PMID- 28912695
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1662-5145 (Print)
IS  - 1662-5145 (Electronic)
IS  - 1662-5145 (Linking)
VI  - 11
DP  - 2017
TI  - Region- and Cell-Specific Expression of Transmembrane Collagens in Mouse Brain.
PG  - 20
LID - 10.3389/fnint.2017.00020 [doi]
LID - 20
AB  - Unconventional collagens are nonfribrillar proteins that not only contribute to 
      the structure of extracellular matrices but exhibit unique bio-activities. 
      Although roles for unconventional collagens have been well-established in the 
      development and function of non-neural tissues, only recently have studies 
      identified roles for these proteins in brain development, and more specifically, 
      in the formation and refinement of synaptic connections between neurons. Still, 
      our understanding of the full cohort of unconventional collagens that are 
      generated in the mammalian brain remains unclear. Here, we sought to address this 
      gap by assessing the expression of transmembrane collagens (i.e., collagens XIII, 
      XVII, XXIII and XXV) in mouse brain. Using quantitative PCR and in situ 
      hybridization (ISH), we demonstrate both region- and cell-specific expression of 
      these unique collagens in the developing brain. For the two most highly expressed 
      transmembrane collagens (i.e., collagen XXIII and XXV), we demonstrate that they 
      are expressed by select subsets of neurons in different parts of the brain. For 
      example, collagen XXIII is selectively expressed by excitatory neurons in the 
      mitral/tufted cell layer of the accessory olfactory bulb (AOB) and by cells in 
      the inner nuclear layer (INL) of the retina. On the other hand, collagen XXV, 
      which is more broadly expressed, is generated by subsets of excitatory neurons in 
      the dorsal thalamus and midbrain and by inhibitory neurons in the retina, ventral 
      thalamus and telencephalon. Not only is col25a1 expression present in retina, it 
      appears specifically enriched in retino-recipient nuclei within the brain 
      (including the suprachiasmatic nucleus (SCN), lateral geniculate complex, olivary 
      pretectal nucleus (OPN) and superior colliculus). Taken together, the distinct 
      region- and cell-specific expression patterns of transmembrane collagens suggest 
      that this family of unconventional collagens may play unique, yet-to-be 
      identified roles in brain development and function.
FAU - Monavarfeshani, Aboozar
AU  - Monavarfeshani A
AD  - Developmental and Translational Neurobiology Center, Virginia Tech Carilion 
      Research InstituteRoanoke, VA, United States.
AD  - Department of Biological Sciences, Virginia TechBlacksburg, VA, United States.
FAU - Knill, Courtney N
AU  - Knill CN
AD  - Virginia Tech Carilion School of Medicine, Virginia TechRoanoke, VA, United 
      States.
FAU - Sabbagh, Ubadah
AU  - Sabbagh U
AD  - Developmental and Translational Neurobiology Center, Virginia Tech Carilion 
      Research InstituteRoanoke, VA, United States.
AD  - Translational Biology, Medicine, and Health Graduate Program, Virginia 
      TechBlacksburg, VA, United States.
FAU - Su, Jianmin
AU  - Su J
AD  - Developmental and Translational Neurobiology Center, Virginia Tech Carilion 
      Research InstituteRoanoke, VA, United States.
FAU - Fox, Michael A
AU  - Fox MA
AD  - Developmental and Translational Neurobiology Center, Virginia Tech Carilion 
      Research InstituteRoanoke, VA, United States.
AD  - Department of Biological Sciences, Virginia TechBlacksburg, VA, United States.
AD  - Department of Pediatrics, Virginia Tech Carilion School of MedicineRoanoke, VA, 
      United States.
LA  - eng
GR  - R01 EY021222/EY/NEI NIH HHS/United States
PT  - Journal Article
DEP - 20170830
PL  - Switzerland
TA  - Front Integr Neurosci
JT  - Frontiers in integrative neuroscience
JID - 101477950
PMC - PMC5583603
OTO - NOTNLM
OT  - accessory olfactory bulb
OT  - collagen
OT  - extracellular matrix
OT  - hippocampus
OT  - interneuron
OT  - lateral geniculate nucleus
OT  - retina
OT  - superior colliculus
EDAT- 2017/09/16 06:00
MHDA- 2017/09/16 06:01
PMCR- 2017/01/01
CRDT- 2017/09/16 06:00
PHST- 2017/05/31 00:00 [received]
PHST- 2017/08/16 00:00 [accepted]
PHST- 2017/09/16 06:00 [entrez]
PHST- 2017/09/16 06:00 [pubmed]
PHST- 2017/09/16 06:01 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - 10.3389/fnint.2017.00020 [doi]
PST - epublish
SO  - Front Integr Neurosci. 2017 Aug 30;11:20. doi: 10.3389/fnint.2017.00020. 
      eCollection 2017.