PMID- 28898406
OWN - NLM
STAT- MEDLINE
DCOM- 20171222
LR  - 20220321
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Print)
IS  - 0009-9104 (Linking)
VI  - 191
IP  - 1
DP  - 2018 Jan
TI  - DC-SIGN reacts with TLR-4 and regulates inflammatory cytokine expression via 
      NF-kappaB activation in renal tubular epithelial cells during acute renal injury.
PG  - 107-115
LID - 10.1111/cei.13048 [doi]
AB  - In the pathological process of acute kidney injury (AKI), innate immune receptors 
      are essential in inflammatory response modulation; however, the precise molecular 
      mechanisms are still unclear. Our study sought to demonstrate the inflammatory 
      response mechanisms in renal tubular epithelial cells via Toll-like receptor-4 
      (TLR-4) and dendritic cell-specific intercellular adhesion molecule 3-grabbing 
      non-integrin 1 (DC-SIGN) signalling. We found that DC-SIGN exhibited strong 
      expression in renal tubular epithelial cells of human acute renal injury tissues. 
      DC-SIGN protein expression was increased significantly when renal tubular 
      epithelial cells were exposed to lipopolysaccharide (LPS) for a short period. 
      Furthermore, DC-SIGN was involved in the activation of p65 by TLR-4, which 
      excluded p38 and c-Jun N-terminal kinases (JNK). Interleukin (IL)-6 and tumour 
      necrosis factor (TNF)-alpha expression was decreased after DC-SIGN knock-down, and 
      LPS induced endogenous interactions and plasma membrane co-expression between 
      TLR-4 and DC-SIGN. These results show that DC-SIGN and TLR-4 interactions 
      regulate inflammatory responses in renal tubular epithelial cells and participate 
      in AKI pathogenesis.
CI  - (c) 2017 British Society for Immunology.
FAU - Feng, D
AU  - Feng D
AD  - Department of Pediatrics, Ruijin Hospital, Shanghai, China.
FAU - Wang, Y
AU  - Wang Y
AD  - Institute of Cardiovascular Disease, Ruijin Hospital, Shanghai, China.
FAU - Liu, Y
AU  - Liu Y
AD  - Institute of Cardiovascular Disease, Ruijin Hospital, Shanghai, China.
AD  - Department of Cardiology, Shanghai Ninth People's Hospital, Shanghai, China.
FAU - Wu, L
AU  - Wu L
AD  - Institute of Cardiovascular Disease, Ruijin Hospital, Shanghai, China.
FAU - Li, X
AU  - Li X
AD  - Department of Nephrology, Ruijin Hospital, Shanghai Jiaotong University School of 
      Medicine, Shanghai, China.
FAU - Chen, Y
AU  - Chen Y
AD  - Department of Pediatrics, Ruijin Hospital, Shanghai, China.
FAU - Chen, Y
AU  - Chen Y
AD  - Institute of Cardiovascular Disease, Ruijin Hospital, Shanghai, China.
FAU - Chen, Y
AU  - Chen Y
AD  - Institute of Cardiovascular Disease, Ruijin Hospital, Shanghai, China.
FAU - Xu, C
AU  - Xu C
AD  - Department of Pediatrics, Ruijin Hospital, Shanghai, China.
FAU - Yang, K
AU  - Yang K
AD  - Institute of Cardiovascular Disease, Ruijin Hospital, Shanghai, China.
FAU - Zhou, T
AU  - Zhou T
AUID- ORCID: 0000-0002-1178-2613
AD  - Department of Pediatrics, Ruijin Hospital, Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171005
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Biomarkers)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Cytokines)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Acute Kidney Injury/genetics/*metabolism
MH  - Biomarkers
MH  - Cell Adhesion Molecules/genetics/*metabolism
MH  - Cell Line
MH  - Cytokines/genetics/*metabolism
MH  - Epithelial Cells/metabolism
MH  - Gene Expression
MH  - Gene Knockdown Techniques
MH  - Gene Silencing
MH  - Humans
MH  - Immunohistochemistry
MH  - Inflammation Mediators/*metabolism
MH  - Kidney Tubules/*metabolism
MH  - Lectins, C-Type/genetics/*metabolism
MH  - Myeloid Differentiation Factor 88/metabolism
MH  - NF-kappa B/*metabolism
MH  - RNA, Small Interfering/genetics
MH  - Receptors, Cell Surface/genetics/*metabolism
MH  - Toll-Like Receptor 4/*metabolism
PMC - PMC5721233
OTO - NOTNLM
OT  - DC-SIGN
OT  - NF-kappaB
OT  - TLR-4
OT  - acute kidney injury
OT  - renal tubular epithelial cells
EDAT- 2017/09/13 06:00
MHDA- 2017/12/23 06:00
PMCR- 2019/01/01
CRDT- 2017/09/13 06:00
PHST- 2017/09/06 00:00 [accepted]
PHST- 2017/09/13 06:00 [pubmed]
PHST- 2017/12/23 06:00 [medline]
PHST- 2017/09/13 06:00 [entrez]
PHST- 2019/01/01 00:00 [pmc-release]
AID - CEI13048 [pii]
AID - 10.1111/cei.13048 [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2018 Jan;191(1):107-115. doi: 10.1111/cei.13048. Epub 2017 Oct 
      5.