PMID- 28887190
OWN - NLM
STAT- MEDLINE
DCOM- 20180522
LR  - 20231213
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 249
DP  - 2017 Nov
TI  - Rapid detection of fifteen known soybean viruses by dot-immunobinding assay.
PG  - 126-129
LID - S0166-0934(17)30384-1 [pii]
LID - 10.1016/j.jviromet.2017.09.003 [doi]
AB  - A dot-immunobinding assay (DIBA) was optimized and used successfully for the 
      rapid detection of 15 known viruses [Alfalfa mosaic virus (AMV), Bean pod mottle 
      virus (BPMV), Bean yellow mosaic virus (BYMV), Cowpea mild mottle virus (CPMMV), 
      Cowpea severe mosaic virus (CPSMV), Cucumber mosaic virus (CMV), Peanut mottle 
      virus (PeMoV), Peanut stunt virus (PSV), Southern bean mosaic virus (SBMV), 
      Soybean dwarf virus (SbDV), Soybean mosaic virus (SMV), Soybean vein necrosis 
      virus (SVNV), Tobacco ringspot virus (TRSV), Tomato ringspot virus (ToRSV), and 
      Tobacco streak virus (TSV)] infecting soybean plants in Oklahoma. More than 1000 
      leaf samples were collected in approximately 100 commercial soybean fields in 24 
      counties of Oklahoma, during the 2012-2013 growing seasons. All samples were 
      tested by DIBA using polyclonal antibodies of the above 15 plant viruses. 
      Thirteen viruses were detected, and 8 of them were reported for the first time in 
      soybean crops of Oklahoma. The highest average incidence was recorded for PeMoV 
      (13.5%) followed by SVNV (6.9%), TSV (6.4%), BYMV, (4.5%), and TRSV (3.9%), while 
      the remaining seven viruses were detected in less than 2% of the samples tested. 
      The DIBA was quick, and economical to screen more than 1000 samples against 15 
      known plant viruses in a very short time.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Ali, Akhtar
AU  - Ali A
AD  - Department of Biological Science, The University of Tulsa, Tulsa, Oklahoma, 
      74104, USA. Electronic address: Akhtar-ali@utulsa.edu.
LA  - eng
PT  - Journal Article
DEP - 20170906
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Antibodies, Viral)
RN  - Soybean mosaic virus
SB  - IM
MH  - Antibodies, Viral/immunology
MH  - Carlavirus/immunology/isolation & purification
MH  - Comovirus/immunology/isolation & purification
MH  - Cucumovirus/immunology/isolation & purification
MH  - Ilarvirus/immunology/isolation & purification
MH  - Immunoassay/economics/*methods
MH  - Nepovirus/immunology/isolation & purification
MH  - Oklahoma
MH  - Plant Diseases/virology
MH  - Plant Leaves/virology
MH  - Plant Viruses/immunology/*isolation & purification
MH  - Potyvirus/immunology/isolation & purification
MH  - Glycine max/*virology
OTO - NOTNLM
OT  - DIBA
OT  - Serological detection
OT  - Soybean viruses
EDAT- 2017/09/10 06:00
MHDA- 2018/05/23 06:00
CRDT- 2017/09/10 06:00
PHST- 2017/06/11 00:00 [received]
PHST- 2017/08/16 00:00 [revised]
PHST- 2017/09/05 00:00 [accepted]
PHST- 2017/09/10 06:00 [pubmed]
PHST- 2018/05/23 06:00 [medline]
PHST- 2017/09/10 06:00 [entrez]
AID - S0166-0934(17)30384-1 [pii]
AID - 10.1016/j.jviromet.2017.09.003 [doi]
PST - ppublish
SO  - J Virol Methods. 2017 Nov;249:126-129. doi: 10.1016/j.jviromet.2017.09.003. Epub 
      2017 Sep 6.