PMID- 28863735
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Linking)
VI  - 14
IP  - 1
DP  - 2005 Jan
TI  - Regulation of Trophic Factor Expression by Innervating Target Regions in 
      Intraocular Double Transplants.
PG  - 21-29
LID - 10.3727/000000005783983313 [doi]
AB  - Trophic factors have been found to play a significant role both in long-term 
      survival processes and in more rapid and dynamic processes in the brain and 
      spinal cord. However, little is known regarding the regulation of expression of 
      growth factors, and how these proteins interact on a cell-to-cell basis. We have 
      studied protein levels of one growth factor known to affect the noradrenergic 
      innervation of the hippocampal formation, namely brain-derived neurotrophic 
      factor (BDNF). The purpose of the present study was to determine if appropriate 
      innervation or contact between the LC noradrenergic neurons and their target, the 
      hippocampus, affects expression of this growth factor in either brain region. 
      Fetal brain stem tissue, containing the LC, and hippocampal formation were 
      dissected from embryonic day 17 rat fetuses and transplanted together or alone 
      into the anterior chamber of the eye of adult Fisher 344 rats. The tissue was 
      grown together for 6 weeks, after which the animals were sacrificed and ELISAs 
      for BDNF were undertaken. Transplantation to the anterior chamber of the eye 
      increased the expression of BDNF in the hippocampal but not the brain stem 
      tissue, compared with levels observed in fetal and adult rats in vivo. In 
      addition, double grafting with hippocampal tissue more than tripled BDNF levels 
      in brain stem grafts and doubled BDNF levels in the hippocampal portion of double 
      grafts compared with hippocampal single grafts. Triple grafts containing basal 
      forebrain, hippocampus, and brain stem LC tissue increased brain stem and 
      hippocampal BDNF levels even further. Colchicine treatment of LC-hippocampal 
      double grafts gave rise to a significant decrease in hippocampal BDNF levels to 
      levels seen in single hippocampal grafts, while only a partial reduction of BDNF 
      levels was seen in the brain stem portion of the same double grafts treated with 
      colchicine. The findings suggest that an appropriate hippocampal innervation or 
      contact with its target tissues is essential for regulation of BDNF expression in 
      the brain stem, and that retrograde transport of BDNF can occur between double 
      grafted fetal tissues in oculo.
FAU - Willis, L
AU  - Willis L
AD  - Department of Physiology and Neuroscience and the Center on Aging, Medical 
      University of South Carolina, Charleston, SC 29425.
FAU - Quintero, E M
AU  - Quintero EM
AD  - Department of Physiology and Neuroscience and the Center on Aging, Medical 
      University of South Carolina, Charleston, SC 29425.
FAU - Nelson, M
AU  - Nelson M
AD  - Department of Physiology and Neuroscience and the Center on Aging, Medical 
      University of South Carolina, Charleston, SC 29425.
FAU - Granholm, A-Ch
AU  - Granholm AC
AD  - Department of Physiology and Neuroscience and the Center on Aging, Medical 
      University of South Carolina, Charleston, SC 29425.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
OTO - NOTNLM
OT  - BDNF
OT  - Hippocampal formation
OT  - Locus coeruleus
OT  - Neurotrophins
OT  - Transplantation
EDAT- 2005/01/01 00:00
MHDA- 2005/01/01 00:01
CRDT- 2017/09/03 06:00
PHST- 2017/09/03 06:00 [entrez]
PHST- 2005/01/01 00:00 [pubmed]
PHST- 2005/01/01 00:01 [medline]
AID - 10.3727/000000005783983313 [doi]
PST - ppublish
SO  - Cell Transplant. 2005 Jan;14(1):21-29. doi: 10.3727/000000005783983313.