PMID- 28864413
OWN - NLM
STAT- MEDLINE
DCOM- 20171024
LR  - 20171130
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 492
IP  - 3
DP  - 2017 Oct 21
TI  - Effect of sodium fluoride on the sperm mitochondrial DNA in mice.
PG  - 295-299
LID - S0006-291X(17)31712-6 [pii]
LID - 10.1016/j.bbrc.2017.08.129 [doi]
AB  - Previous studies demonstrated that fluoride, as a widespread environmental 
      pollutant, induced the reproductive toxicity at high dose. Besides the decrease 
      of sperm characteristics like concentration, survival, and sperm motility, it was 
      found that high fluoride induced the destructive mitochondrial ultrastructure and 
      decreased ATP production from mitochondrial respiration. However, whether 
      fluoride exposure can damage the mitochondrial DNA (mtDNA) of sperm is still 
      unknown. In this study, 100 male mice were randomly divided into four groups 25 
      each, which were administrated with the distilled water containing 0, 25, 50 and 
      100 mg L(-1) NaF, respectively for 60 days. After exposure, sperm mtDNA copy 
      number was measured by real-time PCR, mtDNA integrity by acridine orange (AO) 
      staining, and nuclear DNA (nDNA) integrity through long PCR amplification, as 
      well as MTCYB and MTATP6 mutations by PCR and direct sequencing. The results 
      showed that 100 mg L(-1) NaF significantly increased sperm mtDNA copy number, and 
      reduced nDNA integrity. There were no changes observed in mtDNA integrity, and 
      mutations of MTCYB and MTATP6 between fluoride groups and the control group. 
      These results indicated that along with low sperm quality, sperm mtDNA copy 
      number is also a sensitive biomarker to reflect the sperm toxicity of fluoride.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Sun, Zilong
AU  - Sun Z
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, Shanxi 030801, PR China; Shanxi Key Laboratory of Ecological 
      Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, Shanxi 030801, PR China. Electronic address: 
      sunzilong2000@outlook.com.
FAU - Xue, Xingchen
AU  - Xue X
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, Shanxi 030801, PR China.
FAU - Zhang, Yuliang
AU  - Zhang Y
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, Shanxi 030801, PR China.
FAU - Niu, Ruiyan
AU  - Niu R
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, Shanxi 030801, PR China; Shanxi Key Laboratory of Ecological 
      Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, Shanxi 030801, PR China.
FAU - Wang, Jundong
AU  - Wang J
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, Shanxi 030801, PR China; Shanxi Key Laboratory of Ecological 
      Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, Shanxi 030801, PR China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170831
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA, Mitochondrial)
RN  - 8ZYQ1474W7 (Sodium Fluoride)
SB  - IM
MH  - Animals
MH  - DNA, Mitochondrial/*drug effects/genetics
MH  - Dose-Response Relationship, Drug
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Sodium Fluoride/administration & dosage/*pharmacology
MH  - Sperm Count
MH  - Spermatozoa/*cytology/*drug effects
MH  - Structure-Activity Relationship
OTO - NOTNLM
OT  - Fluoride
OT  - Mice
OT  - Sperm
OT  - mtDNA
EDAT- 2017/09/03 06:00
MHDA- 2017/10/25 06:00
CRDT- 2017/09/03 06:00
PHST- 2017/08/18 00:00 [received]
PHST- 2017/08/28 00:00 [accepted]
PHST- 2017/09/03 06:00 [pubmed]
PHST- 2017/10/25 06:00 [medline]
PHST- 2017/09/03 06:00 [entrez]
AID - S0006-291X(17)31712-6 [pii]
AID - 10.1016/j.bbrc.2017.08.129 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2017 Oct 21;492(3):295-299. doi: 
      10.1016/j.bbrc.2017.08.129. Epub 2017 Aug 31.