PMID- 2887443
OWN - NLM
STAT- MEDLINE
DCOM- 19871009
LR  - 20190624
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 138
IP  - 3
DP  - 1987 Jun 26
TI  - [3H]3,4-methylenedioxymethamphetamine (MDMA) interactions with brain membranes 
      and glass fiber filter paper.
PG  - 439-43
AB  - The binding of [3H]3,4-methylenedioxymethamphetamine [( 3H]MDMA) to both rat 
      brain membrane preparations and glass fiber filter papers was analyzed in the 
      present study. Saturation studies indicate that [3H]MDMA binding is saturable and 
      monophasic in both the presence and absence of rat brain membranes. This apparent 
      'specific' binding of [3H]MDMA in both the presence and absence of brain 
      homogenates was totally eliminated by pretreating glass fiber filter papers with 
      polyethylenimine. Drug competition studies demonstrated that [3H]MDMA binding 
      displays a distinct 'pharmacological' profile in the absence of brain tissue. 
      These data indicate that apparent [3H]MDMA binding to rat brain homogenates 
      results from artifactual of [3H]MDMA to glass fiber filter paper. In addition, 
      uptake of [3H]MDMA into rat brain synaptosomes could not be detected. We conclude 
      the [3H]MDMA has limited usefulness in radioligand binding studies.
FAU - Wang, S S
AU  - Wang SS
FAU - Ricaurte, G A
AU  - Ricaurte GA
FAU - Peroutka, S J
AU  - Peroutka SJ
LA  - eng
GR  - NS 12151-12/NS/NINDS NIH HHS/United States
GR  - NS 23560-01/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Amphetamines)
RN  - 10028-17-8 (Tritium)
RN  - 333DO1RDJY (Serotonin)
RN  - 4764-17-4 (3,4-Methylenedioxyamphetamine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - 3,4-Methylenedioxyamphetamine/analogs & derivatives/*metabolism
MH  - Amphetamines/*metabolism
MH  - Animals
MH  - Brain/*metabolism
MH  - Cell Membrane/metabolism
MH  - Filtration
MH  - In Vitro Techniques
MH  - Kinetics
MH  - N-Methyl-3,4-methylenedioxyamphetamine
MH  - Radioligand Assay
MH  - Rats
MH  - Serotonin/metabolism
MH  - Tritium
EDAT- 1987/06/26 00:00
MHDA- 1987/06/26 00:01
CRDT- 1987/06/26 00:00
PHST- 1987/06/26 00:00 [pubmed]
PHST- 1987/06/26 00:01 [medline]
PHST- 1987/06/26 00:00 [entrez]
AID - 0014-2999(87)90485-7 [pii]
AID - 10.1016/0014-2999(87)90485-7 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 1987 Jun 26;138(3):439-43. doi: 10.1016/0014-2999(87)90485-7.