PMID- 28874373
OWN - NLM
STAT- MEDLINE
DCOM- 20180611
LR  - 20201209
IS  - 1098-6596 (Electronic)
IS  - 0066-4804 (Print)
IS  - 0066-4804 (Linking)
VI  - 61
IP  - 11
DP  - 2017 Nov
TI  - Hyperbaric Oxygen Sensitizes Anoxic Pseudomonas aeruginosa Biofilm to 
      Ciprofloxacin.
LID - 10.1128/AAC.01024-17 [doi]
LID - e01024-17
AB  - Chronic Pseudomonas aeruginosa lung infection is characterized by the presence of 
      endobronchial antibiotic-tolerant biofilm, which is subject to strong oxygen 
      (O(2)) depletion due to the activity of surrounding polymorphonuclear leukocytes. 
      The exact mechanisms affecting the antibiotic susceptibility of biofilms remain 
      unclear, but accumulating evidence suggests that the efficacy of several 
      bactericidal antibiotics is enhanced by stimulation of aerobic respiration of 
      pathogens, while lack of O(2) increases their tolerance. In fact, the 
      bactericidal effect of several antibiotics depends on active aerobic metabolism 
      activity and the endogenous formation of reactive O(2) radicals (ROS). In this 
      study, we aimed to apply hyperbaric oxygen treatment (HBOT) to sensitize anoxic 
      P. aeruginosa agarose biofilms established to mimic situations with intense O(2) 
      consumption by the host response in the cystic fibrosis (CF) lung. Application of 
      HBOT resulted in enhanced bactericidal activity of ciprofloxacin at clinically 
      relevant durations and was accompanied by indications of restored aerobic 
      respiration, involvement of endogenous lethal oxidative stress, and increased 
      bacterial growth. The findings highlight that oxygenation by HBOT improves the 
      bactericidal activity of ciprofloxacin on P. aeruginosa biofilm and suggest that 
      bacterial biofilms are sensitized to antibiotics by supplying hyperbaric O(2).
CI  - Copyright (c) 2017 Kolpen et al.
FAU - Kolpen, Mette
AU  - Kolpen M
AUID- ORCID: 0000-0001-5575-0384
AD  - Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark 
      mette.kolpen@regionh.dk peter.oestrup.jensen@regionh.dk.
AD  - Department of Immunology and Microbiology, Costerton Biofilm Center, Faculty of 
      Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
FAU - Lerche, Christian J
AU  - Lerche CJ
AD  - Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
FAU - Kragh, Kasper N
AU  - Kragh KN
AD  - Department of Immunology and Microbiology, Costerton Biofilm Center, Faculty of 
      Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
FAU - Sams, Thomas
AU  - Sams T
AD  - Biomedical Engineering, Department of Electrical Engineering, Technical 
      University of Denmark, Lyngby, Denmark.
FAU - Koren, Klaus
AU  - Koren K
AD  - Marine Biological Section, Department of Biology, University of Copenhagen, 
      Helsingor, Denmark.
FAU - Jensen, Anna S
AU  - Jensen AS
AD  - Biomedical Engineering, Department of Electrical Engineering, Technical 
      University of Denmark, Lyngby, Denmark.
FAU - Line, Laura
AU  - Line L
AD  - Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
AD  - Department of Immunology and Microbiology, Costerton Biofilm Center, Faculty of 
      Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
FAU - Bjarnsholt, Thomas
AU  - Bjarnsholt T
AD  - Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
AD  - Department of Immunology and Microbiology, Costerton Biofilm Center, Faculty of 
      Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
FAU - Ciofu, Oana
AU  - Ciofu O
AD  - Department of Immunology and Microbiology, Costerton Biofilm Center, Faculty of 
      Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
FAU - Moser, Claus
AU  - Moser C
AD  - Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
FAU - Kuhl, Michael
AU  - Kuhl M
AD  - Marine Biological Section, Department of Biology, University of Copenhagen, 
      Helsingor, Denmark.
AD  - Climate Change Cluster, University of Technology Sydney, Sydney, Australia.
FAU - Hoiby, Niels
AU  - Hoiby N
AD  - Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
AD  - Department of Immunology and Microbiology, Costerton Biofilm Center, Faculty of 
      Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
FAU - Jensen, Peter O
AU  - Jensen PO
AD  - Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark 
      mette.kolpen@regionh.dk peter.oestrup.jensen@regionh.dk.
AD  - Department of Immunology and Microbiology, Costerton Biofilm Center, Faculty of 
      Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
LA  - eng
PT  - Journal Article
DEP - 20171024
PL  - United States
TA  - Antimicrob Agents Chemother
JT  - Antimicrobial agents and chemotherapy
JID - 0315061
RN  - 0 (Anti-Bacterial Agents)
RN  - 5E8K9I0O4U (Ciprofloxacin)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Anti-Bacterial Agents/pharmacology
MH  - Biofilms/*drug effects
MH  - Ciprofloxacin/*pharmacology
MH  - *Hyperbaric Oxygenation
MH  - Oxygen/pharmacology
MH  - Pseudomonas aeruginosa/*drug effects/physiology
PMC - PMC5655102
OTO - NOTNLM
OT  - Pseudomonas aeruginosa
OT  - biofilms
OT  - ciprofloxacin
OT  - hyperbaric oxygen
OT  - oxygen radicals
EDAT- 2017/09/07 06:00
MHDA- 2018/06/12 06:00
PMCR- 2017/10/24
CRDT- 2017/09/07 06:00
PHST- 2017/05/17 00:00 [received]
PHST- 2017/08/22 00:00 [accepted]
PHST- 2017/09/07 06:00 [pubmed]
PHST- 2018/06/12 06:00 [medline]
PHST- 2017/09/07 06:00 [entrez]
PHST- 2017/10/24 00:00 [pmc-release]
AID - AAC.01024-17 [pii]
AID - 01024-17 [pii]
AID - 10.1128/AAC.01024-17 [doi]
PST - epublish
SO  - Antimicrob Agents Chemother. 2017 Oct 24;61(11):e01024-17. doi: 
      10.1128/AAC.01024-17. Print 2017 Nov.