PMID- 28893265
OWN - NLM
STAT- MEDLINE
DCOM- 20180524
LR  - 20231213
IS  - 1476-4598 (Electronic)
IS  - 1476-4598 (Linking)
VI  - 16
IP  - 1
DP  - 2017 Sep 11
TI  - Circular RNA_LARP4 inhibits cell proliferation and invasion of gastric cancer by 
      sponging miR-424-5p and regulating LATS1 expression.
PG  - 151
LID - 10.1186/s12943-017-0719-3 [doi]
LID - 151
AB  - BACKGROUND: Non-coding RNAs (ncRNAs) have been shown to regulate gene expression 
      involved in tumor progression of multiple malignancies. Our previous studies 
      indicated that large tumor suppressor kinase 1 (LATS1), a core part of Hippo 
      signaling pathway, functions as a tumor suppressor in gastric cancer (GC). But, 
      the underlying molecular mechanisms by which ncRNAs modulate LATS1 expression in 
      GC remain undetermined. METHODS: The correlation of LATS1 and has-miR-424-5p 
      (miR-424) expression with clinicopathological characteristics and prognosis of GC 
      patients was analyzed by TCGA RNA-sequencing data. A novel circular RNA_LARP4 
      (circLARP4) was identified to sponge miR-424 by circRNA expression profile and 
      bioinformatic analysis. The binding site between miR-424 and LATS1 or circLARP4 
      was verified using dual luciferase assay and RNA immunoprecipitation (RIP) assay. 
      The expression and localization of circLARP4 in GC tissues were investigated by 
      fluorescence in situ hybridization (FISH). MTT, colony formation, Transwell and 
      EdU assays were performed to assess the effects of miR-424 or circLARP4 on cell 
      proliferation and invasion. RESULTS: Increased miR-424 expression or decreased 
      LATS1 expression was associated with pathological stage and unfavorable prognosis 
      of GC patients. Ectopic expression of miR-424 promoted proliferation and invasion 
      of GC cells by targeting LATS1 gene. Furthermore, circLARP4 was mainly localized 
      in the cytoplasm and inhibited biological behaviors of GC cells by sponging 
      miR-424. The expression of circLARP4 was downregulated in GC tissues and 
      represented an independent prognostic factor for overall survival of GC patients. 
      CONCLUSION: circLARP4 may act as a novel tumor suppressive factor and a potential 
      biomarker in GC.
FAU - Zhang, Jing
AU  - Zhang J
AD  - Department of Gastroenterology, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, No. 600 Yishan Road, Shanghai, 200233, China.
FAU - Liu, Hui
AU  - Liu H
AD  - Department of Gastroenterology, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, No. 600 Yishan Road, Shanghai, 200233, China.
FAU - Hou, Lidan
AU  - Hou L
AD  - Department of Gastroenterology, Shanghai Ninth People's Hospital, Shanghai Jiao 
      Tong University School of Medicine, Shanghai, China.
FAU - Wang, Ge
AU  - Wang G
AD  - Department of Gastroenterology, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, No. 600 Yishan Road, Shanghai, 200233, China.
FAU - Zhang, Rui
AU  - Zhang R
AD  - Department of Gastroenterology, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, No. 600 Yishan Road, Shanghai, 200233, China.
FAU - Huang, Yanxia
AU  - Huang Y
AD  - Department of Gastroenterology, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, No. 600 Yishan Road, Shanghai, 200233, China.
FAU - Chen, Xiaoyu
AU  - Chen X
AD  - Department of Gastroenterology, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, No. 600 Yishan Road, Shanghai, 200233, China.
FAU - Zhu, Jinshui
AU  - Zhu J
AD  - Department of Gastroenterology, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, No. 600 Yishan Road, Shanghai, 200233, China. 
      zhujs1803@163.com.
LA  - eng
PT  - Journal Article
DEP - 20170911
PL  - England
TA  - Mol Cancer
JT  - Molecular cancer
JID - 101147698
RN  - 0 (Autoantigens)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (MIRN424 microrna, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - 0 (Ribonucleoproteins)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.1.- (LATS1 protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Autoantigens/*genetics
MH  - Biomarkers, Tumor
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Cell Survival/genetics
MH  - Ectopic Gene Expression
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - Neoplasm Grading
MH  - Neoplasm Invasiveness
MH  - Neoplasm Staging
MH  - Prognosis
MH  - Protein Serine-Threonine Kinases/*genetics
MH  - RNA/*genetics
MH  - RNA Interference
MH  - RNA, Circular
MH  - ROC Curve
MH  - Reproducibility of Results
MH  - Ribonucleoproteins/*genetics
MH  - Stomach Neoplasms/*genetics/mortality/*pathology
MH  - SS-B Antigen
PMC - PMC5594516
OTO - NOTNLM
OT  - Circular RNA_LARP4
OT  - Gastric cancer
OT  - Invasion
OT  - LATS1
OT  - miR-424-5p
COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: The present study was approved by the 
      Hospital's Protection of Human Subjects Committee. COMPETING INTERESTS: The 
      authors declare that they have no competing interests. PUBLISHER'S NOTE: Springer 
      Nature remains neutral with regard to jurisdictional claims in published maps and 
      institutional affiliations.
EDAT- 2017/09/13 06:00
MHDA- 2018/05/25 06:00
PMCR- 2017/09/11
CRDT- 2017/09/13 06:00
PHST- 2017/05/25 00:00 [received]
PHST- 2017/08/31 00:00 [accepted]
PHST- 2017/09/13 06:00 [entrez]
PHST- 2017/09/13 06:00 [pubmed]
PHST- 2018/05/25 06:00 [medline]
PHST- 2017/09/11 00:00 [pmc-release]
AID - 10.1186/s12943-017-0719-3 [pii]
AID - 719 [pii]
AID - 10.1186/s12943-017-0719-3 [doi]
PST - epublish
SO  - Mol Cancer. 2017 Sep 11;16(1):151. doi: 10.1186/s12943-017-0719-3.