PMID- 28868076
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1671-5411 (Print)
IS  - 1671-5411 (Linking)
VI  - 14
IP  - 7
DP  - 2017 Jul
TI  - Glycation of high-density lipoprotein triggers oxidative stress and promotes the 
      proliferation and migration of vascular smooth muscle cells.
PG  - 473-480
LID - 10.11909/j.issn.1671-5411.2017.07.003 [doi]
AB  - BACKGROUND: In type 2 diabetes mellitus (T2DM), high-density lipoprotein (HDL) 
      impairs its anti-atherogenic properties and even develops to a pro-inflammatory 
      and pro-atherogenic phenotype because of abnormal compositions and modifications. 
      In this study, we examined the effects and the related mechanisms of glycation of 
      HDL on the proliferation and migration of vascular smooth muscle cells (VSMCs). 
      METHODS & RESULTS: Glycated HDL (G-HDL) was modified with D-glucose (25 mmol/L) 
      in vitro. Diabetic HDL (D-HDL) was isolated from T2DM patients. Rat VSMCs were 
      isolated from the thoracic aortas. Human VSMCs were obtained from ScienCell 
      Research Laboratories. Alpha-actin was detected through immunofluorescence. VSMC 
      proliferation was assayed by Cell Count. VSMC migration was determined by 
      transwell chamber and scratch-wound assay. Intracellular reactive oxygen species 
      (ROS) was detected based on ROS-mediated 2',7'-dichlorofluorescein (DCFH-DA) 
      fluorescence. Compared to native HDL (N-HDL), G-HDL remarkably promoted VSMC 
      proliferation and migration in the dose and time-dependent manners. In addition, 
      G-HDL enhanced ROS generation in VSMCs. However, the ROS scavenger, 
      N-acetylcysteine, efficiently decreased ROS production and subsequently inhibited 
      the proliferation of VSMCs induced by G-HDL. Similarly, D-HDL from T2DM patients 
      also promoted ROS release and VSMC proliferation and migration. CONCLUSIONS: HDL 
      either glycated in vitro or isolated from T2DM patients triggered VSMC 
      proliferation, migration, and oxidative stress. These results might partly 
      interpret the higher morbidity of cardiovascular disease in T2DM patients.
FAU - Du, Qian
AU  - Du Q
AD  - Department of Cardiology, the Affiliated Cardiovascular Hospital of Xiamen 
      University, Medical College of Xiamen University, Xiamen, China.
FAU - Qian, Ming-Ming
AU  - Qian MM
AD  - Department of Cardiology, the Affiliated Cardiovascular Hospital of Xiamen 
      University, Medical College of Xiamen University, Xiamen, China.
FAU - Liu, Pin-Li
AU  - Liu PL
AD  - Department of Endocrinology, the Second Hospital of Hebei Medical University, 
      Shijiazhuang, China.
FAU - Zhang, Le
AU  - Zhang L
AD  - Department of Cardiology, the Affiliated Cardiovascular Hospital of Xiamen 
      University, Medical College of Xiamen University, Xiamen, China.
FAU - Wang, Yan
AU  - Wang Y
AD  - Department of Cardiology, the Affiliated Cardiovascular Hospital of Xiamen 
      University, Medical College of Xiamen University, Xiamen, China.
FAU - Liu, Dong-Hui
AU  - Liu DH
AD  - Department of Cardiology, the Affiliated Cardiovascular Hospital of Xiamen 
      University, Medical College of Xiamen University, Xiamen, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - J Geriatr Cardiol
JT  - Journal of geriatric cardiology : JGC
JID - 101237881
PMC - PMC5545190
OTO - NOTNLM
OT  - Glycation
OT  - High-density lipoprotein
OT  - Migration
OT  - Proliferation
OT  - Vascular smooth muscle cells
EDAT- 2017/09/05 06:00
MHDA- 2017/09/05 06:01
PMCR- 2017/07/01
CRDT- 2017/09/05 06:00
PHST- 2017/09/05 06:00 [entrez]
PHST- 2017/09/05 06:00 [pubmed]
PHST- 2017/09/05 06:01 [medline]
PHST- 2017/07/01 00:00 [pmc-release]
AID - jgc-14-07-473 [pii]
AID - 10.11909/j.issn.1671-5411.2017.07.003 [doi]
PST - ppublish
SO  - J Geriatr Cardiol. 2017 Jul;14(7):473-480. doi: 
      10.11909/j.issn.1671-5411.2017.07.003.