PMID- 28882569
OWN - NLM
STAT- MEDLINE
DCOM- 20171018
LR  - 20171018
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 636
DP  - 2017 Dec 15
TI  - miR-130a regulates differential lipid accumulation between intramuscular and 
      subcutaneous adipose tissues of pigs via suppressing PPARG expression.
PG  - 23-29
LID - S0378-1119(17)30674-1 [pii]
LID - 10.1016/j.gene.2017.08.036 [doi]
AB  - To investigate the molecular mechanism underlying differential lipid deposition 
      between intramuscular (IM) and subcutaneous (SC) fat tissues of pigs, peroxisome 
      proliferator-activated receptor gamma (PPARG) expression levels were compared in 
      these two types of adipose tissues. The results showed that both mRNA and protein 
      levels of PPARG were significantly higher in SC fat than in IM fat. 
      Correspondingly, the expression levels of miR-128 and miR-130a, which potentially 
      targeting PPARG 3'-untranslated region (3'-UTR), in IM fat were 8.37 and 
      2.30-fold of those in SC fat respectively. Further dual luciferase activity assay 
      showed that only miR-130a directly targeting PPARG. Moreover, over-expression of 
      miR-130a in preadipocytes significantly inhibited its differentiation by 
      suppressing PPARG expression. In conclusion, tissue variance of miR-130a levels 
      results in the diverse of PPARG, and might be the reason for differential fat 
      deposition between IM and SC fat tissues in pigs. Our study would provide the 
      molecular foundation for IM fat deposition increase and therefore contribute to 
      pork quality improvement.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Wei, Wei
AU  - Wei W
AD  - College of Animal Science and Technology, Nanjing Agricultural University, 
      Nanjing, Jiangsu 210095, China.
FAU - Sun, Wenxing
AU  - Sun W
AD  - College of Public Health, Nantong University, Nantong, Jiangsu 226019, China.
FAU - Han, Haiyin
AU  - Han H
AD  - College of Animal Science and Technology, Nanjing Agricultural University, 
      Nanjing, Jiangsu 210095, China.
FAU - Chu, Weiwei
AU  - Chu W
AD  - College of Animal Science and Technology, Nanjing Agricultural University, 
      Nanjing, Jiangsu 210095, China.
FAU - Zhang, Lifan
AU  - Zhang L
AD  - College of Animal Science and Technology, Nanjing Agricultural University, 
      Nanjing, Jiangsu 210095, China.
FAU - Chen, Jie
AU  - Chen J
AD  - College of Animal Science and Technology, Nanjing Agricultural University, 
      Nanjing, Jiangsu 210095, China. Electronic address: jiechen@njau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20170904
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Lipids)
RN  - 0 (MicroRNAs)
RN  - 0 (PPAR gamma)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Adipocytes/cytology/metabolism
MH  - Adipose Tissue/*metabolism
MH  - Animals
MH  - Cell Differentiation
MH  - *Gene Expression Regulation
MH  - Lipids
MH  - Male
MH  - MicroRNAs/*metabolism
MH  - PPAR gamma/*genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Stem Cells/cytology/metabolism
MH  - Subcutaneous Fat/*metabolism
MH  - Sus scrofa/*genetics/metabolism
OTO - NOTNLM
OT  - Fat deposition
OT  - PPARG
OT  - Pork quality
OT  - miR-130a
EDAT- 2017/09/09 06:00
MHDA- 2017/10/19 06:00
CRDT- 2017/09/09 06:00
PHST- 2017/03/03 00:00 [received]
PHST- 2017/08/17 00:00 [revised]
PHST- 2017/08/24 00:00 [accepted]
PHST- 2017/09/09 06:00 [pubmed]
PHST- 2017/10/19 06:00 [medline]
PHST- 2017/09/09 06:00 [entrez]
AID - S0378-1119(17)30674-1 [pii]
AID - 10.1016/j.gene.2017.08.036 [doi]
PST - ppublish
SO  - Gene. 2017 Dec 15;636:23-29. doi: 10.1016/j.gene.2017.08.036. Epub 2017 Sep 4.