PMID- 28910960
OWN - NLM
STAT- MEDLINE
DCOM- 20190426
LR  - 20190426
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 97
IP  - 34
DP  - 2017 Sep 12
TI  - [Effects of human mesenchymal stem cells on airway inflammation in allergic 
      asthma mice and the underlying mechanism].
PG  - 2697-2702
LID - 10.3760/cma.j.issn.0376-2491.2017.34.012 [doi]
AB  - Objectives: To investigate the effects of human umbilical cord mesenchymal stem 
      cells (hUC-MSCs) on airway inflammation in an ovalbumin (OVA) induced asthma 
      mouse model and the underlying mechanism. Methods: Twenty-four BALB/c mice were 
      randomly divided into four equal groups: normal control group, OVA-induced 
      asthmatic model group, hUC-MSCs treated group (50 mul of hUC-MSCs was transplanted 
      into the trachea of asthmatic mice ) and hUC-MSCs control group (50 mul of 
      hUC-MSCs was transplanted into the trachea of control mice). Human umbilical cord 
      mesenchymal stem cells from umbilical cord of healthy new born babies were used 
      as the source of hUC-MSCs for this study. The asthmatic conditions of the airways 
      and the lungs were assessed by examining: (1) histopathological changes of the 
      airways and the lungs; (2) expression of cytokines IL-6 and TGF-beta mRNA by 
      real-time PCR; (3) total leukocytes and mast cell count in bronchoalveolar lavage 
      fluid (BALF) and number of IL-17-expressing CD4(+) cells (Th17 cells) in the lung 
      tissue using flow cytometry. Results: Typical histopathological changes of asthma 
      were confirmed in the asthmatic model group. These changes included intensive 
      inflammatory cell infiltration around the airways and patchy airway occlusion by 
      hyperviscous mucus. The number of total leukocytes and mast cells in BALF were 
      significantly increased in the asthmatic mice when compared with the control 
      group (P<0.05). Mice in the asthmatic model group had significantly higher 
      percentage of Th17 cells in lung tissues when compared with the control group 
      (2.90% vs 0.76%, P<0.05). In contrast, in the asthmatic mice treated with 
      hUC-MSCs, the inflammatory cell infiltration was significantly reduced compared 
      with asthmatic mice, as observed by significantly lower leukocytes and mast cells 
      in BALF (P<0.05) and significant reduction in the percentage of Th17 cells in the 
      lung of OVA-challenged mice following hUC-MSCs treatment (percentage of Th17 
      cells: 0.24% vs 2.90%, P<0.05). The expression of mRNA for IL-6 and TGF-beta was 
      significantly suppressed in the hUC-MSCs treatment group (0.23 vs 2.30 and 0.56 
      vs 6.60, both P<0.01). No asthmatic pathological changes in both normal and 
      hUC-MSCs control groups were observed. Conclusions: hUC-MSCs significantly 
      inhibit the airway inflammation in OVA-induced asthmatic mice. This inhibition is 
      associated with the suppression of Th17 cells and the down-regulation of 
      inflammatory factors such as IL-6 and TGF-beta in the lungs.
FAU - Ren, N N
AU  - Ren NN
AD  - Guangdong Online Hospital, Guangdong 2nd Provincial People's Hospital, Guangzhou 
      510317, China.
FAU - Chen, H J
AU  - Chen HJ
FAU - Chen, Q L
AU  - Chen QL
FAU - Eileen, J
AU  - Eileen J
FAU - An, Y G
AU  - An YG
FAU - Lin, Yiguang
AU  - Lin Y
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - *Asthma
MH  - Bronchoalveolar Lavage Fluid
MH  - Disease Models, Animal
MH  - Humans
MH  - Hypersensitivity
MH  - Inflammation
MH  - *Mesenchymal Stem Cells
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Ovalbumin
OTO - NOTNLM
OT  - Asthma
OT  - Bronchitis
OT  - Mesenchymal stem cell transplantation
OT  - Mice
OT  - T-lymphocytes, helper-inducer
EDAT- 2017/09/16 06:00
MHDA- 2019/04/27 06:00
CRDT- 2017/09/15 06:00
PHST- 2017/09/15 06:00 [entrez]
PHST- 2017/09/16 06:00 [pubmed]
PHST- 2019/04/27 06:00 [medline]
AID - 10.3760/cma.j.issn.0376-2491.2017.34.012 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2017 Sep 12;97(34):2697-2702. doi: 
      10.3760/cma.j.issn.0376-2491.2017.34.012.