PMID- 28899944
OWN - NLM
STAT- MEDLINE
DCOM- 20171018
LR  - 20181113
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 474
IP  - 20
DP  - 2017 Oct 10
TI  - Novel endogenous retrovirus-derived transcript expressed in the bovine placenta 
      is regulated by WNT signaling.
PG  - 3499-3512
LID - 10.1042/BCJ20170531 [doi]
AB  - Endogenous retroviruses (ERVs) are involved in placentation; perhaps, the most 
      well-known ERVs are the syncytins, actively transcribed env genes involved in 
      cell-cell fusion and possible morphological variations. However, ERVs other than 
      syncytins that play an important role in placental development have not been well 
      characterized. To identify ERV genes expressed during the onset of placentation 
      in the bovine species, we characterized the expression profiles of bovine 
      conceptus transcripts during the peri-attachment period using RNA-seq analysis, 
      and confirming some candidates through real-time PCR. Using in silico and PCR 
      analyses, we identified a novel ERV proviral sequence derived from a gag region, 
      designated bovine endogenous retroviruses (BERV)-K3, containing Gag_p10 and 
      Gag_p24, zinc finger domain. Initial expression of this ERV in bovine conceptuses 
      was on day 20 (day 0 = day of estrus), soon after conceptus attachment to the 
      endometrial epithelium, and its high placental expression was maintained up to 
      the middle of pregnancy. The BERV-K3 transcript was also found in the uterine 
      luminal and glandular epithelia, liver, kidney, intestine, and skin. BERV-K3 is 
      located on chromosome 7 and integrated within LOC100848658, from which noncoding 
      RNA could be transcribed. Furthermore, the expression of endogenous BERV-K3 in 
      bovine trophoblast cell lines was induced by a WNT agonist, a signaling system 
      common to genes expressed in placentas. These data support the argument that 
      during the evolutionary process, mammals incorporated not only similar ERV 
      sequences, but also ERVs unique to individual species. BERV-K3 is in the latter 
      case, likely providing functions unique to ruminant gestation.
CI  - (c) 2017 The Author(s).
FAU - Sakurai, Toshihiro
AU  - Sakurai T
AD  - Department of Occupational and Environment Health, Tokyo University of Science, 
      Chiba 278-0022, Japan.
FAU - Nakagawa, So
AU  - Nakagawa S
AD  - Department of Molecular Life Science, Tokai University School of Medicine, 
      Kanagawa 259-1193, Japan.
FAU - Bai, Hanako
AU  - Bai H
AD  - Department of Animal Science, Hokkaido University, Hokkaido 060-8589, Japan.
FAU - Bai, Rulan
AU  - Bai R
AD  - Animal Resource Science Center, Graduate School of Agricultural and Life 
      Sciences, The University of Tokyo, Ibaraki 319-0206, Japan.
FAU - Kusama, Kazuya
AU  - Kusama K
AD  - Animal Resource Science Center, Graduate School of Agricultural and Life 
      Sciences, The University of Tokyo, Ibaraki 319-0206, Japan 
      akaz@mail.ecc.u-tokyo.ac.jp.
FAU - Ideta, Atsushi
AU  - Ideta A
AD  - Zen-noh Embryo Transfer Center, Kamishihoro, Hokkaido 080-1407, Japan.
FAU - Aoyagi, Yoshito
AU  - Aoyagi Y
AD  - Zen-noh Embryo Transfer Center, Kamishihoro, Hokkaido 080-1407, Japan.
FAU - Kaneko, Kazuyuki
AU  - Kaneko K
AD  - Laboratory of Theriogenology, Department of Veterinary Medicine, Azabu 
      University, Kanagawa 252-5201, Japan.
FAU - Iga, Kosuke
AU  - Iga K
AD  - Tohoku Agricultural Research Center, National Agriculture and Food Research 
      Organization, Iwate 020-0198, Japan.
FAU - Yasuda, Jiro
AU  - Yasuda J
AD  - Department of Emerging Infectious Diseases, Institute for Tropical Medicine, 
      Nagasaki University, Nagasaki 852-8523, Japan.
FAU - Miyazawa, Takayuki
AU  - Miyazawa T
AD  - Laboratory of Signal Transduction, Department of Cell Biology, Institute for 
      Viral Research, Kyoto University, Kyoto 606-8507, Japan.
FAU - Imakawa, Kazuhiko
AU  - Imakawa K
AD  - Animal Resource Science Center, Graduate School of Agricultural and Life 
      Sciences, The University of Tokyo, Ibaraki 319-0206, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171010
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Endogenous Retroviruses/*genetics
MH  - Female
MH  - *Gene Expression Regulation, Developmental
MH  - Placenta/*physiology
MH  - Pregnancy
MH  - Transcription, Genetic/*physiology
MH  - Wnt Signaling Pathway/*physiology
PMC - PMC5633919
OTO - NOTNLM
OT  - bovine
OT  - endogenous retrovirus
OT  - placentation
COIS- The Authors declare that there are no competing interests associated with the 
      manuscript.
EDAT- 2017/09/14 06:00
MHDA- 2017/10/19 06:00
PMCR- 2017/10/10
CRDT- 2017/09/14 06:00
PHST- 2017/07/03 00:00 [received]
PHST- 2017/08/22 00:00 [revised]
PHST- 2017/09/07 00:00 [accepted]
PHST- 2017/09/14 06:00 [pubmed]
PHST- 2017/10/19 06:00 [medline]
PHST- 2017/09/14 06:00 [entrez]
PHST- 2017/10/10 00:00 [pmc-release]
AID - BCJ20170531 [pii]
AID - BCJ-474-3499 [pii]
AID - 10.1042/BCJ20170531 [doi]
PST - epublish
SO  - Biochem J. 2017 Oct 10;474(20):3499-3512. doi: 10.1042/BCJ20170531.