PMID- 28884834
OWN - NLM
STAT- MEDLINE
DCOM- 20190227
LR  - 20190227
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 119
IP  - 6
DP  - 2018 Jun
TI  - Transplantation of hypoxic preconditioned neural stem cells benefits functional 
      recovery via enhancing neurotrophic secretion after spinal cord injury in rats.
PG  - 4339-4351
LID - 10.1002/jcb.26397 [doi]
AB  - Spinal cord injury (SCI) is a debilitating, costly, and common pathological 
      condition that affects the function of central nervous system (CNS). To date, 
      there are few promising therapeutic strategies available for SCI. To look for a 
      suitable therapeutic strategy, we have developed a sublethal hypoxic 
      preconditioning procedure using Fluorescence-activated cell sorting (FACS) 
      analysis, LDH releasing, and cell viability assays in vitro. Meanwhile, we have 
      examined the benefits of neural stem cells (NSCs) transplantation prior to 
      hypoxic preconditioning on functional recovery and potential mechanism via MRI 
      screening, H&E, and Nissl staining, immunofluorescence staining and Elisa assays. 
      Our data showed that transplantation of hypoxic prconditioned NSCs could enhance 
      neuronal survival, especially 5-TH(+) and ChAT(+) neurons, in the injured spinal 
      cord to reinforce functional benefits. The hypoxia exposure upregulated HIF-1alpha, 
      neurotrophic and growth factors including neurotrophin-3 (NT-3), glial 
      cell-derived neurotrophic factor (GDNF), and brain-derived neurotrophic factor 
      (BDNF) in vitro and in vivo. Furthermore, functional recovery, including 
      locomotor and hypersensitivities to mechanical and thermal stimulation assessed 
      via behavioral and sensory tests, improved significantly in rats with engraftment 
      of NSCs after hypoxia exposure from day 14 post-SCI, compared with the control 
      and N-NSCs groups. In short, the approach employed in this study could result in 
      functional recovery via upregulating neurotrophic and growth factors, which 
      implies that hypoxic preconditioning strategy could serve as an effective and 
      feasible strategy for cell-based therapy in the treatment of SCI in rats.
CI  - (c) 2017 Wiley Periodicals, Inc.
FAU - Fan, Wei-Li
AU  - Fan WL
AD  - Department of Spinal Surgery, Daping Hospital, Research Institute of Surgery, The 
      Third Military Medical University, Chongqing, China.
FAU - Liu, Peng
AU  - Liu P
AD  - Department of Spinal Surgery, Daping Hospital, Research Institute of Surgery, The 
      Third Military Medical University, Chongqing, China.
FAU - Wang, Guan
AU  - Wang G
AD  - Department of Spinal Surgery, Daping Hospital, Research Institute of Surgery, The 
      Third Military Medical University, Chongqing, China.
FAU - Pu, Jun-Gang
AU  - Pu JG
AD  - Department of Spinal Surgery, Daping Hospital, Research Institute of Surgery, The 
      Third Military Medical University, Chongqing, China.
FAU - Xue, Xin
AU  - Xue X
AD  - Department of Spinal Surgery, Daping Hospital, Research Institute of Surgery, The 
      Third Military Medical University, Chongqing, China.
FAU - Zhao, Jian-Hua
AU  - Zhao JH
AUID- ORCID: 0000-0003-4393-4818
AD  - Department of Spinal Surgery, Daping Hospital, Research Institute of Surgery, The 
      Third Military Medical University, Chongqing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180228
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Nerve Growth Factors)
SB  - IM
MH  - Animals
MH  - Female
MH  - *Ischemic Preconditioning
MH  - *Locomotion
MH  - Nerve Growth Factors/*metabolism
MH  - Neural Stem Cells/*metabolism/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Recovery of Function
MH  - *Spinal Cord Injuries/metabolism/pathology/physiopathology/therapy
MH  - *Stem Cell Transplantation
OTO - NOTNLM
OT  - hypoxic preconditioning
OT  - neural stem cells
OT  - neurotrophic factors
OT  - spinal cord injury
EDAT- 2017/09/09 06:00
MHDA- 2019/02/28 06:00
CRDT- 2017/09/09 06:00
PHST- 2017/04/21 00:00 [received]
PHST- 2017/08/30 00:00 [accepted]
PHST- 2017/09/09 06:00 [pubmed]
PHST- 2019/02/28 06:00 [medline]
PHST- 2017/09/09 06:00 [entrez]
AID - 10.1002/jcb.26397 [doi]
PST - ppublish
SO  - J Cell Biochem. 2018 Jun;119(6):4339-4351. doi: 10.1002/jcb.26397. Epub 2018 Feb 
      28.