PMID- 28901448
OWN - NLM
STAT- MEDLINE
DCOM- 20180604
LR  - 20180604
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 16
IP  - 5
DP  - 2017 Nov
TI  - Exposure of keratinocytes to non‑thermal dielectric barrier discharge plasma 
      increases the level of 8‑oxoguanine via inhibition of its repair enzyme.
PG  - 6870-6875
LID - 10.3892/mmr.2017.7454 [doi]
AB  - Oxidative stress enhances cellular DNA oxidation and may cause mutations in DNA 
      bases, including 8‑oxoguanine (8‑oxoG). Our recent study reported that exposure 
      of cells to non‑thermal dielectric barrier discharge (DBD) plasma generates 
      reactive oxygen species and damages DNA. The present study investigated the 
      effect of non‑thermal DBD plasma exposure on the formation of 8‑oxoG in HaCaT 
      human keratinocytes. Cells exposed to DBD plasma exhibited increased level of 
      8‑oxoG. In addition, mRNA and protein expression levels of 8‑oxoguanine 
      glycosylase 1 (OGG1), an 8‑oxoG repair enzyme, were reduced in plasma‑exposed 
      cells. Furthermore, the expression level of nuclear factor erythroid 2‑related 
      factor 2 (Nrf2), a transcription factor that regulates OGG1 gene expression, was 
      reduced following exposure to DBD plasma. Pretreatment of cells with an 
      antioxidant, N‑acetyl cysteine (NAC), prior to plasma exposure suppressed the 
      formation of 8‑oxoG and restored the expression levels of OGG1 and Nrf2. In 
      addition, phosphorylation of protein kinase B (Akt), which regulates the 
      activation of Nrf2, was reduced following plasma exposure. However, 
      phosphorylation was restored by pretreatment with NAC. These findings suggested 
      that non‑thermal DBD plasma exposure generates 8‑oxoG via inhibition of the 
      Akt‑Nrf2‑OGG1 signaling pathway in HaCaT cells.
FAU - Kim, Ki Cheon
AU  - Kim KC
AD  - Department of Biochemistry, School of Medicine and Institute for Nuclear Science 
      and Technology, Jeju National University, Jeju 63243, Republic of Korea.
FAU - Ruwan Kumara, Madduma Hewage Susara
AU  - Ruwan Kumara MHS
AD  - Department of Biochemistry, School of Medicine and Institute for Nuclear Science 
      and Technology, Jeju National University, Jeju 63243, Republic of Korea.
FAU - Kang, Kyoung Ah
AU  - Kang KA
AD  - Department of Biochemistry, School of Medicine and Institute for Nuclear Science 
      and Technology, Jeju National University, Jeju 63243, Republic of Korea.
FAU - Piao, Mei Jing
AU  - Piao MJ
AD  - Department of Biochemistry, School of Medicine and Institute for Nuclear Science 
      and Technology, Jeju National University, Jeju 63243, Republic of Korea.
FAU - Oh, Min Chang
AU  - Oh MC
AD  - Department of Biochemistry, School of Medicine and Institute for Nuclear Science 
      and Technology, Jeju National University, Jeju 63243, Republic of Korea.
FAU - Ryu, Yea Seong
AU  - Ryu YS
AD  - Department of Biochemistry, School of Medicine and Institute for Nuclear Science 
      and Technology, Jeju National University, Jeju 63243, Republic of Korea.
FAU - Jo, Jin Oh
AU  - Jo JO
AD  - Department of Chemical and Biological Engineering, Jeju National University, Jeju 
      63243, Republic of Korea.
FAU - Mok, Young Sun
AU  - Mok YS
AD  - Department of Chemical and Biological Engineering, Jeju National University, Jeju 
      63243, Republic of Korea.
FAU - Shin, Jennifer H
AU  - Shin JH
AD  - Department of Mechanical Engineering, Korea Advanced Institute of Science and 
      Technology, Daejeon 34141, Republic of Korea.
FAU - Park, Yeunsoo
AU  - Park Y
AD  - Plasma Technology Research Center, National Fusion Research Institute, Gunsan 
      54004, Republic of Korea.
FAU - Kim, Seong Bong
AU  - Kim SB
AD  - Plasma Technology Research Center, National Fusion Research Institute, Gunsan 
      54004, Republic of Korea.
FAU - Yoo, Suk Jae
AU  - Yoo SJ
AD  - Plasma Technology Research Center, National Fusion Research Institute, Gunsan 
      54004, Republic of Korea.
FAU - Hyun, Jin Won
AU  - Hyun JW
AD  - Department of Biochemistry, School of Medicine and Institute for Nuclear Science 
      and Technology, Jeju National University, Jeju 63243, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20170911
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NFE2L2 protein, human)
RN  - 0 (Plasma Gases)
RN  - 0 (Reactive Oxygen Species)
RN  - 5614-64-2 (8-hydroxyguanine)
RN  - 5Z93L87A1R (Guanine)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 3.2.2.- (DNA Glycosylases)
RN  - EC 3.2.2.- (oxoguanine glycosylase 1, human)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/pharmacology
MH  - Cell Line
MH  - DNA/isolation & purification/metabolism
MH  - DNA Damage/*drug effects
MH  - DNA Glycosylases/genetics/*metabolism
MH  - Down-Regulation/*drug effects
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Guanine/*analogs & derivatives/analysis/metabolism
MH  - Humans
MH  - Keratinocytes/cytology/metabolism
MH  - NF-E2-Related Factor 2/genetics/metabolism
MH  - Oxidative Stress/drug effects
MH  - Phosphorylation/drug effects
MH  - Plasma Gases/*toxicity
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Up-Regulation/*drug effects
EDAT- 2017/09/14 06:00
MHDA- 2018/06/05 06:00
CRDT- 2017/09/14 06:00
PHST- 2016/01/22 00:00 [received]
PHST- 2017/01/27 00:00 [accepted]
PHST- 2017/09/14 06:00 [pubmed]
PHST- 2018/06/05 06:00 [medline]
PHST- 2017/09/14 06:00 [entrez]
AID - 10.3892/mmr.2017.7454 [doi]
PST - ppublish
SO  - Mol Med Rep. 2017 Nov;16(5):6870-6875. doi: 10.3892/mmr.2017.7454. Epub 2017 Sep 
      11.