PMID- 28868949 OWN - NLM STAT- MEDLINE DCOM- 20180423 LR - 20181202 IS - 1502-3850 (Electronic) IS - 0001-6357 (Linking) VI - 75 IP - 8 DP - 2017 Nov TI - Cytokine profile in gingival crevicular fluid and plasma of patients with aggressive periodontitis. PG - 616-622 LID - 10.1080/00016357.2017.1372623 [doi] AB - OBJECTIVE: This study aimed to determine the content of cytokines in gingival crevicular fluid (GCF) as well as in plasma of Sudanese patients with aggressive periodontitis (AgP) and healthy controls (HC). MATERIALS AND METHODS: Nineteen AgP patients and 19 HC were included. The mean probing pocket depth and clinical attachment level of the GCF sampled sites in patients were both >/=5 mm. The GCF and plasma levels of 27 cytokines were determined using 27-multiplex fluorescent bead-based immunoassays. Ratios were calculated among cytokines of the T-helper cell subsets Th1 and Th2. Descriptive statistics, the Mann-Whitney U-test and Spearman's rho rank correlation coefficient analysis were used. RESULTS: Interferon-gamma was the only cytokine found in significantly lower levels in GCF of patients compared with HC. Levels of interleukin (IL)-10, IL-13, IL-1Ra, monocyte chemoattractant protein-1 (MCP-1), regulated on activation normal T-cell expressed and secreted (RANTES), granulocyte-colony-stimulating factor (G-CSF), and granulocyte-macrophage-CSF (GM-CSF) were significantly lower in plasma of AgP compared with HC. The ratios of Th1:Th2 in GCF and Treg:Th17 in plasma were significantly lower in AgP. CONCLUSIONS: The lower levels of cytokines detected systemically in plasma of AgP patients may have an impact on the immune response. The lower ratio of Th1:Th2 cytokines in GCF samples of AgP patients suggests a role for Th2 at the local site of disease. FAU - Zein Elabdeen, Hager R AU - Zein Elabdeen HR AD - a Department of Clinical Dentistry - Periodontics , University of Bergen , Bergen , Norway. FAU - Mustafa, Manal AU - Mustafa M AD - a Department of Clinical Dentistry - Periodontics , University of Bergen , Bergen , Norway. AD - b Oral Health Centre of Expertise in Western Norway , Bergen , Norway. FAU - Ali, Raouf AU - Ali R AD - c Faculty of Dentistry , University of Science and Technology , Omdurman , Sudan. FAU - Bolstad, Anne Isine AU - Bolstad AI AD - a Department of Clinical Dentistry - Periodontics , University of Bergen , Bergen , Norway. LA - eng PT - Journal Article DEP - 20170903 PL - Sweden TA - Acta Odontol Scand JT - Acta odontologica Scandinavica JID - 0370344 RN - 0 (Cytokines) RN - 0 (IL10 protein, human) RN - 0 (Interleukin 1 Receptor Antagonist Protein) RN - 130068-27-8 (Interleukin-10) SB - IM MH - Adult MH - Aggressive Periodontitis/diagnosis/*immunology MH - Case-Control Studies MH - Cytokines/*analysis MH - Female MH - Gingival Crevicular Fluid/*chemistry MH - Humans MH - Interleukin 1 Receptor Antagonist Protein/analysis MH - Interleukin-10/analysis MH - Male MH - Middle Aged OTO - NOTNLM OT - GCF OT - Sudan OT - inflammation OT - multiplex immunoassays OT - plasma EDAT- 2017/09/05 06:00 MHDA- 2018/04/24 06:00 CRDT- 2017/09/05 06:00 PHST- 2017/09/05 06:00 [pubmed] PHST- 2018/04/24 06:00 [medline] PHST- 2017/09/05 06:00 [entrez] AID - 10.1080/00016357.2017.1372623 [doi] PST - ppublish SO - Acta Odontol Scand. 2017 Nov;75(8):616-622. doi: 10.1080/00016357.2017.1372623. Epub 2017 Sep 3.