PMID- 28888894
OWN - NLM
STAT- MEDLINE
DCOM- 20180706
LR  - 20231112
IS  - 2213-2317 (Electronic)
IS  - 2213-2317 (Linking)
VI  - 14
DP  - 2018 Apr
TI  - Exercise intervention attenuates hyperhomocysteinemia-induced aortic endothelial 
      oxidative injury by regulating SIRT1 through mitigating NADPH oxidase/LOX-1 
      signaling.
PG  - 116-125
LID - S2213-2317(17)30561-X [pii]
LID - 10.1016/j.redox.2017.08.016 [doi]
AB  - Coronary artery disease (CAD) is a critical cardiovascular disease and a cause of 
      high morbidity and mortality in this world. Hyperhomocysteinemia (HHcy) has been 
      suggested as a risk factor for CAD. In addition, SIRT1 (sirtuin 1) has been 
      reported to play a protective role in a variety of diseases, especially in the 
      cardiovascular system. The main purpose of this study was to investigate the 
      effects of exercise training on apoptosis and inflammation in HHcy animals. We 
      also tested whether exercise protected against Hhcy-induced dysfunction of 
      endothelium through modulation of SIRT1. C57BL mice (8 in each group) were fed 
      with or without 1% L-methionine (w/w) in water for 4 months to induce HHcy. We 
      found that Hhcy repressed SIRT1 and AMPK expression and increased NADPH oxidase 
      activity. Plasma MDA, endothelium LOX-1 and p-p38 were up-regulated by Hhcy 
      induction. NF-kappaB and it downstream molecules were activated under Hhcy situation, 
      thereby promoting pro-inflammatory responses. Moreover, we also reported that 
      Hhcy caused endothelium apoptosis involving Akt inhibition and 
      mitochondria-dependent apoptotic pathways. Exercise training significantly 
      protected against endothelium from Hhcy caused oxidative injuries. In addition, 
      EX527 (SIRT1 inhibitor) reduced the therapeutic effects by exercise. Our results 
      had indicated that exercise training prevent the development of atherosclerosis 
      through SIRT1 activation and oxidative stress inhibition under Hhcy situation.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Chan, Shih-Hung
AU  - Chan SH
AD  - Department of Internal Medicine, National Cheng Kung University Hospital, College 
      of Medicine, National Cheng Kung University, Tainan, Taiwan.
FAU - Hung, Ching-Hsia
AU  - Hung CH
AD  - Department of Physical Therapy, College of Medicine, National Cheng Kung 
      University, Tainan, Taiwan; Institute of Allied Health Sciences, College of 
      Medicine, National Cheng Kung University, Tainan, Taiwan.
FAU - Shih, Jhih-Yuan
AU  - Shih JY
AD  - Department of Internal Medicine, Chi-Mei Hospital, Tainan, Taiwan.
FAU - Chu, Pei-Ming
AU  - Chu PM
AD  - Department of Anatomy, School of Medicine, China Medical University, Taichung, 
      Taiwan.
FAU - Cheng, Yung-Hsin
AU  - Cheng YH
AD  - Department of Education and Research, Taipei City Hospital, Taipei, Taiwan.
FAU - Lin, Huei-Chen
AU  - Lin HC
AD  - Institute of Allied Health Sciences, College of Medicine, National Cheng Kung 
      University, Tainan, Taiwan; Department of Physical Therapy, Shu-Zen Junior 
      College of Medicine and Management, Taiwan.
FAU - Hsieh, Pei-Ling
AU  - Hsieh PL
AD  - Institute of Oral Sciences, Chung Shan Medical University, Taichung, Taiwan.
FAU - Tsai, Kun-Ling
AU  - Tsai KL
AD  - Department of Physical Therapy, College of Medicine, National Cheng Kung 
      University, Tainan, Taiwan. Electronic address: kunlingtsai@mail.ncku.edu.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170824
PL  - Netherlands
TA  - Redox Biol
JT  - Redox biology
JID - 101605639
RN  - 0 (6-chloro-2,3,4,9-tetrahydro-1H-carbazole-1-carboxamide)
RN  - 0 (Carbazoles)
RN  - 0 (NF-kappa B)
RN  - 0 (Neuropeptides)
RN  - 0 (Olr1 protein, mouse)
RN  - 0 (Rac1 protein, mouse)
RN  - 0 (Scavenger Receptors, Class E)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - AE28F7PNPL (Methionine)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.6.3.- (NADPH Oxidase 1)
RN  - EC 3.5.1.- (Sirtuin 1)
RN  - EC 3.6.5.2 (rac1 GTP-Binding Protein)
SB  - IM
MH  - Animals
MH  - Atherosclerosis/etiology/prevention & control
MH  - Carbazoles/pharmacology/therapeutic use
MH  - Endothelium, Vascular/drug effects/metabolism
MH  - Hyperhomocysteinemia/chemically induced/complications/drug therapy
MH  - Male
MH  - Malondialdehyde/blood
MH  - Methionine/toxicity
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NADPH Oxidase 1/antagonists & inhibitors/*metabolism
MH  - NF-kappa B/metabolism
MH  - Neuropeptides/metabolism
MH  - Oxidative Stress/drug effects
MH  - Physical Conditioning, Animal
MH  - Scavenger Receptors, Class E/*metabolism
MH  - Signal Transduction/drug effects
MH  - Sirtuin 1/antagonists & inhibitors/*metabolism
MH  - Superoxide Dismutase/blood
MH  - Up-Regulation/drug effects
MH  - rac1 GTP-Binding Protein/metabolism
PMC - PMC5596261
OTO - NOTNLM
OT  - Coronary artery disease
OT  - Hyperhomocysteinemia
OT  - Oxidative stress
OT  - Sirtuin 1
EDAT- 2017/09/11 06:00
MHDA- 2018/07/07 06:00
PMCR- 2017/08/24
CRDT- 2017/09/11 06:00
PHST- 2017/07/28 00:00 [received]
PHST- 2017/08/18 00:00 [revised]
PHST- 2017/08/23 00:00 [accepted]
PHST- 2017/09/11 06:00 [pubmed]
PHST- 2018/07/07 06:00 [medline]
PHST- 2017/09/11 06:00 [entrez]
PHST- 2017/08/24 00:00 [pmc-release]
AID - S2213-2317(17)30561-X [pii]
AID - 10.1016/j.redox.2017.08.016 [doi]
PST - ppublish
SO  - Redox Biol. 2018 Apr;14:116-125. doi: 10.1016/j.redox.2017.08.016. Epub 2017 Aug 
      24.