PMID- 28881496
OWN - NLM
STAT- MEDLINE
DCOM- 20171124
LR  - 20180320
IS  - 1742-4658 (Electronic)
IS  - 1742-464X (Linking)
VI  - 284
IP  - 21
DP  - 2017 Nov
TI  - Xk-related protein 8 regulates myoblast differentiation and survival.
PG  - 3575-3588
LID - 10.1111/febs.14261 [doi]
AB  - Xk-related protein 8 (Xkr8) is a scramblase and responsible for 
      phosphatidylserine (PS) exposure on the cell surface in a caspase-dependent 
      manner. Although PS exposure is found to be important for myotube formation 
      during myoblast differentiation, the role of Xkr8 during myogenesis has not been 
      elucidated. Here we show that Xkr8 contributes to myoblast differentiation. Xkr8 
      overexpression induced the formation of large myotubes during early 
      differentiation, but this phenotype was not related to caspase-dependent cleavage 
      of Xkr8. Furthermore, forced Xkr8 expression accelerated myoblast differentiation 
      and conferred cell-death resistance after the induction of differentiation. 
      Consistent with these results, Xkr8-knocked-down myoblasts exhibited impaired 
      differentiation and more apoptotic cells during differentiation, implying the 
      involvements of Xkr8 in the survival and proliferation of myoblasts. Taken 
      together, the study shows Xkr8 influences myogenesis by acting as a positive 
      regulator of terminal differentiation and myoblast survival.
CI  - (c) 2017 Federation of European Biochemical Societies.
FAU - Kim, Go-Woon
AU  - Kim GW
AD  - Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science 
      and Technology (KIST), Seoul, Korea.
FAU - Nam, Gi-Hoon
AU  - Nam GH
AD  - Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science 
      and Technology (KIST), Seoul, Korea.
AD  - KU-KIST Graduate School of Converging Science and Technology, Korea University, 
      Seoul, Korea.
FAU - Kim, In-San
AU  - Kim IS
AD  - Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science 
      and Technology (KIST), Seoul, Korea.
AD  - KU-KIST Graduate School of Converging Science and Technology, Korea University, 
      Seoul, Korea.
FAU - Park, Seung-Yoon
AU  - Park SY
AD  - Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science 
      and Technology (KIST), Seoul, Korea.
AD  - Department of Biochemistry, School of Medicine, Dongguk University, Gyeongju, 
      Korea.
LA  - eng
PT  - Editorial
PT  - Research Support, Non-U.S. Gov't
DEP - 20170928
PL  - England
TA  - FEBS J
JT  - The FEBS journal
JID - 101229646
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Xkr8 protein, human)
RN  - 0 (Xkr8 protein, mouse)
SB  - IM
CIN - FEBS J. 2017 Nov;284(21):3573-3574. PMID: 29120098
MH  - Animals
MH  - Apoptosis Regulatory Proteins/genetics/*metabolism
MH  - Cell Differentiation
MH  - Cell Survival
MH  - Humans
MH  - Membrane Proteins/genetics/*metabolism
MH  - Mice
MH  - Myoblasts/*cytology/*metabolism
MH  - RNA, Messenger/genetics/metabolism
OTO - NOTNLM
OT  - Xk-related protein 8
OT  - apoptosis
OT  - differentiation
OT  - myoblasts
OT  - scramblase
EDAT- 2017/09/08 06:00
MHDA- 2017/11/29 06:00
CRDT- 2017/09/08 06:00
PHST- 2017/04/03 00:00 [received]
PHST- 2017/07/26 00:00 [revised]
PHST- 2017/09/05 00:00 [accepted]
PHST- 2017/09/08 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2017/09/08 06:00 [entrez]
AID - 10.1111/febs.14261 [doi]
PST - ppublish
SO  - FEBS J. 2017 Nov;284(21):3575-3588. doi: 10.1111/febs.14261. Epub 2017 Sep 28.