PMID- 28870910
OWN - NLM
STAT- MEDLINE
DCOM- 20171025
LR  - 20171025
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 37
IP  - 9
DP  - 2017 Sep
TI  - Induction and Analysis of Cytotoxic T-Lymphocytes that Recognize Autologous Oral 
      Squamous Cell Carcinoma.
PG  - 4889-4897
AB  - BACKGROUND: It is essential that cytotoxic T-lymphocytes (CTLs) recognize 
      tumor-associated antigens (TAAs) and exhibit antitumor effects in immunological 
      responses for tumor rejection. However, only a few cases with oral squamous cell 
      carcinoma (OSCC) have been studied for these antitumor mechanisms. We established 
      a cancer cell line and autologous CTL pair for identifying TAAs in OSCC, despite 
      difficulties in establishing such a pair. MATERIALS AND METHODS: A cell line 
      (OTM) from a primary lesion and a CTL line (TcOTM) were derived from OSCC in a 
      64-year-old female patient. CTL clones were generated by repetitive limiting 
      dilutions. Accurate characterization was performed by in vitro analysis. RESULTS: 
      The TcOTM clone showed specific cytotoxic activity against OTM cells in an human 
      leukocyte antigen (HLA)-A24-restricted manner. Furthermore, it exerted 
      cytotoxicity against the allogenic HLA-A24 cell lines. CONCLUSION: These data 
      indicated that the TcOTM clone recognized a TAA presented by HLA-A24 cells. This 
      unique method will allow for identification of unknown TAAs for OSCC in the 
      future.
CI  - Copyright(c) 2017, International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Okamoto, Jun-Ya
AU  - Okamoto JY
AD  - Department of Oral Surgery, Sapporo Medical University School of Medicine, 
      Sapporo, Japan.
FAU - Miyazaki, Akihiro
AU  - Miyazaki A
AD  - Department of Oral Surgery, Sapporo Medical University School of Medicine, 
      Sapporo, Japan amiyazak@sapmed.ac.jp.
FAU - Kobayashi, Jun-Ichi
AU  - Kobayashi JI
AD  - Department of Oral Surgery, Sapporo Medical University School of Medicine, 
      Sapporo, Japan.
FAU - Miyamoto, Sho
AU  - Miyamoto S
AD  - Department of Oral Surgery, Sapporo Medical University School of Medicine, 
      Sapporo, Japan.
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
FAU - Sasaki, Takanori
AU  - Sasaki T
AD  - Department of Oral Surgery, Sapporo Medical University School of Medicine, 
      Sapporo, Japan.
FAU - Kanaseki, Takayuki
AU  - Kanaseki T
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
FAU - Hirohashi, Yoshihiko
AU  - Hirohashi Y
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
FAU - Torigoe, Toshihiko
AU  - Torigoe T
AD  - Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, 
      Japan.
FAU - Hiratsuka, Hiroyoshi
AU  - Hiratsuka H
AD  - Department of Oral Surgery, Sapporo Medical University School of Medicine, 
      Sapporo, Japan.
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (HLA-A Antigens)
SB  - IM
MH  - Antigens, Neoplasm/*immunology
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Carcinoma, Squamous Cell/*immunology/metabolism/pathology
MH  - Female
MH  - HLA-A Antigens/*immunology
MH  - Humans
MH  - Lymphocyte Activation
MH  - Middle Aged
MH  - Mouth Neoplasms/*immunology/metabolism/pathology
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Tumor Cells, Cultured
OTO - NOTNLM
OT  - CTL
OT  - HLA-A24
OT  - Oral cancer
OT  - autologous tumor-CTL pair
OT  - oral squamous cell carcinoma
OT  - tumor antigen
EDAT- 2017/09/06 06:00
MHDA- 2017/10/27 06:00
CRDT- 2017/09/06 06:00
PHST- 2017/06/05 00:00 [received]
PHST- 2017/07/03 00:00 [revised]
PHST- 2017/07/05 00:00 [accepted]
PHST- 2017/09/06 06:00 [entrez]
PHST- 2017/09/06 06:00 [pubmed]
PHST- 2017/10/27 06:00 [medline]
AID - 37/9/4889 [pii]
AID - 10.21873/anticanres.11898 [doi]
PST - ppublish
SO  - Anticancer Res. 2017 Sep;37(9):4889-4897. doi: 10.21873/anticanres.11898.