PMID- 28885660
OWN - NLM
STAT- MEDLINE
DCOM- 20180831
LR  - 20181202
IS  - 1724-6008 (Electronic)
IS  - 0393-6155 (Linking)
VI  - 33
IP  - 1
DP  - 2018 Jan
TI  - Cortactin and phosphorylated cortactin tyr(421) and tyr(466) expression in 
      supraglottic laryngeal carcinomas and lymph node metastases.
PG  - 79-86
LID - 10.5301/ijbm.5000297 [doi]
AB  - BACKGROUND: The most important adverse prognostic factor for laryngeal squamous 
      cell carcinoma (LSCC) is the presence of cervical lymph node metastases. The 
      supraglottic area of the larynx is richly supplied with lymphatics, and 25%-75% 
      of supraglottic carcinomas metastasize in neck lymph nodes. Cortactin is a 
      multidomain protein related to actin cytoskeleton regulation, podosome and 
      lamellipodia formation, integrin signaling, axon guidance and extracellular 
      matrix degradation. Cortactin is involved in metastasis formation because of its 
      role in cell mobility. The present study focused mainly on the role of cortactin 
      and phosphorylated cortactin (residues tyr(421) and tyr(466)) expression and 
      subcellular localization in primary supraglottic LSCCs and their cervical lymph 
      node metastases. METHODS: The immunohistochemical expression of cortactin, 
      p-Y466-cortactin and p-Y421-cortactin was assessed in 38 primary supraglottic 
      LSCCs and 10 lymph node metastases. The statistical approach included 
      bootstrapping analysis. RESULTS: Despite a significantly higher expression of 
      cortactin in carcinoma cells than in adjacent normal laryngeal mucosa, no 
      associations emerged between prognosis and the expression of cortactin or its 
      isoforms in supraglottic LSCC. Statistical analysis found cortactin expression 
      higher in less-differentiated LSCCs (p = 0.03). A significant direct correlation 
      was found between cortactin and p-Y466-cortactin levels (p = 0.031), and between 
      p-Y466-cortactin and p-Y421-cortactin levels (p = 0.001). CONCLUSIONS: Cortactin 
      expression in carcinoma cells and its known involvement in the EGFR pathway 
      suggest a role for this protein as a target for LSCC therapy. Further prospective 
      studies are needed to investigate the potential of cortactin, p-Y466-cortactin 
      and p-Y421-cortactin expression as markers of response to treatment (particularly 
      EGFR-directed agents) in LSCC.
FAU - Marioni, Gino
AU  - Marioni G
AD  - 1 Department of Neurosciences, Otolaryngology Section, University of Padua, Padua 
      - Italy.
FAU - Lionello, Marco
AU  - Lionello M
AD  - 2 Otolaryngology Unit, Vittorio Veneto Hospital, Vittorio Veneto - Italy.
FAU - Marchese-Ragona, Rosario
AU  - Marchese-Ragona R
AD  - 1 Department of Neurosciences, Otolaryngology Section, University of Padua, Padua 
      - Italy.
FAU - Fasanaro, Elena
AU  - Fasanaro E
AD  - 3 Department of Radiotherapy, Veneto Institute of Oncology IOV-IRCCS, Padua - 
      Italy.
FAU - Valentini, Elisa
AU  - Valentini E
AD  - 4 Department of Medicine DIMED, University of Padua, Padua - Italy.
FAU - Zanoletti, Elisabetta
AU  - Zanoletti E
AD  - 1 Department of Neurosciences, Otolaryngology Section, University of Padua, Padua 
      - Italy.
FAU - Stritoni, Paola
AU  - Stritoni P
AD  - 1 Department of Neurosciences, Otolaryngology Section, University of Padua, Padua 
      - Italy.
FAU - Ramacciotti, Giulia
AU  - Ramacciotti G
AD  - 1 Department of Neurosciences, Otolaryngology Section, University of Padua, Padua 
      - Italy.
AD  - 5 Department of Otolaryngology-Head and Neck Surgery, VU University Medical 
      Center, Amsterdam - The Netherlands.
FAU - Guzzardo, Vincenza
AU  - Guzzardo V
AD  - 4 Department of Medicine DIMED, University of Padua, Padua - Italy.
FAU - Giacomelli, Luciano
AU  - Giacomelli L
AD  - 4 Department of Medicine DIMED, University of Padua, Padua - Italy.
FAU - Ralli, Giovanni
AU  - Ralli G
AD  - 6 Department of Sensory Organs, Policlinico Umberto I, Sapienza University of 
      Rome, Rome - Italy.
FAU - Ottaviano, Giancarlo
AU  - Ottaviano G
AD  - 1 Department of Neurosciences, Otolaryngology Section, University of Padua, Padua 
      - Italy.
FAU - Blandamura, Stella
AU  - Blandamura S
AD  - 4 Department of Medicine DIMED, University of Padua, Padua - Italy.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Int J Biol Markers
JT  - The International journal of biological markers
JID - 8712411
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Cortactin)
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/genetics
MH  - Carcinoma/*genetics/pathology
MH  - Cortactin/*genetics
MH  - ErbB Receptors/*genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Lymph Nodes/pathology
MH  - Lymphatic Metastasis
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Phosphorylation
MH  - Prognosis
OTO - NOTNLM
OT  - Cortactin
OT  - Laryngeal
OT  - Lymph node metastases
OT  - Phosphorylated
OT  - Squamous cell carcinoma
OT  - Supraglottic
EDAT- 2017/09/09 06:00
MHDA- 2018/09/01 06:00
CRDT- 2017/09/09 06:00
PHST- 2017/09/09 06:00 [pubmed]
PHST- 2018/09/01 06:00 [medline]
PHST- 2017/09/09 06:00 [entrez]
AID - D9AC40DA-438E-4FD1-ACCA-A2FF40630FB5 [pii]
AID - 10.5301/ijbm.5000297 [doi]
PST - ppublish
SO  - Int J Biol Markers. 2018 Jan;33(1):79-86. doi: 10.5301/ijbm.5000297.