PMID- 28881581
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20170925
LR  - 20181113
IS  - 1949-2553 (Electronic)
IS  - 1949-2553 (Linking)
VI  - 8
IP  - 31
DP  - 2017 Aug 1
TI  - A molecular pathology method for sequential fluorescence in situ hybridization 
      for multi-gene analysis at the single-cell level.
PG  - 50534-50541
LID - 10.18632/oncotarget.10245 [doi]
AB  - Multi-gene detection at the single-cell level is desirable to enable more precise 
      genotyping of heterogeneous hematology and oncology samples. This study aimed to 
      establish a single-cell multi-gene fluorescence in situ hybridization (FISH) 
      method for use in molecular pathology analyses. Five fluorochromes were used to 
      label different FISH gene probes, and 5 genes were detected using a five-color 
      FISH protocol. After the first hybridization, the previous FISH probe set was 
      stripped, and a second set of five-color FISH probes was used for 
      rehybridization. After each hybridization, the fluorescence signals were recorded 
      in 6 fluorescence filter channels that included DAPI, Spectrum Green(), Cy3() 
      v1, Texas Red, Cy5, and PF-415. A digital automatic relocation procedure was used 
      to ensure that exactly the same microscopic field was studied in each stripping 
      and hybridization cycle. By using this sequential stripping and rehybridization 
      strategy, up to 20 genes can be detected within a single nucleus. In conclusion, 
      a practical molecular pathology method was developed for analyzing multiple genes 
      at the single-cell level.
FAU - Hu, Linping
AU  - Hu L
AD  - State Key Laboratory of Experimental Hematology, Institute of Hematology, Blood 
      Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical 
      College, Tianjin China.
FAU - Yin, Xiuxiu
AU  - Yin X
AD  - State Key Laboratory of Experimental Hematology, Institute of Hematology, Blood 
      Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical 
      College, Tianjin China.
FAU - Sun, Jiangman
AU  - Sun J
AD  - State Key Laboratory of Experimental Hematology, Institute of Hematology, Blood 
      Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical 
      College, Tianjin China.
FAU - Zetterberg, Anders
AU  - Zetterberg A
AD  - The Department of Oncology-Pathology, Karolinska Cancer Institute, Karolinska 
      Institute, Stockholm, Sweden.
FAU - Miao, Weimin
AU  - Miao W
AD  - State Key Laboratory of Experimental Hematology, Institute of Hematology, Blood 
      Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical 
      College, Tianjin China.
AD  - Union Stem Cell and Gene Engineering Co. Ltd, Tianjin China.
FAU - Cheng, Tao
AU  - Cheng T
AD  - State Key Laboratory of Experimental Hematology, Institute of Hematology, Blood 
      Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical 
      College, Tianjin China.
LA  - eng
PT  - Journal Article
DEP - 20160623
PL  - United States
TA  - Oncotarget
JT  - Oncotarget
JID - 101532965
PMC - PMC5584163
OTO - NOTNLM
OT  - genotyping
OT  - molecular pathology
OT  - multi-gene detection
OT  - sequential FISH
OT  - single cell
COIS- CONFLICTS OF INTEREST All authors declare no conflicts of interest.
EDAT- 2016/06/23 00:00
MHDA- 2016/06/23 00:01
PMCR- 2017/08/01
CRDT- 2017/09/09 06:00
PHST- 2015/11/10 00:00 [received]
PHST- 2016/05/01 00:00 [accepted]
PHST- 2017/09/09 06:00 [entrez]
PHST- 2016/06/23 00:00 [pubmed]
PHST- 2016/06/23 00:01 [medline]
PHST- 2017/08/01 00:00 [pmc-release]
AID - 10245 [pii]
AID - 10.18632/oncotarget.10245 [doi]
PST - epublish
SO  - Oncotarget. 2016 Jun 23;8(31):50534-50541. doi: 10.18632/oncotarget.10245. 
      eCollection 2017 Aug 1.