PMID- 28890655
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1177-3901 (Print)
IS  - 1177-3901 (Electronic)
IS  - 1177-3901 (Linking)
VI  - 12
DP  - 2017
TI  - Comparison of 3 Derivatization Methods for the Analysis of Amphetamine-Related 
      Drugs in Oral Fluid by Gas Chromatography-Mass Spectrometry.
PG  - 1177390117727533
LID - 10.1177/1177390117727533 [doi]
LID - 1177390117727533
AB  - The heptafluorobutyric anhydride (HFBA), pentafluoropropionic anhydride (PFPA), 
      and trifluoroacetic anhydride (TFAA) are compared as derivatizing reagents to use 
      as the optimal method for the analysis of 10 amphetamines and cathinones in oral 
      fluid. The target compounds were amphetamine (AMP), methamphetamine (MA), 
      4-methylamphetamine, 3,4-methylenedioxyamphetamine (MDA), 
      3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxy-N-ethylamphetamine 
      (MDEA), cathinone (CAT), methcathinone, mephedrone, and ephedrine. 
      Amphetamine-D(5), MA-D(5), MDA-D(5), MDMA-D(5), and MDEA-D(5) use as internal 
      standards (IS). The analytes and IS were extracted from 0.5 mL of oral fluid by 
      ethyl acetate in the presence of NaOH (0.1 N) as the base and then the dried 
      extracts were derivatized with HFBA, PFPA, or TFAA at 70 degrees C for 30 minutes. The 
      limits of quantification based on signal-to-noise ratios >/=10 were ranged between 
      2.5 and 10 ng/mL. The calibration graphs were linear in the range of 5 or 10 to 
      1000 ng/mL for all analytes. Based on sensitivity, the PFPA is proved to be the 
      best for derivatization of the target compounds prior to gas chromatography-mass 
      spectrometry analysis.
FAU - Mohamed, Khaled M
AU  - Mohamed KM
AD  - The Department of Forensic Chemistry, The College of Forensic Sciences, Naif Arab 
      University for Security Sciences, Riyadh, Saudi Arabia.
FAU - Bakdash, Abdulsallam
AU  - Bakdash A
AD  - The Department of Forensic Chemistry, The College of Forensic Sciences, Naif Arab 
      University for Security Sciences, Riyadh, Saudi Arabia.
LA  - eng
PT  - Journal Article
DEP - 20170824
PL  - United States
TA  - Anal Chem Insights
JT  - Analytical chemistry insights
JID - 101463741
PMC - PMC5574487
OTO - NOTNLM
OT  - Amphetamine
OT  - analysis
OT  - drugs
OT  - gas chromatography
OT  - oral fluids
COIS- Declaration of conflicting interests:The author(s) declared no potential 
      conflicts of interest with respect to the research, authorship, and/or 
      publication of this article.
EDAT- 2017/09/12 06:00
MHDA- 2017/09/12 06:01
PMCR- 2017/08/24
CRDT- 2017/09/12 06:00
PHST- 2017/04/09 00:00 [received]
PHST- 2017/07/26 00:00 [accepted]
PHST- 2017/09/12 06:00 [entrez]
PHST- 2017/09/12 06:00 [pubmed]
PHST- 2017/09/12 06:01 [medline]
PHST- 2017/08/24 00:00 [pmc-release]
AID - 10.1177_1177390117727533 [pii]
AID - 10.1177/1177390117727533 [doi]
PST - epublish
SO  - Anal Chem Insights. 2017 Aug 24;12:1177390117727533. doi: 
      10.1177/1177390117727533. eCollection 2017.