PMID- 28873977
OWN - NLM
STAT- MEDLINE
DCOM- 20171220
LR  - 20171220
IS  - 1945-2403 (Electronic)
IS  - 0146-4760 (Linking)
VI  - 41
IP  - 7
DP  - 2017 Sep 1
TI  - One-Step Derivatization-Extraction Method for Rapid Analysis of Eleven 
      Amphetamines and Cathinones in Oral Fluid by GC-MS.
PG  - 639-645
LID - 10.1093/jat/bkx046 [doi]
AB  - A simple analytical method was developed for in-matrix derivatization of 11 
      amphetamine-like molecules. Ethyl chloroformate as the derivatization reagent and 
      ethyl acetate as the extraction solvent were added directly to oral fluid samples 
      at alkaline pH. Samples were analyzed by gas chromatography-mass spectrometry. 
      Ions monitored for quantification were m/z 44, 91 and 116 for amphetamine (AMP); 
      m/z 58, 91 and 130 for methamphetamine (MA); m/z 44, 105 and 116 for 
      4-methylamphetamine; m/z 44, 116 and 251 for 3,4-methylenedioxyamphetamine; m/z 
      58, 130 and 265 for 3,4-methylenedioxymethamphetamine (MDMA); m/z 72, 116 and 144 
      for 3,4-methylenedioxyethylamphetamine; m/z 44, 105 and 116 for cathinone; m/z 
      58, 105 and 130 for methcathinone; m/z 58, 119 and 130 for mephedrone; m/z 58, 
      107 and 130 for ephedrine (EPH); m/z 207, 250 and 322 for fenethylline; m/z 48, 
      92 and 120 for AMP-D5; m/z 62, 92 and 134 for MA-D5; m/z 48, 120 and 256 for 
      MDA-D5; and m/z 62, 134 and 270 for MDMA-D5. The underlined ions were used as 
      quantifier ions. Calibration curves were linear in the concentration range of 
      2.5-1,000 ng/mL for all analytes except for EPH, which was linear within 5-1,000 
      ng/mL. Precision and accuracy were less than 12.9% (relative standard deviation) 
      and +/-12.8% (bias), respectively, for all analytes. The method was tested in the 
      analysis of oral fluid specimens collected from users.
CI  - (c) The Author 2017. Published by Oxford University Press. All rights reserved. For 
      Permissions, please email: journals.permissions@oup.com.
FAU - Mohamed, Khaled
AU  - Mohamed K
AD  - Forensic Chemistry Department, College of Forensic Sciences, Naif Arab University 
      for Security Sciences, Riyadh, Kingdom of Saudi Arabia.
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Alkaloids)
RN  - 0 (Amphetamines)
RN  - 540EI4406J (cathinone)
SB  - IM
MH  - Alkaloids/*metabolism
MH  - Amphetamines/*metabolism
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Saliva/*metabolism
MH  - Substance Abuse Detection/*methods
EDAT- 2017/09/07 06:00
MHDA- 2017/12/21 06:00
CRDT- 2017/09/07 06:00
PHST- 2016/12/26 00:00 [received]
PHST- 2017/06/14 00:00 [accepted]
PHST- 2017/09/07 06:00 [entrez]
PHST- 2017/09/07 06:00 [pubmed]
PHST- 2017/12/21 06:00 [medline]
AID - 3958768 [pii]
AID - 10.1093/jat/bkx046 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2017 Sep 1;41(7):639-645. doi: 10.1093/jat/bkx046.