PMID- 28886936 OWN - NLM STAT- MEDLINE DCOM- 20171228 LR - 20181202 IS - 1873-3778 (Electronic) IS - 0021-9673 (Linking) VI - 1519 DP - 2017 Oct 13 TI - Quantification of piperazine in chicken and pig tissues by gas chromatography-electron ionization tandem mass spectrometry employing pre-column derivatization with acetic anhydride. PG - 9-18 LID - S0021-9673(17)31273-6 [pii] LID - 10.1016/j.chroma.2017.08.079 [doi] AB - This paper describes a novel method that combines acetic anhydride derivatization with gas chromatography-electron ionization tandem mass spectrometry (GC-EI/MS/MS) for the sensitive and selective determination of piperazine in chicken and pig tissues. Samples were extracted using an accelerated solvent extraction (ASE) apparatus, purified by solid-phase extraction (SPE) and derivatized with acetic anhydride. This optimized method was validated according to the requirements defined by the European Union and the Food and Drug Administration. At the limit of quantification (LOQ) spiked levels of 50.0, 100.0, 500.0, 1000.0 and 2000.0mug/kg, the average recoveries of piperazine in chicken and pig tissues were 77.46-96.26%, with relative standard deviations (RSDs) of 1.55-6.64%. The intra-day RSDs were 1.39-5.92%, and the inter-day RSDs were 2.24-8.39%. The limits of detection (LODs) and the LOQs were 1.4-1.6mug/kg and 4.8-5.2mug/kg, respectively. The decision limits (CC(alpha)) were 102.02-105.17mug/kg, and the detection capabilities (CC(beta)) were 104.03-109.09mug/kg. Finally, the new approach was verified for the quantitative determination of piperazine in 30 commercial chicken and pig tissues from local supermarkets. CI - Copyright (c) 2017. Published by Elsevier B.V. FAU - Wang, Bo AU - Wang B AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, China. FAU - Pang, Maoda AU - Pang M AD - Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. FAU - Xie, Xing AU - Xie X AD - Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. FAU - Xie, Kaizhou AU - Xie K AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, China. Electronic address: yzxkz168@163.com. FAU - Zhang, Yangyang AU - Zhang Y AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, China. FAU - Cui, Lulu AU - Cui L AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, China. FAU - Zhao, Xia AU - Zhao X AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, China. FAU - Wang, Yajuan AU - Wang Y AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, China. FAU - Shi, Huiqiang AU - Shi H AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, China. FAU - Guo, Yawen AU - Guo Y AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, China. FAU - Wang, Ran AU - Wang R AD - Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. FAU - Zhang, Genxi AU - Zhang G AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, China. FAU - Dai, Guojun AU - Dai G AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, China. FAU - Wang, Jinyu AU - Wang J AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, China. LA - eng PT - Journal Article DEP - 20170901 PL - Netherlands TA - J Chromatogr A JT - Journal of chromatography. A JID - 9318488 RN - 0 (Acetic Anhydrides) RN - 0 (Piperazines) RN - 2E48G1QI9Q (acetic anhydride) SB - IM MH - Acetic Anhydrides/chemistry MH - Animals MH - Chickens MH - Food Analysis/*methods MH - *Gas Chromatography-Mass Spectrometry MH - Limit of Detection MH - Meat/*analysis MH - Piperazines/*analysis MH - Solid Phase Extraction MH - Sus scrofa MH - Swine OTO - NOTNLM OT - Chicken tissues OT - GC-EI/MS/MS OT - Pig tissues OT - Piperazine OT - Pre-column derivatization OT - Residue EDAT- 2017/09/10 06:00 MHDA- 2017/12/29 06:00 CRDT- 2017/09/10 06:00 PHST- 2017/07/12 00:00 [received] PHST- 2017/08/23 00:00 [revised] PHST- 2017/08/24 00:00 [accepted] PHST- 2017/09/10 06:00 [pubmed] PHST- 2017/12/29 06:00 [medline] PHST- 2017/09/10 06:00 [entrez] AID - S0021-9673(17)31273-6 [pii] AID - 10.1016/j.chroma.2017.08.079 [doi] PST - ppublish SO - J Chromatogr A. 2017 Oct 13;1519:9-18. doi: 10.1016/j.chroma.2017.08.079. Epub 2017 Sep 1.