dec
TI  - Dynamics of M1 macrophages in oral mucosal lesions during the development of 
      acute graft-versus-host disease in rats.
PG  - 315-327
LID - 10.1111/cei.13043 [doi]
AB  - The role of macrophage infiltrates in oral mucosal acute graft-versus-host 
      disease (AGVHD) remains unclear, although clinical studies suggest that 
      macrophage infiltration correlates directly with the severity of AGVHD. In this 
      study, we investigated the role of M1 macrophage infiltration in the oral mucosa 
      of rats with AGVHD. Lewis rat spleen cells were injected into (Lewis x Brown 
      Norway) F(1) rats to induce systemic GVHD. Tongue samples were evaluated using 
      histology, immunohistochemistry, dual immunofluorescence, real-time reverse 
      transcription-polymerase chain reaction, Transwell migration assays and 
      Stamper-Woodruff binding assays. At the onset of oral mucosal AGVHD, dual 
      immunofluorescence and migration assays revealed that M1 macrophages had 
      accumulated in the basement membrane (BM) region via the laminin/CD29 beta1 integrin 
      pathway. Macrophage-secreted matrix metalloproteinase-2 was related to BM 
      degradation. The adhesion of macrophages to the oral epithelium could be 
      inhibited by pretreating macrophages with a CC chemokine receptor 2 (CCR2) 
      antibody and/or pretreating lesion sections with monocyte chemoattractant 
      protein-1 (MCP-1) antibody. Our data show that the migration and adhesion of M1 
      macrophages are associated with oral mucosal AGVHD, which is mediated in part by 
      both laminin/CD29 beta 1 intern and MCP-1/CCR2 pathways. Therefore, our study 
      provides additional support for the contribution of macrophage infiltrate to the 
      development of oral mucosal AGVHD.
CI  - (c) 2017 British Society for Immunology.
FAU - Seno, K
AU  - Seno K
AD  - Section of General Dentistry, Department of General Dentistry, Fukuoka Dental 
      College, Sawara-ku, Fukuoka, Japan.
FAU - Yasunaga, M
AU  - Yasunaga M
AD  - Section of Orthodontics, Department of Oral Growth and Development, Fukuoka 
      Dental College, Sawara-ku, Fukuoka, Japan.
AD  - Research Center for Regenerative Medicine, Fukuoka Dental College, Sawara-ku, 
      Fukuoka, Japan.
FAU - Kajiya, H
AU  - Kajiya H
AD  - Research Center for Regenerative Medicine, Fukuoka Dental College, Sawara-ku, 
      Fukuoka, Japan.
AD  - Section of Cellular Physiology, Department of Physiological Science and Molecular 
      Biology, Fukuoka Dental College, Sawara-ku, Fukuoka, Japan.
FAU - Izaki-Hagio, K
AU  - Izaki-Hagio K
AD  - Section of General Dentistry, Department of General Dentistry, Fukuoka Dental 
      College, Sawara-ku, Fukuoka, Japan.
AD  - Research Center for Regenerative Medicine, Fukuoka Dental College, Sawara-ku, 
      Fukuoka, Japan.
FAU - Morita, H
AU  - Morita H
AD  - Section of General Dentistry, Department of General Dentistry, Fukuoka Dental 
      College, Sawara-ku, Fukuoka, Japan.
FAU - Yoneda, M
AU  - Yoneda M
AD  - Section of General Dentistry, Department of General Dentistry, Fukuoka Dental 
      College, Sawara-ku, Fukuoka, Japan.
FAU - Hirofuji, T
AU  - Hirofuji T
AD  - Section of General Dentistry, Department of General Dentistry, Fukuoka Dental 
      College, Sawara-ku, Fukuoka, Japan.
FAU - Ohno, J
AU  - Ohno J
AUID- ORCID: 0000-0002-7790-1603
AD  - Research Center for Regenerative Medicine, Fukuoka Dental College, Sawara-ku, 
      Fukuoka, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170928
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Ccr2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Integrin beta1)
RN  - 0 (Laminin)
RN  - 0 (Receptors, CCR2)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.24 (Mmp2 protein, rat)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Cell Movement/*immunology
MH  - Chemokine CCL2/immunology
MH  - Female
MH  - Graft vs Host Disease/*immunology/pathology
MH  - Integrin beta1/immunology
MH  - Laminin/immunology
MH  - Macrophages/*microbiology/pathology
MH  - Male
MH  - Matrix Metalloproteinase 2/immunology
MH  - Mouth Mucosa/*immunology/pathology
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Receptors, CCR2/immunology
PMC - PMC5680061
OTO - NOTNLM
OT  - CC chemokine receptor 2
OT  - CD29 beta 1 integrin
OT  - M1 macrophages
OT  - acute graft-versus-host disease
OT  - monocyte chemoattractant protein-1
EDAT- 2017/09/02 06:00
MHDA- 2017/11/29 06:00
PMCR- 2018/12/01
CRDT- 2017/09/02 06:00
PHST- 2017/08/26 00:00 [accepted]
PHST- 2017/09/02 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2017/09/02 06:00 [entrez]
PHST- 2018/12/01 00:00 [pmc-release]
AID - CEI13043 [pii]
AID - 10.1111/cei.13043 [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2017 Dec;190(3):315-327. doi: 10.1111/cei.13043. Epub 2017 Sep 
      28.