PMID- 28874549
OWN - NLM
STAT- MEDLINE
DCOM- 20180618
LR  - 20181113
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 114
IP  - 42
DP  - 2017 Oct 17
TI  - Molecular basis of mammalian transmissibility of avian H1N1 influenza viruses and 
      their pandemic potential.
PG  - 11217-11222
LID - 10.1073/pnas.1713974114 [doi]
AB  - North American wild birds are an important reservoir of influenza A viruses, yet 
      the potential of viruses in this reservoir to transmit and cause disease in 
      mammals is not well understood. Our surveillance of avian influenza viruses 
      (AIVs) at Delaware Bay, USA, revealed a group of similar H1N1 AIVs isolated in 
      2009, some of which were airborne-transmissible in the ferret model without prior 
      adaptation. Comparison of the genomes of these viruses revealed genetic markers 
      of airborne transmissibility in the Polymerase Basic 2 (PB2), PB1, PB1-F2, 
      Polymerase Acidic-X (PA-X), Nonstructural Protein 1 (NS1), and Nuclear Export 
      Protein (NEP) genes. We studied the role of NS1 in airborne transmission and 
      found that NS1 mutants that were not airborne-transmissible caused limited tissue 
      pathology in the upper respiratory tract (URT). Viral maturation was also 
      delayed, evident as strong intranuclear staining and little virus at the mucosa. 
      Our study of this naturally occurring constellation of genetic markers has 
      provided insights into the poorly understood phenomenon of AIV airborne 
      transmissibility by revealing a role for NS1 and characteristics of viral 
      replication in the URT that were associated with airborne transmission. The 
      transmissibility of these viruses further highlights the pandemic potential of 
      AIVs in the wild bird reservoir and the need to maintain surveillance.
FAU - Zanin, Mark
AU  - Zanin M
AD  - Department of Infectious Diseases, St. Jude Children's Research Hospital, 
      Memphis, TN 38105.
FAU - Wong, Sook-San
AU  - Wong SS
AD  - Department of Infectious Diseases, St. Jude Children's Research Hospital, 
      Memphis, TN 38105.
FAU - Barman, Subrata
AU  - Barman S
AD  - Department of Infectious Diseases, St. Jude Children's Research Hospital, 
      Memphis, TN 38105.
FAU - Kaewborisuth, Challika
AU  - Kaewborisuth C
AD  - Department of Infectious Diseases, St. Jude Children's Research Hospital, 
      Memphis, TN 38105.
FAU - Vogel, Peter
AU  - Vogel P
AD  - Veterinary Pathology Core, St. Jude Children's Research Hospital, Memphis, TN 
      38105.
FAU - Rubrum, Adam
AU  - Rubrum A
AD  - Department of Infectious Diseases, St. Jude Children's Research Hospital, 
      Memphis, TN 38105.
FAU - Darnell, Daniel
AU  - Darnell D
AD  - Department of Infectious Diseases, St. Jude Children's Research Hospital, 
      Memphis, TN 38105.
FAU - Marinova-Petkova, Atanaska
AU  - Marinova-Petkova A
AD  - Department of Infectious Diseases, St. Jude Children's Research Hospital, 
      Memphis, TN 38105.
FAU - Krauss, Scott
AU  - Krauss S
AD  - Department of Infectious Diseases, St. Jude Children's Research Hospital, 
      Memphis, TN 38105.
FAU - Webby, Richard J
AU  - Webby RJ
AD  - Department of Infectious Diseases, St. Jude Children's Research Hospital, 
      Memphis, TN 38105.
FAU - Webster, Robert G
AU  - Webster RG
AD  - Department of Infectious Diseases, St. Jude Children's Research Hospital, 
      Memphis, TN 38105; robert.webster@stjude.org.
LA  - eng
GR  - HHSN266200700005C/AI/NIAID NIH HHS/United States
GR  - HHSN272201400006C/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20170905
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
SB  - IM
CIN - Proc Natl Acad Sci U S A. 2017 Oct 17;114(42):11012-11014. PMID: 29073013
MH  - Animals
MH  - Charadriiformes/*virology
MH  - Chick Embryo
MH  - Disease Vectors
MH  - Ferrets
MH  - Influenza A Virus, H1N1 Subtype/genetics/*pathogenicity
MH  - Male
MH  - Orthomyxoviridae Infections/*transmission
MH  - Respiratory System/virology
MH  - Virus Replication
PMC - PMC5651783
OTO - NOTNLM
OT  - H1N1
OT  - airborne transmission
OT  - avian influenza virus
OT  - ferret
OT  - nonstructural protein 1
COIS- The authors declare no conflict of interest.
EDAT- 2017/09/07 06:00
MHDA- 2018/06/19 06:00
PMCR- 2018/04/17
CRDT- 2017/09/07 06:00
PHST- 2017/09/07 06:00 [pubmed]
PHST- 2018/06/19 06:00 [medline]
PHST- 2017/09/07 06:00 [entrez]
PHST- 2018/04/17 00:00 [pmc-release]
AID - 1713974114 [pii]
AID - 201713974 [pii]
AID - 10.1073/pnas.1713974114 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2017 Oct 17;114(42):11217-11222. doi: 
      10.1073/pnas.1713974114. Epub 2017 Sep 5.