PMID- 28880687
OWN - NLM
STAT- MEDLINE
DCOM- 20180904
LR  - 20220408
IS  - 1439-7609 (Electronic)
IS  - 1439-7595 (Linking)
VI  - 28
IP  - 3
DP  - 2018 May
TI  - TNF-alpha potentiates uric acid-induced interleukin-1beta (IL-1beta) secretion in human 
      neutrophils.
PG  - 513-517
LID - 10.1080/14397595.2017.1369924 [doi]
AB  - OBJECTIVE: Monosodium urate (MSU) has been shown to promote interleukin-1beta 
      (IL-1beta) secretion in human monocytes, but the priming signals for NOD-like 
      receptor family pyrin domain containing 3 (NLRP3) inflammasome pathway remains 
      elusive. In this study, we investigated the role of Tumor necrosis factor-alpha 
      (TNF-alpha) on MSU-mediated IL-1beta induction in human neutrophils. METHODS: Human 
      neutrophils were stimulated with MSU, in the presence or absence of TNF-alpha 
      priming. The cellular supernatants were analyzed for IL-1beta, IL-18, and caspase-1 
      by enzyme-linked immunosorbent assay (ELISA) methods. Pro-IL-1beta mRNA expressions 
      in human neutrophils were analyzed by real-time PCR method. RESULTS: TNF-alpha 
      stimulation induced pro-IL-1beta mRNA expression; however, MSU stimulation did not 
      induce pro-IL-1beta mRNA expression in human neutrophils. TNF-alpha alone or MSU 
      stimulation did not result in efficient IL-1beta secretion in human neutrophils, 
      whereas in TNF-alpha-primed neutrophils, MSU stimulation resulted in a marked IL-1beta 
      and IL-18 secretion. TNF-alpha-primed neutrophils secreted cleaved caspase-1 (p20), 
      in response to MSU stimulation. CONCLUSION: Our data demonstrate that priming of 
      human neutrophils with TNF-alpha promotes uric acid-mediated IL-1beta secretion in the 
      absence of microbial stimulation. These findings provide insights into the 
      neutrophils-mediated inflammatory processes in gouty arthritis.
FAU - Yokose, Kohei
AU  - Yokose K
AD  - a Department of Rheumatology , Fukushima Medical University School of Medicine , 
      Fukushima , Japan.
FAU - Sato, Shuzo
AU  - Sato S
AD  - a Department of Rheumatology , Fukushima Medical University School of Medicine , 
      Fukushima , Japan.
FAU - Asano, Tomoyuki
AU  - Asano T
AD  - a Department of Rheumatology , Fukushima Medical University School of Medicine , 
      Fukushima , Japan.
FAU - Yashiro, Makiko
AU  - Yashiro M
AD  - a Department of Rheumatology , Fukushima Medical University School of Medicine , 
      Fukushima , Japan.
FAU - Kobayashi, Hiroko
AU  - Kobayashi H
AD  - a Department of Rheumatology , Fukushima Medical University School of Medicine , 
      Fukushima , Japan.
FAU - Watanabe, Hiroshi
AU  - Watanabe H
AD  - a Department of Rheumatology , Fukushima Medical University School of Medicine , 
      Fukushima , Japan.
FAU - Suzuki, Eiji
AU  - Suzuki E
AD  - a Department of Rheumatology , Fukushima Medical University School of Medicine , 
      Fukushima , Japan.
FAU - Sato, Chikako
AU  - Sato C
AD  - a Department of Rheumatology , Fukushima Medical University School of Medicine , 
      Fukushima , Japan.
FAU - Kozuru, Hideko
AU  - Kozuru H
AD  - b Clinical Research Center , NHO Nagasaki Medical Center , Omura , Nagasaki , 
      Japan.
FAU - Yatsuhashi, Hiroshi
AU  - Yatsuhashi H
AD  - b Clinical Research Center , NHO Nagasaki Medical Center , Omura , Nagasaki , 
      Japan.
FAU - Migita, Kiyoshi
AU  - Migita K
AD  - a Department of Rheumatology , Fukushima Medical University School of Medicine , 
      Fukushima , Japan.
LA  - eng
PT  - Journal Article
DEP - 20170914
PL  - England
TA  - Mod Rheumatol
JT  - Modern rheumatology
JID - 100959226
RN  - 0 (Interleukin-1beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 268B43MJ25 (Uric Acid)
SB  - IM
MH  - Arthritis, Gouty/metabolism
MH  - Cells, Cultured
MH  - Humans
MH  - Interleukin-1beta/*metabolism
MH  - Neutrophils/drug effects/*metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Uric Acid/*pharmacology
OTO - NOTNLM
OT  - Caspase-1
OT  - IL-1beta
OT  - TNF-alpha
OT  - gout
OT  - inflammasome
OT  - monosodium urate
EDAT- 2017/09/08 06:00
MHDA- 2018/09/05 06:00
CRDT- 2017/09/08 06:00
PHST- 2017/09/08 06:00 [pubmed]
PHST- 2018/09/05 06:00 [medline]
PHST- 2017/09/08 06:00 [entrez]
AID - 10.1080/14397595.2017.1369924 [doi]
PST - ppublish
SO  - Mod Rheumatol. 2018 May;28(3):513-517. doi: 10.1080/14397595.2017.1369924. Epub 
      2017 Sep 14.