PMID- 28865411
OWN - NLM
STAT- MEDLINE
DCOM- 20190709
LR  - 20220330
IS  - 1735-1502 (Print)
IS  - 1735-1502 (Linking)
VI  - 16
IP  - 4
DP  - 2017 Aug
TI  - Frequency of HLA DQbeta1*0201 and DQbeta1*0301 Alleles and Total Serum IgE in Patients 
      with Bronchial Asthma: A Pilot Study from Pakistan.
PG  - 313-320
AB  - In Pakistan about 3.7% of the population is suffering from asthma, a chronic 
      inflammatory disorder of airways. Asthma has wide spectrum of predisposing 
      factors including environment and genetics. Many studies have been performed to 
      determine association of asthma with serum IgE and major histocompatibility 
      complex (MHC) alleles but conflicting results were reported. Therefore, present 
      study was designed to determine frequency of HLA-DQbeta1*0201 and DQbeta1*0301 alleles 
      in patients with bronchial asthma. This case control study included 85 asthmatic 
      patients and 85 healthy controls. HLA-DQbeta1*0201 and DQbeta1*0301 alleles were 
      detected by allele specific PCR and serum IgE was determined by ELISA. Median and 
      inter-quartile range (IQR) of total IgE level were more increased in asthma 
      patients (585.7 IU/mL and 247.2-848.1 IU/mL) compared to healthy controls (65.1 
      IU/mL and 28.1-181.3 IU/mL) (p<0.001). Frequency of HLA-DQbeta1*0201 and -DQbeta1*0301 
      alleles was more in healthy controls (32% and 38%, p=0.616) as compared to 
      bronchial asthma patients (28% and 26%, p= 0.09). There was a significant 
      association of IgE levels and HLA-DQbeta1*0201 allele. Patients positive for 
      HLA-DQbeta1*0201 allele had low level of serum IgE 357.2 IU/mL (153.9-634.3 IU/mL) 
      compared to the patients negative for this HLA allele i.e. 642.9 IU/mL 
      (289.8-1299.5IU/mL) (p=0.005), whereas, HLA-DQbeta1*0301 allele was not associated 
      with total serum IgE level (p=0.865). Our findings show that HLA-DQbeta1*0201 and 
      -DQbeta1*0301 alleles were not associated with asthma; however, HLA-DQbeta1*0201 allele 
      was associated with low levels of total serum IgE in the study population.
FAU - Mubarak, Bushra
AU  - Mubarak B
AD  - Department of Immunology, University of Health Sciences, Lahore, Pakistan.
FAU - Afzal, Nadeem
AU  - Afzal N
AD  - Department of Immunology, University of Health Sciences, Lahore, Pakistan.
FAU - Javaid, Khursheed
AU  - Javaid K
AD  - Department of Immunology, University of Health Sciences, Lahore, Pakistan.
FAU - Talib, Rakhshanda
AU  - Talib R
AD  - Department of Immunology, University of Health Sciences, Lahore, Pakistan.
FAU - Aslam, Rabia
AU  - Aslam R
AD  - Department of Immunology, University of Health Sciences, Lahore, Pakistan.
FAU - Latif, Waqas
AU  - Latif W
AD  - Department of Immunology, University of Health Sciences, Lahore, Pakistan.
FAU - Khaliq, Saba
AU  - Khaliq S
AD  - Department of Immunology, University of Health Sciences, Lahore, Pakistan 
      AND Department of Physiology and Cell Biology, University of Health Sciences, 
      Lahore, Pakistan.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Allergy Asthma Immunol
JT  - Iranian journal of allergy, asthma, and immunology
JID - 101146178
RN  - 0 (Biomarkers)
RN  - 0 (HLA-DQ beta-Chains)
RN  - 0 (HLA-DQB1 antigen)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - *Alleles
MH  - Asthma/blood/*genetics/*immunology
MH  - Biomarkers
MH  - Blood Cell Count
MH  - Case-Control Studies
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - *Gene Frequency
MH  - HLA-DQ beta-Chains/*genetics
MH  - Histocompatibility Testing
MH  - Humans
MH  - Immunoglobulin E/blood/*immunology
MH  - Male
MH  - Odds Ratio
MH  - Pakistan/epidemiology
MH  - Pilot Projects
MH  - Young Adult
OTO - NOTNLM
OT  - Asthma
OT  - HLA-DQbeta1
OT  - Human leukocyte antigen (HLA)
OT  - Immunoglobulin E
EDAT- 2017/09/04 06:00
MHDA- 2019/07/10 06:00
CRDT- 2017/09/04 06:00
PHST- 2017/08/12 00:00 [accepted]
PHST- 2017/09/04 06:00 [entrez]
PHST- 2017/09/04 06:00 [pubmed]
PHST- 2019/07/10 06:00 [medline]
PST - ppublish
SO  - Iran J Allergy Asthma Immunol. 2017 Aug;16(4):313-320.