PMID- 28867649 OWN - NLM STAT- MEDLINE DCOM- 20180911 LR - 20220311 IS - 1878-7568 (Electronic) IS - 1742-7061 (Linking) VI - 65 DP - 2018 Jan TI - Synthesis and characterization of arginine-NIPAAm hybrid hydrogel as wound dressing: In vitro and in vivo study. PG - 305-316 LID - S1742-7061(17)30560-3 [pii] LID - 10.1016/j.actbio.2017.08.048 [doi] AB - A multi-functional hybrid hydrogel P(M-Arg/NIPAAm) with temperature response, anti-protein adsorption and antibacterial properties was prepared and applied as wound dressing. The hydrogel was carried out by free radical copolymerization of methacrylate arginine (M-Arg) and N-isopropyl acrylamide (NIPAAm) monomers using N,N'-methylene bisacrylamide as a crosslinker, and ammonium persulfate/N,N,N', N'-tetramethylethylenediamine as the redox initiator. To endow the antimicrobial property, chlorhexidine diacetate (CHX) was preloaded into the hydrogel and polyhexamethylene guanidine phosphate (PHMG) was grafted on the hydrogel surface, respectively. The antimicrobial property of two series of hydrogels was evaluated and compared. The successful synthesis of M-Arg, PHMG and hydrogels was proved by (13)C NMR, (1)H NMR and FTIR spectroscopy. The hydrogel morphology characterized by scanning electron microscopy confirmed that the homogeneous porous and interconnected structures of the hydrogels. The swelling, protein adsorption property, in vitro release of CHX, antimicrobial assessment, cell viability as well as in vivo wound healing in a mouse model were studied. The results showed the nontoxicity and antimicrobial P(M-Arg/NIPAAm) hydrogel accelerated the full-thickness wound healing process and had the potential application in wound dressing. STATEMENT OF SIGNIFICANCE: Despite the zwitterionic characteristic and biocompatible property of arginine based hydrogels, the brittle behavior and non-transparency still remain as a significant problem for wound dressing. Furthermore promoting the antibacterial property of the zwitterionic hydrogel is also necessary to prevent the bacterial colonization and subsequent wound infection. Therefore, we created a hybrid hydrogel combined methacrylate arginine (M-Arg) and N-isopropyl acrylamide (NIPAAm). NIPAAm improves transparency and mechanical property as well as acts as a temperature-response drug release system. Additionally, chlorhexidine (CHX) was preloaded into the hydrogels and polyhexamethylene guanidine phosphate (PHMG) was grafted on the hydrogel surface, respectively, which make the hydrogel useful as a favorable antibacterial dressing. The hybrid hydrogel has a combination effect of biocompatibility, environmentally responsive transformation behavior, biodegradability, anti-protein adsorption and antimicrobial properties. This report proposes the preparation of P(M-Arg/NIPAAm) hydrogel that has a great potential for wound healing. CI - Copyright (c) 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. FAU - Wu, De-Qun AU - Wu DQ AD - Key Laboratory of Textile Science & Technology, Ministry Education, College of Textiles, Donghua University, No. 2999 North Renmin Road, Songjiang, Shanghai 201620, China. Electronic address: dqwu@dhu.edu.cn. FAU - Zhu, Jie AU - Zhu J AD - Key Laboratory of Textile Science & Technology, Ministry Education, College of Textiles, Donghua University, No. 2999 North Renmin Road, Songjiang, Shanghai 201620, China. FAU - Han, Hua AU - Han H AD - Key Laboratory of Textile Science & Technology, Ministry Education, College of Textiles, Donghua University, No. 2999 North Renmin Road, Songjiang, Shanghai 201620, China. FAU - Zhang, Jun-Zhi AU - Zhang JZ AD - Key Laboratory of Textile Science & Technology, Ministry Education, College of Textiles, Donghua University, No. 2999 North Renmin Road, Songjiang, Shanghai 201620, China. FAU - Wu, Fei-Fei AU - Wu FF AD - Key Laboratory of Textile Science & Technology, Ministry Education, College of Textiles, Donghua University, No. 2999 North Renmin Road, Songjiang, Shanghai 201620, China. FAU - Qin, Xiao-Hong AU - Qin XH AD - Key Laboratory of Textile Science & Technology, Ministry Education, College of Textiles, Donghua University, No. 2999 North Renmin Road, Songjiang, Shanghai 201620, China. Electronic address: xhqin@dhu.edu.cn. FAU - Yu, Jian-Yong AU - Yu JY AD - Modern Textile Institute, Donghua University, No. 1882 West Yanan Road, Changning, Shanghai 200051, China. LA - eng PT - Journal Article DEP - 20170901 PL - England TA - Acta Biomater JT - Acta biomaterialia JID - 101233144 RN - 0 (Acrylamides) RN - 0 (Anti-Bacterial Agents) RN - 0 (Biocompatible Materials) RN - 0 (Hydrogels) RN - 0 (Proteins) RN - 94ZLA3W45F (Arginine) RN - B7GFF17L9U (N-isopropylacrylamide) RN - R4KO0DY52L (Chlorhexidine) SB - IM MH - Acrylamides/*chemistry MH - Adsorption MH - Animals MH - Anti-Bacterial Agents/pharmacology MH - Arginine/*chemistry MH - *Bandages MH - *Biocompatible Materials MH - Calorimetry, Differential Scanning MH - Carbon-13 Magnetic Resonance Spectroscopy MH - Chlorhexidine/administration & dosage MH - Hydrogels/*chemical synthesis/pharmacology/*therapeutic use MH - Male MH - Mice, Inbred C57BL MH - Microscopy, Electron, Scanning MH - Proteins/chemistry MH - Proton Magnetic Resonance Spectroscopy MH - Spectrophotometry, Ultraviolet MH - Spectroscopy, Fourier Transform Infrared MH - Wound Healing MH - Wounds and Injuries/*therapy OTO - NOTNLM OT - Antimicrobial property OT - Hydrogel dressing OT - Protein absorption OT - Temperature response OT - Wound healing EDAT- 2017/09/05 06:00 MHDA- 2018/09/12 06:00 CRDT- 2017/09/05 06:00 PHST- 2017/02/15 00:00 [received] PHST- 2017/08/17 00:00 [revised] PHST- 2017/08/30 00:00 [accepted] PHST- 2017/09/05 06:00 [pubmed] PHST- 2018/09/12 06:00 [medline] PHST- 2017/09/05 06:00 [entrez] AID - S1742-7061(17)30560-3 [pii] AID - 10.1016/j.actbio.2017.08.048 [doi] PST - ppublish SO - Acta Biomater. 2018 Jan;65:305-316. doi: 10.1016/j.actbio.2017.08.048. Epub 2017 Sep 1.