PMID- 28886271
OWN - NLM
STAT- MEDLINE
DCOM- 20180705
LR  - 20240327
IS  - 1555-8576 (Electronic)
IS  - 1538-4047 (Print)
IS  - 1538-4047 (Linking)
VI  - 18
IP  - 10
DP  - 2017 Oct 3
TI  - MicroRNA signature in the chemoprevention of functionally-enriched stem and 
      progenitor pools (FESPP) by Active Hexose Correlated Compound (AHCC).
PG  - 765-774
LID - 10.1080/15384047.2017.1373211 [doi]
AB  - PURPOSE: Many breast cancer patients use natural compounds in their battle 
      against breast cancer. Active Hexose Correlated Compound (AHCC(R)) is a cultured 
      mushroom mycelium extract shown to favorably modulate the immune system and 
      alleviate cancer burden. Cancer Stem cells (CSCs) are a subset of highly 
      tumorigenic cancer cells that are thought to be responsible for recurrence. CSCs 
      can be epigenetically regulated by microRNAs (miRNAs). We hypothesized that AHCC 
      may influence CSCs by modulating tumor-suppressor or oncogenic miRNAs. METHODS: 
      Functionally-enriched stem and progenitor pools (FESPP) were isolated in the form 
      of mammospheres from MDA-MB-231, MCF-7, and 4T1 cells, exposed to AHCC in both 
      regular and primary culture from Balb/c mice, and analyzed by visual counting and 
      flow cytometry. Cell motility was also observed in MDA-MB-231 cells. Profiling 
      and RT-qPCR were performed to determine AHCC influence on miRNAs in MDA-MB-231 
      mammospheres. Additionally, Balb/c mice were orally gavaged with AHCC, and tumor 
      growth parameters and miR-335 expression were analyzed. MDA-MB-231 cells were 
      transfected with miR-335 and analyzed by western blot. RESULTS: We demonstrated 
      that AHCC reduced mammosphere growth in three cell lines and in primary culture, 
      prevented cell migration, and upregulated miR-335 expression in MDA-MB-231 cells 
      and mouse tumor samples. Among the differentially regulated miRNAs in CSCs, we 
      focused on tumor suppressor miR-335, known to target extracellular matrix protein 
      Tenascin C (TNC). TNC is involved in CSC immune evasion pathways. In MDA-MB-231, 
      inhibition of miR-335 increased TNC protein expression. CONCLUSIONS: These 
      results support that AHCC limits FESPP growth, partly by targeting miRNA 
      pathways.
FAU - Graham, Emilie A
AU  - Graham EA
AD  - a Interdisciplinary Health Sciences , University of Ottawa , Ottawa , Canada.
FAU - Mallet, Jean-Francois
AU  - Mallet JF
AD  - b Cellular and Molecular Medicine, Faculty of Medicine , University of Ottawa , 
      Ottawa, Ontario , Canada.
FAU - Jambi, Majed
AU  - Jambi M
AD  - b Cellular and Molecular Medicine, Faculty of Medicine , University of Ottawa , 
      Ottawa, Ontario , Canada.
FAU - Nishioka, Hiroshi
AU  - Nishioka H
AD  - c R&D Division Amino Up Chemical Co, Ltd , Sapporo , Japan.
FAU - Homma, Kohei
AU  - Homma K
AD  - c R&D Division Amino Up Chemical Co, Ltd , Sapporo , Japan.
FAU - Matar, Chantal
AU  - Matar C
AD  - a Interdisciplinary Health Sciences , University of Ottawa , Ottawa , Canada.
AD  - b Cellular and Molecular Medicine, Faculty of Medicine , University of Ottawa , 
      Ottawa, Ontario , Canada.
LA  - eng
PT  - Journal Article
DEP - 20170908
PL  - United States
TA  - Cancer Biol Ther
JT  - Cancer biology & therapy
JID - 101137842
RN  - 0 (Immunologic Factors)
RN  - 0 (MIRN335 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn335 microRNA, mouse)
RN  - 0 (Polysaccharides)
RN  - 0 (Tenascin)
RN  - S1W5KTD68Y (Active Hexose Correlated Compound)
SB  - IM
MH  - Animals
MH  - Breast Neoplasms/genetics/immunology/*prevention & control
MH  - Carcinogenesis/drug effects/genetics/immunology
MH  - Cell Line, Tumor
MH  - Cell Movement/drug effects
MH  - Cell Proliferation/drug effects
MH  - Epigenesis, Genetic/drug effects
MH  - Female
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic/*drug effects
MH  - Humans
MH  - Immunologic Factors/*pharmacology/therapeutic use
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/genetics/immunology/metabolism
MH  - Neoplastic Stem Cells/*drug effects/immunology/metabolism
MH  - Polysaccharides/*pharmacology/therapeutic use
MH  - Primary Cell Culture
MH  - Tenascin/genetics/immunology/metabolism
MH  - Up-Regulation
MH  - Xenograft Model Antitumor Assays
PMC - PMC5678688
OTO - NOTNLM
OT  - AHCC
OT  - Tenascin C
OT  - breast cancer
OT  - cancer stem cell
OT  - microRNA
EDAT- 2017/09/09 06:00
MHDA- 2018/07/06 06:00
PMCR- 2018/09/08
CRDT- 2017/09/09 06:00
PHST- 2017/09/09 06:00 [pubmed]
PHST- 2018/07/06 06:00 [medline]
PHST- 2017/09/09 06:00 [entrez]
PHST- 2018/09/08 00:00 [pmc-release]
AID - 1373211 [pii]
AID - 10.1080/15384047.2017.1373211 [doi]
PST - ppublish
SO  - Cancer Biol Ther. 2017 Oct 3;18(10):765-774. doi: 10.1080/15384047.2017.1373211. 
      Epub 2017 Sep 8.