PMID- 28880937
OWN - NLM
STAT- MEDLINE
DCOM- 20171016
LR  - 20181202
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 9
DP  - 2017
TI  - Differential expression of tetraspanin superfamily members in dendritic cell 
      subsets.
PG  - e0184317
LID - 10.1371/journal.pone.0184317 [doi]
LID - e0184317
AB  - Dendritic cells (DCs), which are essential for initiating immune responses, are 
      comprised of different subsets. Tetraspanins organize dendritic cell membranes by 
      facilitating protein-protein interactions within the so called tetraspanin web. 
      In this study we analyzed expression of the complete tetraspanin superfamily in 
      primary murine (CD4+, CD8+, pDC) and human DC subsets (CD1c+, CD141+, pDC) at the 
      transcriptome and proteome level. Different RNA and protein expression profiles 
      for the tetraspanin genes across human and murine DC subsets were identified. 
      Although RNA expression levels of CD37 and CD82 were not significantly different 
      between human DC subsets, CD9 RNA was highly expressed in pDCs, while CD9 protein 
      expression was lower. This indicates that relative RNA and protein expression 
      levels are not always in agreement. Both murine CD8alpha+ DCs and its regarded human 
      counterpart, CD141+ DCs, displayed relatively high protein levels of CD81. CD53 
      protein was highly expressed on human pDCs in contrast to the relatively low 
      protein expression of most other tetraspanins. This study demonstrates that 
      tetraspanins are differentially expressed by human and murine DC subsets which 
      provides a valuable resource that will aid the understanding of tetraspanin 
      function in DC biology.
FAU - Zuidscherwoude, Malou
AU  - Zuidscherwoude M
AUID- ORCID: 0000-0002-5071-9000
AD  - Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, 
      Radboud University Medical Center, Nijmegen, The Netherlands.
FAU - Worah, Kuntal
AU  - Worah K
AD  - Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, 
      Radboud University Medical Center, Nijmegen, The Netherlands.
AD  - Centre for Molecular and Biomolecular Informatics (CMBI), Radboud University 
      Medical Center, Nijmegen, the Netherlands.
FAU - van der Schaaf, Alie
AU  - van der Schaaf A
AD  - Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, 
      Radboud University Medical Center, Nijmegen, The Netherlands.
FAU - Buschow, Sonja I
AU  - Buschow SI
AD  - Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, 
      Radboud University Medical Center, Nijmegen, The Netherlands.
AD  - Department of Gastroenterology and Hepatology, Erasmus MC-University Medical 
      Center, Rotterdam, the Netherlands.
FAU - van Spriel, Annemiek B
AU  - van Spriel AB
AD  - Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, 
      Radboud University Medical Center, Nijmegen, The Netherlands.
LA  - eng
PT  - Journal Article
DEP - 20170907
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (CD37 protein, human)
RN  - 0 (Kangai-1 Protein)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tetraspanin 25)
RN  - 0 (Tetraspanin 28)
RN  - 0 (Tetraspanins)
SB  - IM
MH  - Animals
MH  - Antigens, Neoplasm/genetics
MH  - Cells, Cultured
MH  - Dendritic Cells/*metabolism
MH  - Flow Cytometry
MH  - Humans
MH  - Kangai-1 Protein/genetics
MH  - Leukocytes, Mononuclear/metabolism
MH  - Mice
MH  - Protein Binding
MH  - RNA, Messenger/genetics
MH  - Tetraspanin 25/genetics
MH  - Tetraspanin 28/genetics
MH  - Tetraspanins/genetics/*metabolism
PMC - PMC5589240
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/09/08 06:00
MHDA- 2017/10/17 06:00
PMCR- 2017/09/07
CRDT- 2017/09/08 06:00
PHST- 2017/03/26 00:00 [received]
PHST- 2017/08/22 00:00 [accepted]
PHST- 2017/09/08 06:00 [entrez]
PHST- 2017/09/08 06:00 [pubmed]
PHST- 2017/10/17 06:00 [medline]
PHST- 2017/09/07 00:00 [pmc-release]
AID - PONE-D-17-11472 [pii]
AID - 10.1371/journal.pone.0184317 [doi]
PST - epublish
SO  - PLoS One. 2017 Sep 7;12(9):e0184317. doi: 10.1371/journal.pone.0184317. 
      eCollection 2017.