PMID- 28911115
OWN - NLM
STAT- MEDLINE
DCOM- 20171012
LR  - 20181113
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 45
IP  - 15
DP  - 2017 Sep 6
TI  - The IRES5'UTR of the dicistrovirus cricket paralysis virus is a type III IRES 
      containing an essential pseudoknot structure.
PG  - 8993-9004
LID - 10.1093/nar/gkx622 [doi]
AB  - Cricket paralysis virus (CrPV) is a dicistrovirus. Its positive-sense 
      single-stranded RNA genome contains two internal ribosomal entry sites (IRESs). 
      The 5' untranslated region (5'UTR) IRES5'UTR mediates translation of 
      non-structural proteins encoded by ORF1 whereas the well-known intergenic region 
      (IGR) IRESIGR is required for translation of structural proteins from open 
      reading frame 2 in the late phase of infection. Concerted action of both IRES is 
      essential for host translation shut-off and viral translation. IRESIGR has been 
      extensively studied, in contrast the IRES5'UTR remains largely unexplored. Here, 
      we define the minimal IRES element required for efficient translation initiation 
      in drosophila S2 cell-free extracts. We show that IRES5'UTR promotes direct 
      recruitment of the ribosome on the cognate viral AUG start codon without any 
      scanning step, using a Hepatitis-C virus-related translation initiation 
      mechanism. Mass spectrometry analysis revealed that IRES5'UTR recruits eukaryotic 
      initiation factor 3, confirming that it belongs to type III class of IRES 
      elements. Using Selective 2'-hydroxyl acylation analyzed by primer extension and 
      DMS probing, we established a secondary structure model of 5'UTR and of the 
      minimal IRES5'UTR. The IRES5'UTR contains a pseudoknot structure that is 
      essential for proper folding and ribosome recruitment. Overall, our results pave 
      the way for studies addressing the synergy and interplay between the two IRES 
      from CrPV.
CI  - (c) The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic 
      Acids Research.
FAU - Gross, Lauriane
AU  - Gross L
AD  - Universite de Strasbourg, CNRS, Architecture et Reactivite de l'ARN, UPR 9002, 
      F-67000 Strasbourg, France.
FAU - Vicens, Quentin
AU  - Vicens Q
AD  - Universite de Strasbourg, CNRS, Architecture et Reactivite de l'ARN, UPR 9002, 
      F-67000 Strasbourg, France.
FAU - Einhorn, Evelyne
AU  - Einhorn E
AD  - Universite de Strasbourg, CNRS, Reponse Immunitaire et Developpement chez les 
      Insectes, UPR 9022, F-67000 Strasbourg, France.
FAU - Noireterre, Audrey
AU  - Noireterre A
AD  - Universite de Strasbourg, CNRS, Architecture et Reactivite de l'ARN, UPR 9002, 
      F-67000 Strasbourg, France.
FAU - Schaeffer, Laure
AU  - Schaeffer L
AD  - Universite de Strasbourg, CNRS, Architecture et Reactivite de l'ARN, UPR 9002, 
      F-67000 Strasbourg, France.
FAU - Kuhn, Lauriane
AU  - Kuhn L
AD  - Universite de Strasbourg, CNRS, Plateforme Proteomique Strasbourg-Esplanade, 
      F-67000 Strasbourg, France.
FAU - Imler, Jean-Luc
AU  - Imler JL
AD  - Universite de Strasbourg, CNRS, Reponse Immunitaire et Developpement chez les 
      Insectes, UPR 9022, F-67000 Strasbourg, France.
FAU - Eriani, Gilbert
AU  - Eriani G
AD  - Universite de Strasbourg, CNRS, Architecture et Reactivite de l'ARN, UPR 9002, 
      F-67000 Strasbourg, France.
FAU - Meignin, Carine
AU  - Meignin C
AD  - Universite de Strasbourg, CNRS, Reponse Immunitaire et Developpement chez les 
      Insectes, UPR 9022, F-67000 Strasbourg, France.
FAU - Martin, Franck
AU  - Martin F
AD  - Universite de Strasbourg, CNRS, Architecture et Reactivite de l'ARN, UPR 9002, 
      F-67000 Strasbourg, France.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Internal Ribosome Entry Sites)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - *5' Untranslated Regions
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Cell-Free System/metabolism
MH  - Dicistroviridae/*genetics/growth & development/metabolism
MH  - Drosophila melanogaster/virology
MH  - Gryllidae/virology
MH  - Host-Pathogen Interactions
MH  - *Internal Ribosome Entry Sites
MH  - Nucleic Acid Conformation
MH  - Open Reading Frames
MH  - *Protein Biosynthesis
MH  - RNA, Viral/*chemistry/genetics/metabolism
MH  - Ribosomes/genetics/metabolism
MH  - Viral Proteins/*chemistry/genetics/metabolism
PMC - PMC5587806
EDAT- 2017/09/16 06:00
MHDA- 2017/10/13 06:00
PMCR- 2017/07/18
CRDT- 2017/09/16 06:00
PHST- 2016/11/03 00:00 [received]
PHST- 2017/07/07 00:00 [accepted]
PHST- 2017/09/16 06:00 [entrez]
PHST- 2017/09/16 06:00 [pubmed]
PHST- 2017/10/13 06:00 [medline]
PHST- 2017/07/18 00:00 [pmc-release]
AID - 3978035 [pii]
AID - gkx622 [pii]
AID - 10.1093/nar/gkx622 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 2017 Sep 6;45(15):8993-9004. doi: 10.1093/nar/gkx622.