PMID- 28884389
OWN - NLM
STAT- MEDLINE
DCOM- 20190114
LR  - 20200217
IS  - 1614-7499 (Electronic)
IS  - 0944-1344 (Print)
IS  - 0944-1344 (Linking)
VI  - 25
IP  - 32
DP  - 2018 Nov
TI  - Microbial communities in pesticide-contaminated soils in Kyrgyzstan and 
      bioremediation possibilities.
PG  - 31848-31862
LID - 10.1007/s11356-017-0048-5 [doi]
AB  - In Kyrgyzstan, many former storehouses and dump sites for obsolete pesticides 
      exist. In 2009/2010, an inventory and assessment of these sites including risks 
      of environmental hazard has been conducted by FAO and the World Bank. Monitoring 
      revealed high concentration of pesticides listed as persistent organic pollutants 
      (POPs). The purpose of this research was to study the microbial structural 
      complexes of the pesticide-contaminated soils in these dumping zones, and to 
      search for and select microorganism's destructors with cytochrome P450 genes for 
      pesticide degradation. Culture-dependent and culture-independent approaches were 
      used to determine the taxonomic composition of these bacterial communities. The 
      universal primer set for the 16S ribosomal RNA (rRNA) gene and the specific 
      primer set P450R were used to amplify the cytochrome P450 hydroxylase gene. In 
      soils from Suzak A and B and soils from Balykchy dumping sites, the bacteria from 
      the Actinobacteria phylum (Micrococcus genus) were dominant. These bacteria made 
      up 32-47% of the indigenous local microflora; bacteria species from the 
      Pseudomonas genus (Gammaproteobacteria phylum) made up 23% in Suzak, 12% in 
      Balykchy soils. Bacillus species from the Firmicutes phylum were found only in 
      Suzak soils. The 16S rRNA analyses and the specific primer set P450R have 
      revealed bacteria with cytochrome genes which are directly involved in the 
      degradation process of organic carbon compounds. Experiments were carried out to 
      help select active degraders from the bacterial populations isolated and used to 
      degrade Aldrin in laboratory. Active bacterial strains from the Pseudomonas 
      fluorescens and Bacillus polymyxa population were selected which demonstrated 
      high rates of degradation activity on Aldrin.
FAU - Doolotkeldieva, Tinatin
AU  - Doolotkeldieva T
AD  - Plant Protection Department, Kyrgyz-Turkish Manas University, 56 Prospect Mira, 
      Bishkek, Kyrgyzstan. tdoolotkeldieva@gmail.com.
FAU - Konurbaeva, Maxabat
AU  - Konurbaeva M
AD  - Plant Protection Department, Kyrgyz-Turkish Manas University, 56 Prospect Mira, 
      Bishkek, Kyrgyzstan.
FAU - Bobusheva, Saykal
AU  - Bobusheva S
AD  - Plant Protection Department, Kyrgyz-Turkish Manas University, 56 Prospect Mira, 
      Bishkek, Kyrgyzstan.
LA  - eng
GR  - the project "Bioremediation of environmental contaminants by 
      microorganisms"/Science Foundation of the Ministry of Education and Science of 
      the Kyrgyz Republic/
PT  - Journal Article
DEP - 20170907
PL  - Germany
TA  - Environ Sci Pollut Res Int
JT  - Environmental science and pollution research international
JID - 9441769
RN  - 0 (Pesticides)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Soil Pollutants)
SB  - IM
MH  - Bacteria/classification/genetics/isolation & purification/*metabolism
MH  - Biodegradation, Environmental
MH  - Kyrgyzstan
MH  - *Microbiota
MH  - Pesticides/analysis/*metabolism
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Soil Microbiology
MH  - Soil Pollutants/analysis/*metabolism
PMC - PMC6208721
OTO - NOTNLM
OT  - Bacterial populations with P450 cytochrome genes, active bacteria for aerobic 
      bioremediation of pesticides
OT  - Contaminated soil
OT  - Microbial consortia
OT  - Obsolete pesticide dump
EDAT- 2017/09/09 06:00
MHDA- 2019/01/15 06:00
PMCR- 2017/09/07
CRDT- 2017/09/09 06:00
PHST- 2016/05/02 00:00 [received]
PHST- 2017/08/29 00:00 [accepted]
PHST- 2017/09/09 06:00 [pubmed]
PHST- 2019/01/15 06:00 [medline]
PHST- 2017/09/09 06:00 [entrez]
PHST- 2017/09/07 00:00 [pmc-release]
AID - 10.1007/s11356-017-0048-5 [pii]
AID - 48 [pii]
AID - 10.1007/s11356-017-0048-5 [doi]
PST - ppublish
SO  - Environ Sci Pollut Res Int. 2018 Nov;25(32):31848-31862. doi: 
      10.1007/s11356-017-0048-5. Epub 2017 Sep 7.